DES-Testbed, Freie Universität Research Bacteria,Pigeon,Plant,Polyclonal Application of DHPLC screening TGFBR-3 gene in Chinese women with idiopathic premature

Application of DHPLC screening TGFBR-3 gene in Chinese women with idiopathic premature


des-testbed

Software of DHPLC screening TGFBR-Three gene in Chinese language girls with idiopathic untimely ovarian failure

OBJECTIVE

To guage scientific worth of denaturing excessive efficiency liquid chromatography (DHPLC) utilized in detecting remodeling development issue beta receptor 3 (TGFBR-3) exons 11 and 12 polymorphism in girls with idiopathic untimely ovarian failure (POF).

METHODS

From Feb. 2009 to Dec. 2011, 110 sufferers with idiopathic POF present process therapy at Shenzhen Maternal & Youngster Well being Institute affiliated to Southern Medical College had been enrolled as POF group on this examine. At the moment, 110 girls beneath 40 years outdated with regular hormonal stage and menstrual cycles as management group. The exons 11 and 12 of TGFBR-3 gene polymorphism had been screened through the use of DHPLC, and outcomes of DNA sequencing was as golden commonplace.

Some associated indexes had been calculated, equivalent to sensitivity, specificity, false damaging worth, false optimistic worth, Youden index, optimistic predictive worth, and damaging predictive worth. On the similar time, 20% of the examined specimens had been chosen randomly and detected by DHPLC once more. The worth of Kappa index had been calculated by evaluating the outcomes between the primary and second DHPLC evaluation.

RESULTS

The exon 11 of TGFBR-Three weren’t recognized gene polymorphism and two nucleotide polymorphisms had been recognized in exon 12. For 2022 T/C polymorphism, the frequencies of CC with 0.9% (1/110), TC with 22.7% (25/110), TT with 76.4% (84/110), C with 12.3% (27/220) and T with 87.7% (193/220) in POF group had been considerably completely different from CC with 0, TC with 9.1% (10/110) and TT with 90.9% (100/110), C with 4.5% (10/220) and T with 95.5% (210/220) in management group (all P<0.05).

Allelic and genotypic frequencies of 2161-75 C/T weren’t differed considerably between the 2 teams (all P>0.05). As DNA sequencing as golden commonplace, DHPLC confirmed that the sensitivity was 100%, specificity was 97.9%, Youden index was 97.9%, optimistic predictive worth was 96.3%, damaging predictive worth was 100%, and Kappa index was 0.888 (P<0.05).

CONCLUSIONS

DHPLC evaluation is greater validity, reliability and practicability methodology in detecting TGFBR-3 polymorphism in idiopathic untimely ovarian failure.

des-testbed

des-testbed

Gls ELISA Kit| Rat Glutaminase kidney isoform, mitochondrial EL

EF018741 96 Tests
EUR 689

ELISA kit for Human Glutaminase kidney isoform, mitochondrial

EK3615 96 tests
EUR 670
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Glutaminase kidney isoform, mitochondrial in samples from serum, plasma, tissue homogenates and other biological fluids.

Human GLS/ Glutaminase kidney isoform, mitochondrial ELISA Kit

E1023Hu 1 Kit
EUR 605

Human GLS(Glutaminase kidney isoform, mitochondrial) ELISA Kit

EH1742 96T
EUR 567.6
  • Detection range: 0.312-20 ng/ml
  • Uniprot ID: O94925
  • Alias: GLS/Glutaminase kidney isoform, mitochondrial/GLS/K-glutaminase/L-glutamine amidohydrolase/GLS1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Recombinant Human Glutaminase kidney isoform, GST, E.coli-100ug

QP6095-ec-100ug 100ug
EUR 571

Recombinant Human Glutaminase kidney isoform, GST, E.coli-10ug

QP6095-ec-10ug 10ug
EUR 272

Recombinant Human Glutaminase kidney isoform, GST, E.coli-1mg

QP6095-ec-1mg 1mg
EUR 2303

Recombinant Human Glutaminase kidney isoform, GST, E.coli-200ug

QP6095-ec-200ug 200ug
EUR 898

Recombinant Human Glutaminase kidney isoform, GST, E.coli-500ug

QP6095-ec-500ug 500ug
EUR 1514

Recombinant Human Glutaminase kidney isoform, GST, E.coli-50ug

QP6095-ec-50ug 50ug
EUR 362

Human Glutaminase liver isoform, mitochondrial (GLS2)

1-CSB-EP887058HU
  • EUR 505.00
  • EUR 265.00
  • EUR 1827.00
  • EUR 766.00
  • EUR 1218.00
  • EUR 335.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 80.7 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Glutaminase liver isoform, mitochondrial(GLS2) expressed in E.coli

Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody

20-abx126919
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul
  • Shipped within 5-10 working days.

Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody

abx032915-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody

abx032915-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody

20-abx311936
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody (HRP)

20-abx311937
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody (FITC)

20-abx311938
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody (Biotin)

20-abx311939
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Gls2 ELISA Kit| Rat Glutaminase liver isoform, mitochondrial EL

EF018742 96 Tests
EUR 689

Human Glutaminase liver isoform, mitochondrial, GLS2 ELISA KIT

ELI-20970h 96 Tests
EUR 824

Mouse Glutaminase liver isoform, mitochondrial, Gls2 ELISA KIT

ELI-27365m 96 Tests
EUR 865

Gls2 ELISA Kit| Mouse Glutaminase liver isoform, mitochondrial

EF015050 96 Tests
EUR 689

Human Glutaminase liver isoform, mitochondrial (GLS2) ELISA Kit

abx392226-96tests 96 tests
EUR 911
  • Shipped within 5-12 working days.

Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-100ug

QP8210-ec-100ug 100ug
EUR 571

Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-10ug

QP8210-ec-10ug 10ug
EUR 272

Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-1mg

QP8210-ec-1mg 1mg
EUR 2303

Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-200ug

QP8210-ec-200ug 200ug
EUR 898

Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-500ug

QP8210-ec-500ug 500ug
EUR 1514

Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-50ug

QP8210-ec-50ug 50ug
EUR 362

Recombinant Glutaminase (GLS)

4-RPJ026Hu01
  • EUR 440.48
  • EUR 221.00
  • EUR 1376.80
  • EUR 525.60
  • EUR 951.20
  • EUR 358.00
  • EUR 3292.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: O94925
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 38.1kDa
  • Isoelectric Point: Inquire
Description: Recombinant Human Glutaminase expressed in: E.coli

Recombinant Glutaminase (GLS)

4-RPJ026Mu01
  • EUR 476.32
  • EUR 230.00
  • EUR 1511.20
  • EUR 570.40
  • EUR 1040.80
  • EUR 382.00
  • EUR 3628.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: D3Z7P3
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 27.6kDa
  • Isoelectric Point: Inquire
Description: Recombinant Mouse Glutaminase expressed in: E.coli

Recombinant E.Coli Glutaminase 1

7-02857 5µg Ask for price

Recombinant E.Coli Glutaminase 1

7-02858 20µg Ask for price

Recombinant E.Coli Glutaminase 1

7-02859 1mg Ask for price

E.Coli Glutaminase 1 (Recombinant)

20-abx073219
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Glutaminase recombinant monoclonal antibody

A5125 100ul X 3
EUR 595
  • Comparisons between Mnoclonal, Polyclonal and Recombinant antibodies and their benefits: Regular monoclonal antibodies have higher purity, better specificity and less lot-to-lot variations than polyclonal antibodies. Recombinant antibodies, however,
  • Show more
Description: A recombinant monoclonal antibody from rabbit against human Glutaminase for WB, IHC, IF,ELISA

Recombinant Glutaminase 2 (GLS2)

4-RPJ025Mu01
  • EUR 485.28
  • EUR 233.00
  • EUR 1544.80
  • EUR 581.60
  • EUR 1063.20
  • EUR 388.00
  • EUR 3712.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q571F8
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 39.4kDa
  • Isoelectric Point: Inquire
Description: Recombinant Mouse Glutaminase 2 expressed in: E.coli

Recombinant Aspartate Glutamate Carrier Isoform 1, Mitochondrial (Agc1)

4-RPE462Hu01
  • EUR 413.60
  • EUR 214.00
  • EUR 1276.00
  • EUR 492.00
  • EUR 884.00
  • EUR 340.00
  • EUR 3040.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: O75746
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 31.1kDa
  • Isoelectric Point: 5.8
Description: Recombinant Human Aspartate Glutamate Carrier Isoform 1, Mitochondrial expressed in: E.coli

Rat Glutaminase (GLS) Protein

20-abx653578
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.

Glutaminase Antibody

49063-100ul 100ul
EUR 333

Glutaminase Antibody

49063-50ul 50ul
EUR 239

anti-glutaminase

YF-PA23783 50 ul
EUR 334
Description: Mouse polyclonal to glutaminase

Human Kidney mitochondrial carrier protein 1 (SLC25A30)

1-CSB-EP732909HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 48.5 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Kidney mitochondrial carrier protein 1(SLC25A30) expressed in E.coli

Rat Glutaminase (GLS) ELISA Kit

DLR-GLS-Ra-48T 48T
EUR 549
  • Should the Rat Glutaminase (GLS) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Glutaminase (GLS) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Glutaminase (GLS) ELISA Kit

DLR-GLS-Ra-96T 96T
EUR 718
  • Should the Rat Glutaminase (GLS) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Glutaminase (GLS) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Glutaminase (GLS) ELISA Kit

20-abx155589
  • EUR 7237.00
  • EUR 3855.00
  • EUR 895.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Rat Glutaminase (GLS) ELISA Kit

abx573176-96tests 96 tests
EUR 668
  • Shipped within 5-12 working days.

Rat Glutaminase (GLS) CLIA Kit

20-abx495597
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Please enquire.

Rat Glutaminase (GLS) ELISA Kit

RDR-GLS-Ra-48Tests 48 Tests
EUR 583

Rat Glutaminase (GLS) ELISA Kit

RDR-GLS-Ra-96Tests 96 Tests
EUR 811

Rat Glutaminase ELISA Kit (GLS)

RK03685 96 Tests
EUR 521

Rat Glutaminase (GLS) ELISA Kit

RD-GLS-Ra-48Tests 48 Tests
EUR 557

Rat Glutaminase (GLS) ELISA Kit

RD-GLS-Ra-96Tests 96 Tests
EUR 775

Rat Glutaminase (GLS) ELISA Kit

SEJ026Ra-10x96wellstestplate 10x96-wells test plate
EUR 5124.2
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Glutaminase (GLS) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Glutaminase (GLS) in serum, plasma, tissue homogenates and other biological fluids.

Rat Glutaminase (GLS) ELISA Kit

SEJ026Ra-1x48wellstestplate 1x48-wells test plate
EUR 509.64
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Glutaminase (GLS) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Glutaminase (GLS) in serum, plasma, tissue homogenates and other biological fluids.

Rat Glutaminase (GLS) ELISA Kit

SEJ026Ra-1x96wellstestplate 1x96-wells test plate
EUR 685.2
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Glutaminase (GLS) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Glutaminase (GLS) in serum, plasma, tissue homogenates and other biological fluids.

Rat Glutaminase (GLS) ELISA Kit

SEJ026Ra-5x96wellstestplate 5x96-wells test plate
EUR 2783.4
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Glutaminase (GLS) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Glutaminase (GLS) in serum, plasma, tissue homogenates and other biological fluids.

Rat Glutaminase (GLS) ELISA Kit

4-SEJ026Ra
  • EUR 5175.00
  • EUR 2734.00
  • EUR 686.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Glutaminase elisa. Alternative names of the recognized antigen: GLS1
  • Glutaminase kidney isoform, mitochondrial
  • K-glutaminase
  • L-glutamine amidohydrolase
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Glutaminase (GLS) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Rat Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-82556 1 Kit Ask for price

V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody

20-abx007167
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody

20-abx009560
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody

abx026311-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody

abx026311-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody

abx026703-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody

abx026703-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody

20-abx111150
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody

20-abx126835
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul
  • Shipped within 5-10 working days.

V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody

abx036994-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.

V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody

20-abx320185
  • EUR 300.00
  • EUR 244.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody

20-abx326012
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody

20-abx322247
  • EUR 300.00
  • EUR 244.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody

abx230717-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.

Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol)

RAT-5 1
EUR 1138

GLSA1 Glutaminase 1 E.Coli Recombinant Protein

PROTP77454 Regular: 20ug
EUR 317
Description: GLSA1 E.Coli Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 330 amino acids (1-310a.a.) and having a molecular mass of 35.0kDa. ;The GLSA1 is purified by proprietary chromatographic techniques.

Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-100ug

QP7723-ec-100ug 100ug
EUR 408

Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-10ug

QP7723-ec-10ug 10ug
EUR 200

Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-1mg

QP7723-ec-1mg 1mg
EUR 1632

Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-200ug

QP7723-ec-200ug 200ug
EUR 634

Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-500ug

QP7723-ec-500ug 500ug
EUR 1060

Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-50ug

QP7723-ec-50ug 50ug
EUR 263

Glutaminase Rabbit mAb

A11043-100ul 100 ul
EUR 410

Glutaminase Rabbit mAb

A11043-200ul 200 ul
EUR 571

Glutaminase Rabbit mAb

A11043-20ul 20 ul
EUR 221

Glutaminase Rabbit mAb

A11043-50ul 50 ul
EUR 287

Glutaminase (GLS) Antibody

20-abx002829
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

Glutaminase (GLS) Antibody

20-abx213860
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Glutaminase (GLS) Antibody

20-abx214112
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Glutaminase (GLS) Antibody

20-abx176643
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug
  • Please enquire.

Glutaminase (GLS) Antibody

20-abx176644
  • EUR 1302.00
  • EUR 620.00
  • 1 mg
  • 200 ug
  • Please enquire.

Glutaminase 1 Antibody

20-abx110559
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Glutaminase (GLS) Antibody

20-abx112760
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

Glutaminase (GLS) Antibody

20-abx129148
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Glutaminase (GLS) Antibody

20-abx129432
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Glutaminase (GLS) Antibody

20-abx172592
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug
  • Please enquire.

Glutaminase (GLS) Antibody

20-abx172593
  • EUR 913.00
  • EUR 467.00
  • 1 mg
  • 200 ug
  • Please enquire.

Genotyping of macrophage migration inhibitory issue (MIF) CATT repeat polymorphism by denaturing high-performance liquid chromatography (DHPLC)

Macrophage migration inhibitory issue (MIF) is a proinflammatory cytokine expressed in many various cell sorts and implicated within the pathogenesis of quite a few acute and continual inflammatory ailments. Variable Variety of Tandem Repeat (VNTR) CATT5-Eight at place -794 within the promoter of the MIF gene has been related to a number of human pathological situations. Completely different strategies for genotyping the CATT tetranucleotide repeats have been described. Right here, we report, for the primary time, the whole characterization of the CATT5-Eight repeat polymorphism utilizing solely the denaturing high-performance liquid chromatography (DHPLC) approach beneath partially denaturing situations.

This strategy, based mostly on a step-by-step DHPLC protocol, allowed the correct dedication of all of the homozygous and heterozygous genotypes in 350 DNA samples from management topics. The outcomes had been validated by comparability to DNA sequencing, and the DHPLC strategy was correct, delicate, and extremely reproducible. Knowledge from the present examine reveal that this methodology of research by DHPLC could characterize a strong and delicate various instrument for a speedy and environment friendly genotyping of quick tandem repeats presenting a restricted variety of alleles.

DNA Sequence Fragment Containing C to A Mutation as a Handy Mutation Commonplace for DHPLC Evaluation 

OBJECTIVE

Denaturing excessive efficiency liquid chromatography (DHPLC) is a excessive throughput strategy for screening DNA sequence variations. To evaluate oven calibration, cartridge efficiency, buffer composition and stability, the WAVE Low and Excessive Vary Mutation Requirements are employed to make sure reproducibility and accuracy of the chromatographic evaluation. The aim of this examine was to offer an economical do-it-yourself mutation commonplace for DHPLC evaluation.

METHODS

DHPLC was carried out to judge completely different elution temperatures of a 374 bp DNA fragment with C>A mutation at place of 59 to attain a peak profile just like the Low Mutation Commonplace. So as to confirm the reproducibility of the do-it-yourself mutation commonplace utilizing DHPLC, 15 completely different experiments had been carried out to check the do-it-yourself mutation commonplace, the WAVE Low Vary Mutation Commonplace with a optimistic DNA management pattern.

RESULTS

We recognized a comparable elution temperature and a peak profile with the WAVE Low Vary Mutation Commonplace.

CONCLUSIONS

This examine confirmed the reproducibility of the height profile of our do-it-yourself mutation commonplace in comparison with the Low Mutation Commonplace utilizing DHPLC evaluation.

Alagille Syndrome: A New Missense Mutation Detected by Complete-Exome Sequencing in a Case Beforehand Discovered to Be Destructive by DHPLC and MLPA.

Alagille syndrome (ALGS, MIM 118450) is an autosomal dominant, multisystem dysfunction with excessive variability. Two genes have been described: JAG1 and NOTCH2. The inhabitants prevalence is 1:70,000 based mostly on the presence of neonatal liver illness. The vast majority of instances (∼97%) are brought on by haploinsufficiency of the JAG1 gene on 20p11.2p12, both as a consequence of mutations or deletions on the locus. Lower than 1% of instances are brought on by mutations in NOTCH2.

Essentially the most extensively used strategies for mutational screening embrace denaturing high-performance liquid chromatography (DHPLC) and multiplex ligation-dependent probe amplification (MLPA). Very lately, whole-exome sequencing (WES) has change into technically possible as a result of current advances in next-generation sequencing applied sciences, subsequently providing new alternatives for mutations/genes identification. A proband and its household, damaging for the presence of mutations in JAG1 and NOTCH2 genes by neither DHPLC nor MLPA, had been analyzed by WES. A missense mutation, not beforehand described, in JAG1 gene was recognized. This outcome reveals an enchancment within the mutation detection fee as a consequence of novel sequencing know-how suggesting the robust must reanalyze all damaging instances.

DHPLC is a extremely delicate and speedy screening methodology to detect BRAF(V600E) mutation in papillary thyroid carcinoma.

The BRAF(V600E) mutation has been reported to happen in 30% to 80% of papillary thyroid carcinomas (PTCs). Though direct sequencing is the strategy mostly used to determine mutations, this method isn’t delicate sufficient to precisely detect low stage mutation. To find out the optimum diagnostic methodology for detecting the BRAF(V600E) mutation in PTC, we in contrast the diagnostic efficacy of 4 consultant detection strategies in formalin-fixed paraffin-embedded thyroid tissues obtained from 40 sufferers identified with PTC. To detect the BRAF(V600E) mutation, we amplified exon 15 of the BRAF gene and carried out mutational evaluation with direct sequencing, denaturing high-performance liquid chromatography (DHPLC), pyrosequencing and colorimetric assay.

The BRAF mutation was detected in 33 instances (82.5%) by DHPLC, 23 instances (57.5%) by direct sequencing, 22 instances (55.0%) by pyrosequencing, and 37 instances (92.5%) by colorimetric assay. The sensitivity, damaging predictive worth and accuracy of DHPLC had been 100%. The specificity and optimistic predictive values for DHPLC, direct sequencing and pyrosequencing had been 100%, and for colorimetric assay they had been 14.3% and 83.8%, respectively. The kappa worth for DHPLC was an ideal 1.0, which was superior to the opposite strategies. In conclusion, DHPLC is a delicate, particular and correct methodology for detecting the BRAF(V600E) mutation, particularly low stage mutation, in PTC.

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