Application of DHPLC screening TGFBR-3 gene in Chinese women with idiopathic premature

Software of DHPLC screening TGFBR-Three gene in Chinese language girls with idiopathic untimely ovarian failure

OBJECTIVE

To guage scientific worth of denaturing excessive efficiency liquid chromatography (DHPLC) utilized in detecting remodeling development issue beta receptor 3 (TGFBR-3) exons 11 and 12 polymorphism in girls with idiopathic untimely ovarian failure (POF).

METHODS

From Feb. 2009 to Dec. 2011, 110 sufferers with idiopathic POF present process therapy at Shenzhen Maternal & Youngster Well being Institute affiliated to Southern Medical College had been enrolled as POF group on this examine. At the moment, 110 girls beneath 40 years outdated with regular hormonal stage and menstrual cycles as management group. The exons 11 and 12 of TGFBR-3 gene polymorphism had been screened through the use of DHPLC, and outcomes of DNA sequencing was as golden commonplace.

Some associated indexes had been calculated, equivalent to sensitivity, specificity, false damaging worth, false optimistic worth, Youden index, optimistic predictive worth, and damaging predictive worth. On the similar time, 20% of the examined specimens had been chosen randomly and detected by DHPLC once more. The worth of Kappa index had been calculated by evaluating the outcomes between the primary and second DHPLC evaluation.

RESULTS

The exon 11 of TGFBR-Three weren’t recognized gene polymorphism and two nucleotide polymorphisms had been recognized in exon 12. For 2022 T/C polymorphism, the frequencies of CC with 0.9% (1/110), TC with 22.7% (25/110), TT with 76.4% (84/110), C with 12.3% (27/220) and T with 87.7% (193/220) in POF group had been considerably completely different from CC with 0, TC with 9.1% (10/110) and TT with 90.9% (100/110), C with 4.5% (10/220) and T with 95.5% (210/220) in management group (all P<0.05).

Allelic and genotypic frequencies of 2161-75 C/T weren’t differed considerably between the 2 teams (all P>0.05). As DNA sequencing as golden commonplace, DHPLC confirmed that the sensitivity was 100%, specificity was 97.9%, Youden index was 97.9%, optimistic predictive worth was 96.3%, damaging predictive worth was 100%, and Kappa index was 0.888 (P<0.05).

CONCLUSIONS

DHPLC evaluation is greater validity, reliability and practicability methodology in detecting TGFBR-3 polymorphism in idiopathic untimely ovarian failure.

Recombinant Human Glutaminase kidney isoform, GST, E.coli-1mg
QP6095-ec-1mg	EnQuireBio	1mg	EUR 2763.6

Recombinant Human Glutaminase kidney isoform, GST, E.coli-10ug
QP6095-ec-10ug	EnQuireBio	10ug	EUR 326.4

Recombinant Human Glutaminase kidney isoform, GST, E.coli-50ug
QP6095-ec-50ug	EnQuireBio	50ug	EUR 434.4

Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody
20-abx311936	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody
abx032915-400ul	Abbexa	400 ul	EUR 627.6

Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody
abx032915-80l	Abbexa	80 µl	EUR 343.2

Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody
20-abx126919	Abbexa	EUR 493.20 EUR 710.40	100 ul 200 ul

Recombinant Human Glutaminase kidney isoform, GST, E.coli-100ug
QP6095-ec-100ug	EnQuireBio	100ug	EUR 685.2

Recombinant Human Glutaminase kidney isoform, GST, E.coli-200ug
QP6095-ec-200ug	EnQuireBio	200ug	EUR 1077.6

Recombinant Human Glutaminase kidney isoform, GST, E.coli-500ug
QP6095-ec-500ug	EnQuireBio	500ug	EUR 1816.8

Gls2 ELISA Kit| Rat Glutaminase liver isoform, mitochondrial EL
EF018742	Lifescience Market	96 Tests	EUR 826.8

Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody (HRP)
20-abx311937	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody (FITC)
20-abx311938	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody (Biotin)
20-abx311939	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Human Glutaminase liver isoform, mitochondrial (GLS2) ELISA Kit
abx392226-96tests	Abbexa	96 tests	EUR 1093.2

Mouse Glutaminase liver isoform, mitochondrial, Gls2 ELISA KIT
ELI-27365m	Lifescience Market	96 Tests	EUR 1038

Gls2 ELISA Kit| Mouse Glutaminase liver isoform, mitochondrial
EF015050	Lifescience Market	96 Tests	EUR 826.8

Human Glutaminase liver isoform, mitochondrial, GLS2 ELISA KIT
ELI-20970h	Lifescience Market	96 Tests	EUR 988.8

Recombinant Aspartate Glutamate Carrier Isoform 1, Mitochondrial (Agc1)
4-RPE462Hu01	Cloud-Clone	EUR 496.32 EUR 256.80 EUR 1531.20 EUR 590.40 EUR 1060.80 EUR 408.00 EUR 3648.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Aspartate Glutamate Carrier Isoform 1, Mitochondrial expressed in: E.coli

Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-1mg
QP8210-ec-1mg	EnQuireBio	1mg	EUR 2763.6

Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-100ug
QP8210-ec-100ug	EnQuireBio	100ug	EUR 685.2

Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-10ug
QP8210-ec-10ug	EnQuireBio	10ug	EUR 326.4

Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-200ug
QP8210-ec-200ug	EnQuireBio	200ug	EUR 1077.6

Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-500ug
QP8210-ec-500ug	EnQuireBio	500ug	EUR 1816.8

Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-50ug
QP8210-ec-50ug	EnQuireBio	50ug	EUR 434.4

Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-1mg
QP7723-ec-1mg	EnQuireBio	1mg	EUR 1958.4

Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-100ug
QP7723-ec-100ug	EnQuireBio	100ug	EUR 489.6

Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-10ug
QP7723-ec-10ug	EnQuireBio	10ug	EUR 240

Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-200ug
QP7723-ec-200ug	EnQuireBio	200ug	EUR 760.8

Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-500ug
QP7723-ec-500ug	EnQuireBio	500ug	EUR 1272

Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-50ug
QP7723-ec-50ug	EnQuireBio	50ug	EUR 315.6

Recombinant Glutaminase (GLS)
4-RPJ026Hu01	Cloud-Clone	EUR 528.58 EUR 265.20 EUR 1652.16 EUR 630.72 EUR 1141.44 EUR 429.60 EUR 3950.40	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Glutaminase expressed in: E.coli

Recombinant Glutaminase (GLS)
4-RPJ026Mu01	Cloud-Clone	EUR 571.58 EUR 276.00 EUR 1813.44 EUR 684.48 EUR 1248.96 EUR 458.40 EUR 4353.60	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Glutaminase expressed in: E.coli

E.Coli Glutaminase 1 (Recombinant)
20-abx073219	Abbexa	EUR 4101.60 EUR 393.60 EUR 276.00	1 mg 20 ug 5 ug

Recombinant E.Coli Glutaminase 1
7-02857	CHI Scientific	5µg	Ask for price

Recombinant E.Coli Glutaminase 1
7-02858	CHI Scientific	20µg	Ask for price

Recombinant E.Coli Glutaminase 1
7-02859	CHI Scientific	1mg	Ask for price

Recombinant Glutaminase 2 (GLS2)
4-RPJ025Mu01	Cloud-Clone	EUR 582.34 EUR 279.60 EUR 1853.76 EUR 697.92 EUR 1275.84 EUR 465.60 EUR 4454.40	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Glutaminase 2 expressed in: E.coli

Glutaminase recombinant monoclonal antibody
A5125	Bimake	100ul X 3	EUR 714
Description: A recombinant monoclonal antibody from rabbit against human Glutaminase for WB, IHC, IF,ELISA

Porcine ATP synthase subunit alpha liver isoform, mitochondrial,
ELI-48884p	Lifescience Market	96 Tests	EUR 1113.6

Rat Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E02C1969-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E02C1969-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E02C1969-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E02C1970-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E02C1970-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E02C1970-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Cox4i1/ Cytochrome c oxidase subunit 4 isoform 1, mitochondrial ELISA Kit
E0222Ra	Sunlong	1 Kit	EUR 775.2

Rabbit Cytochrome c oxidase subunit 4 isoform 1, mitochondrial,
ELI-47166Ra	Lifescience Market	96 Tests	EUR 1113.6

Recombinant Aeromonas Salmonicida Glutaminase (aa 1-306)
VAng-Lsx1092-1mgEcoli	Creative Biolabs	1 mg (E. coli)	EUR 5212.8
Description: Aeromonas Salmonicida Glutaminase (aa 1-306), recombinant protein.

Recombinant Aeromonas Salmonicida Glutaminase (aa 1-306)
VAng-Lsx1092-500gEcoli	Creative Biolabs	500 µg (E. coli)	EUR 3528
Description: Aeromonas Salmonicida Glutaminase (aa 1-306), recombinant protein.

Recombinant Aeromonas Salmonicida Glutaminase (aa 1-306)
VAng-Lsx1092-50gEcoli	Creative Biolabs	50 µg (E. coli)	EUR 2404.8
Description: Aeromonas Salmonicida Glutaminase (aa 1-306), recombinant protein.

GLSA1 Glutaminase 1 E.Coli Recombinant Protein
PROTP77454	BosterBio	Regular: 20ug	EUR 380.4
Description: GLSA1 E.Coli Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 330 amino acids (1-310a.a.) and having a molecular mass of 35.0kDa. ;The GLSA1 is purified by proprietary chromatographic techniques.

Porcine Cytochrome c oxidase subunit 4 isoform 1, mitochondrial,
ELI-33879p	Lifescience Market	96 Tests	EUR 1113.6

Human Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (COX4I1)
1-CSB-EP005832HU	Cusabio	EUR 456.00 EUR 256.80 EUR 1570.80 EUR 672.00 EUR 1047.60 EUR 314.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) expressed in E.coli

Human Kidney mitochondrial carrier protein 1 (SLC25A30)
1-CSB-EP732909HU	Cusabio	EUR 456.00 EUR 256.80 EUR 1570.80 EUR 672.00 EUR 1047.60 EUR 314.40	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Kidney mitochondrial carrier protein 1(SLC25A30) expressed in E.coli

Cytochrome C Oxidase Subunit 4 Isoform 1, Mitochondrial (COX41) Antibody
20-abx013969	Abbexa	EUR 376.80 EUR 117.60 EUR 477.60 EUR 594.00	100 ug 10 ug 200 ug 300 µg

Cytochrome C Oxidase Subunit 4 Isoform 1, Mitochondrial (COX4-1) Antibody
20-abx134040	Abbexa	EUR 427.20 EUR 644.40 EUR 260.40	100 ul 200 ul 30 ul

Cytochrome C Oxidase Subunit 4 Isoform 1, Mitochondrial (COX4-1) Antibody
20-abx134041	Abbexa	EUR 427.20 EUR 644.40 EUR 260.40	100 ul 200 ul 30 ul

Cytochrome C Oxidase Subunit 4 Isoform 1, Mitochondrial (COX-4) Antibody
abx448541-100ug	Abbexa	100 ug	EUR 627.6

Cytochrome C Oxidase Subunit 4 Isoform 1, Mitochondrial (COX-4) Antibody (ALP)
abx445404-100ug	Abbexa	100 ug	EUR 693.6

Cytochrome C Oxidase Subunit 4 Isoform 1, Mitochondrial (COX-4) Antibody (APC)
abx445405-100ug	Abbexa	100 ug	EUR 693.6

Cytochrome C Oxidase Subunit 4 Isoform 1, Mitochondrial (COX-4) Antibody (RPE)
abx445411-100ug	Abbexa	100 ug	EUR 693.6

Dog Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E08C1969-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E08C1969-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E08C1969-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E08C1970-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E08C1970-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E08C1970-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E07C1969-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E07C1969-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E07C1969-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E07C1970-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E07C1970-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E07C1970-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

ELISA kit for Mouse Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
EK2537	SAB	96 tests	EUR 804
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Cytochrome c oxidase subunit 4 isoform 1, mitochondrial in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Human Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
EK2538	SAB	96 tests	EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Cytochrome c oxidase subunit 4 isoform 1, mitochondrial in samples from serum, plasma, tissue homogenates and other biological fluids.

Cytochrome c oxidase subunit 4 isoform 1, mitochondrial Polyclonal Antibody
42116-100ul	SAB	100ul	EUR 399.6

Cytochrome C Oxidase Subunit 4 Isoform 1, Mitochondrial (COX-4) Antibody (PerCP)
abx445410-100ug	Abbexa	100 ug	EUR 693.6

Human COX4I1/ Cytochrome c oxidase subunit 4 isoform 1, mitochondrial ELISA Kit
E0540Hu	Sunlong	1 Kit	EUR 726

Mouse Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E03C1969-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E03C1969-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E03C1969-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E03C1970-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E03C1970-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E03C1970-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Cox4i1/ Cytochrome c oxidase subunit 4 isoform 1, mitochondrial ELISA Kit
E0312Mo	Sunlong	1 Kit	EUR 758.4

Human Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E01C1969-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E01C1969-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Human Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E01C1969-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Human Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E01C1970-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E01C1970-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Human Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E01C1970-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Human Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E06C1969-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E06C1969-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E06C1969-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E06C1970-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E06C1970-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E06C1970-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human COX4I1(Cytochrome c oxidase subunit 4 isoform 1, mitochondrial) ELISA Kit
EH1128	FN Test	96T	EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Recombinant human Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial
P2177	FN Test	100ug	Ask for price
Description: Recombinant protein for human Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial

Recombinant purified Glutaminase 1 (ybaS, E. Coli, his-tag)
RP-1622	Alpha Diagnostics	20 ug	EUR 416.4

Rabbit Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E04C1969-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E04C1969-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E04C1969-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E04C1970-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E04C1970-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) ELISA kit
E04C1970-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Cytochrome c oxidase subunit 4 isoform 2, mitochondrial(COX4I2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E09C1969-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E09C1969-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) ELISA kit
E09C1969-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey Cytochrome c oxidase subunit 4 isoform 1, mitochondrial(COX4I1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Genotyping of macrophage migration inhibitory issue (MIF) CATT repeat polymorphism by denaturing high-performance liquid chromatography (DHPLC)

Macrophage migration inhibitory issue (MIF) is a proinflammatory cytokine expressed in many various cell sorts and implicated within the pathogenesis of quite a few acute and continual inflammatory ailments. Variable Variety of Tandem Repeat (VNTR) CATT5-Eight at place -794 within the promoter of the MIF gene has been related to a number of human pathological situations. Completely different strategies for genotyping the CATT tetranucleotide repeats have been described. Right here, we report, for the primary time, the whole characterization of the CATT5-Eight repeat polymorphism utilizing solely the denaturing high-performance liquid chromatography (DHPLC) approach beneath partially denaturing situations.

This strategy, based mostly on a step-by-step DHPLC protocol, allowed the correct dedication of all of the homozygous and heterozygous genotypes in 350 DNA samples from management topics. The outcomes had been validated by comparability to DNA sequencing, and the DHPLC strategy was correct, delicate, and extremely reproducible. Knowledge from the present examine reveal that this methodology of research by DHPLC could characterize a strong and delicate various instrument for a speedy and environment friendly genotyping of quick tandem repeats presenting a restricted variety of alleles.

DNA Sequence Fragment Containing C to A Mutation as a Handy Mutation Commonplace for DHPLC Evaluation 

OBJECTIVE

Denaturing excessive efficiency liquid chromatography (DHPLC) is a excessive throughput strategy for screening DNA sequence variations. To evaluate oven calibration, cartridge efficiency, buffer composition and stability, the WAVE Low and Excessive Vary Mutation Requirements are employed to make sure reproducibility and accuracy of the chromatographic evaluation. The aim of this examine was to offer an economical do-it-yourself mutation commonplace for DHPLC evaluation.

METHODS

DHPLC was carried out to judge completely different elution temperatures of a 374 bp DNA fragment with C>A mutation at place of 59 to attain a peak profile just like the Low Mutation Commonplace. So as to confirm the reproducibility of the do-it-yourself mutation commonplace utilizing DHPLC, 15 completely different experiments had been carried out to check the do-it-yourself mutation commonplace, the WAVE Low Vary Mutation Commonplace with a optimistic DNA management pattern.

RESULTS

We recognized a comparable elution temperature and a peak profile with the WAVE Low Vary Mutation Commonplace.

CONCLUSIONS

This examine confirmed the reproducibility of the height profile of our do-it-yourself mutation commonplace in comparison with the Low Mutation Commonplace utilizing DHPLC evaluation.

Alagille Syndrome: A New Missense Mutation Detected by Complete-Exome Sequencing in a Case Beforehand Discovered to Be Destructive by DHPLC and MLPA.

Alagille syndrome (ALGS, MIM 118450) is an autosomal dominant, multisystem dysfunction with excessive variability. Two genes have been described: JAG1 and NOTCH2. The inhabitants prevalence is 1:70,000 based mostly on the presence of neonatal liver illness. The vast majority of instances (∼97%) are brought on by haploinsufficiency of the JAG1 gene on 20p11.2p12, both as a consequence of mutations or deletions on the locus. Lower than 1% of instances are brought on by mutations in NOTCH2.

Essentially the most extensively used strategies for mutational screening embrace denaturing high-performance liquid chromatography (DHPLC) and multiplex ligation-dependent probe amplification (MLPA). Very lately, whole-exome sequencing (WES) has change into technically possible as a result of current advances in next-generation sequencing applied sciences, subsequently providing new alternatives for mutations/genes identification. A proband and its household, damaging for the presence of mutations in JAG1 and NOTCH2 genes by neither DHPLC nor MLPA, had been analyzed by WES. A missense mutation, not beforehand described, in JAG1 gene was recognized. This outcome reveals an enchancment within the mutation detection fee as a consequence of novel sequencing know-how suggesting the robust must reanalyze all damaging instances.

DHPLC is a extremely delicate and speedy screening methodology to detect BRAF(V600E) mutation in papillary thyroid carcinoma.

The BRAF(V600E) mutation has been reported to happen in 30% to 80% of papillary thyroid carcinomas (PTCs). Though direct sequencing is the strategy mostly used to determine mutations, this method isn’t delicate sufficient to precisely detect low stage mutation. To find out the optimum diagnostic methodology for detecting the BRAF(V600E) mutation in PTC, we in contrast the diagnostic efficacy of 4 consultant detection strategies in formalin-fixed paraffin-embedded thyroid tissues obtained from 40 sufferers identified with PTC. To detect the BRAF(V600E) mutation, we amplified exon 15 of the BRAF gene and carried out mutational evaluation with direct sequencing, denaturing high-performance liquid chromatography (DHPLC), pyrosequencing and colorimetric assay.

The BRAF mutation was detected in 33 instances (82.5%) by DHPLC, 23 instances (57.5%) by direct sequencing, 22 instances (55.0%) by pyrosequencing, and 37 instances (92.5%) by colorimetric assay. The sensitivity, damaging predictive worth and accuracy of DHPLC had been 100%. The specificity and optimistic predictive values for DHPLC, direct sequencing and pyrosequencing had been 100%, and for colorimetric assay they had been 14.3% and 83.8%, respectively. The kappa worth for DHPLC was an ideal 1.0, which was superior to the opposite strategies. In conclusion, DHPLC is a delicate, particular and correct methodology for detecting the BRAF(V600E) mutation, particularly low stage mutation, in PTC.