Software of DHPLC screening TGFBR-Three gene in Chinese language girls with idiopathic untimely ovarian failure
OBJECTIVE
To guage scientific worth of denaturing excessive efficiency liquid chromatography (DHPLC) utilized in detecting remodeling development issue beta receptor 3 (TGFBR-3) exons 11 and 12 polymorphism in girls with idiopathic untimely ovarian failure (POF).
METHODS
From Feb. 2009 to Dec. 2011, 110 sufferers with idiopathic POF present process therapy at Shenzhen Maternal & Youngster Well being Institute affiliated to Southern Medical College had been enrolled as POF group on this examine. At the moment, 110 girls beneath 40 years outdated with regular hormonal stage and menstrual cycles as management group. The exons 11 and 12 of TGFBR-3 gene polymorphism had been screened through the use of DHPLC, and outcomes of DNA sequencing was as golden commonplace.
Some associated indexes had been calculated, equivalent to sensitivity, specificity, false damaging worth, false optimistic worth, Youden index, optimistic predictive worth, and damaging predictive worth. On the similar time, 20% of the examined specimens had been chosen randomly and detected by DHPLC once more. The worth of Kappa index had been calculated by evaluating the outcomes between the primary and second DHPLC evaluation.
RESULTS
The exon 11 of TGFBR-Three weren’t recognized gene polymorphism and two nucleotide polymorphisms had been recognized in exon 12. For 2022 T/C polymorphism, the frequencies of CC with 0.9% (1/110), TC with 22.7% (25/110), TT with 76.4% (84/110), C with 12.3% (27/220) and T with 87.7% (193/220) in POF group had been considerably completely different from CC with 0, TC with 9.1% (10/110) and TT with 90.9% (100/110), C with 4.5% (10/220) and T with 95.5% (210/220) in management group (all P<0.05).
Allelic and genotypic frequencies of 2161-75 C/T weren’t differed considerably between the 2 teams (all P>0.05). As DNA sequencing as golden commonplace, DHPLC confirmed that the sensitivity was 100%, specificity was 97.9%, Youden index was 97.9%, optimistic predictive worth was 96.3%, damaging predictive worth was 100%, and Kappa index was 0.888 (P<0.05).
CONCLUSIONS
DHPLC evaluation is greater validity, reliability and practicability methodology in detecting TGFBR-3 polymorphism in idiopathic untimely ovarian failure.

des-testbed
Recombinant Human Glutaminase kidney isoform, GST, E.coli-10ug |
QP6095-ec-10ug |
EnQuireBio |
10ug |
EUR 326.4 |
Recombinant Human Glutaminase kidney isoform, GST, E.coli-1mg |
QP6095-ec-1mg |
EnQuireBio |
1mg |
EUR 2763.6 |
Recombinant Human Glutaminase kidney isoform, GST, E.coli-200ug |
QP6095-ec-200ug |
EnQuireBio |
200ug |
EUR 1077.6 |
Recombinant Human Glutaminase kidney isoform, GST, E.coli-500ug |
QP6095-ec-500ug |
EnQuireBio |
500ug |
EUR 1816.8 |
Recombinant Human Glutaminase kidney isoform, GST, E.coli-50ug |
QP6095-ec-50ug |
EnQuireBio |
50ug |
EUR 434.4 |
Human Glutaminase liver isoform, mitochondrial (GLS2) |
1-CSB-EP887058HU |
Cusabio |
-
EUR 606.00
-
EUR 318.00
-
EUR 2192.40
-
EUR 919.20
-
EUR 1461.60
-
EUR 402.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Human Glutaminase liver isoform, mitochondrial(GLS2) expressed in E.coli |
Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody |
20-abx126919 |
Abbexa |
|
|
|
Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody |
abx032915-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody |
abx032915-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody |
20-abx311936 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody (HRP) |
20-abx311937 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody (FITC) |
20-abx311938 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Glutaminase Liver Isoform, Mitochondrial (GLS2) Antibody (Biotin) |
20-abx311939 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Gls2 ELISA Kit| Rat Glutaminase liver isoform, mitochondrial EL |
EF018742 |
Lifescience Market |
96 Tests |
EUR 826.8 |
Human Glutaminase liver isoform, mitochondrial (GLS2) ELISA Kit |
abx392226-96tests |
Abbexa |
96 tests |
EUR 1093.2 |
|
Gls2 ELISA Kit| Mouse Glutaminase liver isoform, mitochondrial |
EF015050 |
Lifescience Market |
96 Tests |
EUR 826.8 |
Mouse Glutaminase liver isoform, mitochondrial, Gls2 ELISA KIT |
ELI-27365m |
Lifescience Market |
96 Tests |
EUR 1038 |
Human Glutaminase liver isoform, mitochondrial, GLS2 ELISA KIT |
ELI-20970h |
Lifescience Market |
96 Tests |
EUR 988.8 |
Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-100ug |
QP8210-ec-100ug |
EnQuireBio |
100ug |
EUR 685.2 |
Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-10ug |
QP8210-ec-10ug |
EnQuireBio |
10ug |
EUR 326.4 |
Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-1mg |
QP8210-ec-1mg |
EnQuireBio |
1mg |
EUR 2763.6 |
Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-200ug |
QP8210-ec-200ug |
EnQuireBio |
200ug |
EUR 1077.6 |
Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-500ug |
QP8210-ec-500ug |
EnQuireBio |
500ug |
EUR 1816.8 |
Recombinant Human Glutaminase liver isoform, His-SUMO, E.coli-50ug |
QP8210-ec-50ug |
EnQuireBio |
50ug |
EUR 434.4 |
Recombinant Glutaminase (GLS) |
4-RPJ026Hu01 |
Cloud-Clone |
-
EUR 528.58
-
EUR 265.20
-
EUR 1652.16
-
EUR 630.72
-
EUR 1141.44
-
EUR 429.60
-
EUR 3950.40
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Human Glutaminase expressed in: E.coli |
Recombinant Glutaminase (GLS) |
4-RPJ026Mu01 |
Cloud-Clone |
-
EUR 571.58
-
EUR 276.00
-
EUR 1813.44
-
EUR 684.48
-
EUR 1248.96
-
EUR 458.40
-
EUR 4353.60
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Mouse Glutaminase expressed in: E.coli |
Recombinant E.Coli Glutaminase 1 |
7-02857 |
CHI Scientific |
5µg |
Ask for price |
Recombinant E.Coli Glutaminase 1 |
7-02858 |
CHI Scientific |
20µg |
Ask for price |
Recombinant E.Coli Glutaminase 1 |
7-02859 |
CHI Scientific |
1mg |
Ask for price |
E.Coli Glutaminase 1 (Recombinant) |
20-abx073219 |
Abbexa |
-
EUR 4101.60
-
EUR 393.60
-
EUR 276.00
|
|
|
Glutaminase recombinant monoclonal antibody |
A5125 |
Bimake |
100ul X 3 |
EUR 714 |
|
Description: A recombinant monoclonal antibody from rabbit against human Glutaminase for WB, IHC, IF,ELISA |
Recombinant Glutaminase 2 (GLS2) |
4-RPJ025Mu01 |
Cloud-Clone |
-
EUR 582.34
-
EUR 279.60
-
EUR 1853.76
-
EUR 697.92
-
EUR 1275.84
-
EUR 465.60
-
EUR 4454.40
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Mouse Glutaminase 2 expressed in: E.coli |
Recombinant Aspartate Glutamate Carrier Isoform 1, Mitochondrial (Agc1) |
4-RPE462Hu01 |
Cloud-Clone |
-
EUR 496.32
-
EUR 256.80
-
EUR 1531.20
-
EUR 590.40
-
EUR 1060.80
-
EUR 408.00
-
EUR 3648.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Human Aspartate Glutamate Carrier Isoform 1, Mitochondrial expressed in: E.coli |
Rat Glutaminase (GLS) Protein |
20-abx653578 |
Abbexa |
-
EUR 693.60
-
EUR 309.60
-
EUR 2064.00
-
EUR 828.00
-
EUR 510.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Glutaminase Antibody |
DF13334 |
Affbiotech |
100ul |
EUR 420 |
Glutaminase Antibody |
49063-100ul |
SAB |
100ul |
EUR 399.6 |
Glutaminase Antibody |
49063-50ul |
SAB |
50ul |
EUR 286.8 |
anti-glutaminase |
YF-PA23783 |
Abfrontier |
50 ul |
EUR 400.8 |
Description: Mouse polyclonal to glutaminase |
Human Kidney mitochondrial carrier protein 1 (SLC25A30) |
1-CSB-EP732909HU |
Cusabio |
-
EUR 456.00
-
EUR 256.80
-
EUR 1570.80
-
EUR 672.00
-
EUR 1047.60
-
EUR 314.40
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Human Kidney mitochondrial carrier protein 1(SLC25A30) expressed in E.coli |
Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol) |
RAT-5 |
Alpha Diagnostics |
1 |
EUR 1365.6 |
Rat Glutaminase (GLS) ELISA Kit |
DLR-GLS-Ra-48T |
DL Develop |
48T |
EUR 658.8 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Glutaminase (GLS) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Rat Glutaminase (GLS) ELISA Kit |
DLR-GLS-Ra-96T |
DL Develop |
96T |
EUR 861.6 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Glutaminase (GLS) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Rat Glutaminase (GLS) ELISA Kit |
20-abx155589 |
Abbexa |
-
EUR 8684.40
-
EUR 4626.00
-
EUR 1074.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Rat Glutaminase (GLS) ELISA Kit |
abx573176-96tests |
Abbexa |
96 tests |
EUR 801.6 |
|
Rat Glutaminase (GLS) CLIA Kit |
20-abx495597 |
Abbexa |
-
EUR 10282.80
-
EUR 5472.00
-
EUR 1262.40
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Rat Glutaminase (GLS) ELISA Kit |
RD-GLS-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 668.4 |
Rat Glutaminase (GLS) ELISA Kit |
RD-GLS-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 930 |
Rat Glutaminase (GLS) ELISA Kit |
RDR-GLS-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 699.6 |
Rat Glutaminase (GLS) ELISA Kit |
RDR-GLS-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 973.2 |
Rat Glutaminase (GLS) ELISA Kit |
SEJ026Ra-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 6149.04 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Glutaminase (GLS) in serum, plasma, tissue homogenates and other biological fluids. |
Rat Glutaminase (GLS) ELISA Kit |
SEJ026Ra-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 611.57 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Glutaminase (GLS) in serum, plasma, tissue homogenates and other biological fluids. |
Rat Glutaminase (GLS) ELISA Kit |
SEJ026Ra-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 822.24 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Glutaminase (GLS) in serum, plasma, tissue homogenates and other biological fluids. |
Rat Glutaminase (GLS) ELISA Kit |
SEJ026Ra-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 3340.08 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Glutaminase (GLS) in serum, plasma, tissue homogenates and other biological fluids. |
Rat Glutaminase (GLS) ELISA Kit |
4-SEJ026Ra |
Cloud-Clone |
-
EUR 6210.00
-
EUR 3280.80
-
EUR 823.20
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Glutaminase (GLS) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. |
Rat Glutaminase ELISA Kit (GLS) |
RK03685 |
Abclonal |
96 Tests |
EUR 625.2 |
Rat Kidney PrimaCell2: Normal Kidney Epithelial Cells |
2-82556 |
CHI Scientific |
1 Kit |
Ask for price |
V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody |
20-abx126835 |
Abbexa |
|
|
|
V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody |
20-abx111150 |
Abbexa |
|
|
|
V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody |
20-abx007167 |
Abbexa |
-
EUR 493.20
-
EUR 710.40
-
EUR 218.40
-
EUR 376.80
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody |
20-abx009560 |
Abbexa |
-
EUR 360.00
-
EUR 526.80
-
EUR 226.80
|
|
|
V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody |
abx026311-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody |
abx026311-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody |
abx026703-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody |
abx026703-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody |
abx036994-100ug |
Abbexa |
100 ug |
EUR 469.2 |
|
V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody |
abx230717-100ug |
Abbexa |
100 ug |
EUR 610.8 |
|
V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody |
20-abx320185 |
Abbexa |
|
|
|
V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody |
20-abx322247 |
Abbexa |
|
|
|
V-Type Proton ATPase Subunit B, Kidney Isoform (ATP6V1B1) Antibody |
20-abx326012 |
Abbexa |
|
|
|
Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-100ug |
QP7723-ec-100ug |
EnQuireBio |
100ug |
EUR 489.6 |
Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-10ug |
QP7723-ec-10ug |
EnQuireBio |
10ug |
EUR 240 |
Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-1mg |
QP7723-ec-1mg |
EnQuireBio |
1mg |
EUR 1958.4 |
Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-200ug |
QP7723-ec-200ug |
EnQuireBio |
200ug |
EUR 760.8 |
Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-500ug |
QP7723-ec-500ug |
EnQuireBio |
500ug |
EUR 1272 |
Recombinant Human Kidney mitochondrial carrier protein 1, His-SUMO, E.coli-50ug |
QP7723-ec-50ug |
EnQuireBio |
50ug |
EUR 315.6 |
Glutaminase Conjugated Antibody |
C49063 |
SAB |
100ul |
EUR 476.4 |
Glutaminase Rabbit mAb |
A11043 |
Abclonal |
20 μL |
EUR 18 |
Glutaminase Rabbit mAb |
A11043-100ul |
Abclonal |
100 ul |
EUR 492 |
Glutaminase Rabbit mAb |
A11043-200ul |
Abclonal |
200 ul |
EUR 685.2 |
Glutaminase Rabbit mAb |
A11043-20ul |
Abclonal |
20 ul |
EUR 265.2 |
Glutaminase Rabbit mAb |
A11043-50ul |
Abclonal |
50 ul |
EUR 344.4 |
Glutaminase 1 Antibody |
20-abx110559 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Glutaminase (GLS) Antibody |
20-abx172592 |
Abbexa |
|
|
|
Glutaminase (GLS) Antibody |
20-abx172593 |
Abbexa |
|
|
|
Glutaminase (GLS) Antibody |
20-abx112760 |
Abbexa |
|
|
|
Glutaminase (GLS) Antibody |
20-abx129148 |
Abbexa |
-
EUR 510.00
-
EUR 159.60
-
EUR 1446.00
-
EUR 693.60
-
EUR 393.60
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Glutaminase (GLS) Antibody |
20-abx129432 |
Abbexa |
-
EUR 526.80
-
EUR 159.60
-
EUR 1479.60
-
EUR 710.40
-
EUR 393.60
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Glutaminase (GLS) Antibody |
20-abx002829 |
Abbexa |
-
EUR 493.20
-
EUR 710.40
-
EUR 218.40
-
EUR 376.80
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Glutaminase (GLS) Antibody |
abx034114-400ul |
Abbexa |
400 ul |
EUR 627.6 |
|
Glutaminase (GLS) Antibody |
abx034114-80l |
Abbexa |
80 µl |
EUR 343.2 |
|
Glutaminase (GLS) Antibody |
20-abx318516 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Glutaminase Assay Kit |
abx298831-100Assays |
Abbexa |
100 Assays |
EUR 661.2 |
|
Glutaminase (GLS) Antibody |
20-abx303988 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Glutaminase (GLS) Antibody |
20-abx176643 |
Abbexa |
|
|
|
Glutaminase (GLS) Antibody |
20-abx176644 |
Abbexa |
|
|
|
Glutaminase (GLS) Antibody |
20-abx213860 |
Abbexa |
|
|
|
Glutaminase (GLS) Antibody |
20-abx214112 |
Abbexa |
|
|
|
Anti-glutaminase (5C4) |
YF-MA10377 |
Abfrontier |
100 ug |
EUR 435.6 |
Description: Mouse monoclonal to glutaminase |
Anti-Glutaminase (6H1) |
YF-MA13261 |
Abfrontier |
100 ug |
EUR 435.6 |
Description: Mouse monoclonal to Glutaminase |
Anti-Glutaminase (5C9) |
YF-MA13262 |
Abfrontier |
200 ul |
EUR 435.6 |
Description: Mouse monoclonal to Glutaminase |
Anti-Glutaminase (5B7) |
YF-MA13263 |
Abfrontier |
100 ug |
EUR 435.6 |
Description: Mouse monoclonal to Glutaminase |
Anti-Glutaminase (6E12) |
YF-MA13264 |
Abfrontier |
100 ug |
EUR 435.6 |
Description: Mouse monoclonal to Glutaminase |
Glutaminase Antibody / GLS |
RQ6733 |
NSJ Bioreagents |
100ug |
EUR 419 |
Description: This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. |
Glutaminase Antibody / GLS |
RQ6734 |
NSJ Bioreagents |
100ug |
EUR 419 |
Description: This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. |
GLSA1 Glutaminase 1 E.Coli Recombinant Protein |
PROTP77454 |
BosterBio |
Regular: 20ug |
EUR 380.4 |
Description: GLSA1 E.Coli Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 330 amino acids (1-310a.a.) and having a molecular mass of 35.0kDa. ;The GLSA1 is purified by proprietary chromatographic techniques. |
ELISA kit for Rat GLS (Glutaminase) |
ELK7190 |
ELK Biotech |
1 plate of 96 wells |
EUR 518.4 |
|
Description: A sandwich ELISA kit for detection of Glutaminase from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
Rat Glutaminase 2 (GLS2) ELISA Kit |
abx391386-96tests |
Abbexa |
96 tests |
EUR 1093.2 |
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Genotyping of macrophage migration inhibitory issue (MIF) CATT repeat polymorphism by denaturing high-performance liquid chromatography (DHPLC)
Macrophage migration inhibitory issue (MIF) is a proinflammatory cytokine expressed in many various cell sorts and implicated within the pathogenesis of quite a few acute and continual inflammatory ailments. Variable Variety of Tandem Repeat (VNTR) CATT5-Eight at place -794 within the promoter of the MIF gene has been related to a number of human pathological situations. Completely different strategies for genotyping the CATT tetranucleotide repeats have been described. Right here, we report, for the primary time, the whole characterization of the CATT5-Eight repeat polymorphism utilizing solely the denaturing high-performance liquid chromatography (DHPLC) approach beneath partially denaturing situations.
This strategy, based mostly on a step-by-step DHPLC protocol, allowed the correct dedication of all of the homozygous and heterozygous genotypes in 350 DNA samples from management topics. The outcomes had been validated by comparability to DNA sequencing, and the DHPLC strategy was correct, delicate, and extremely reproducible. Knowledge from the present examine reveal that this methodology of research by DHPLC could characterize a strong and delicate various instrument for a speedy and environment friendly genotyping of quick tandem repeats presenting a restricted variety of alleles.
DNA Sequence Fragment Containing C to A Mutation as a Handy Mutation Commonplace for DHPLC Evaluation
OBJECTIVE
Denaturing excessive efficiency liquid chromatography (DHPLC) is a excessive throughput strategy for screening DNA sequence variations. To evaluate oven calibration, cartridge efficiency, buffer composition and stability, the WAVE Low and Excessive Vary Mutation Requirements are employed to make sure reproducibility and accuracy of the chromatographic evaluation. The aim of this examine was to offer an economical do-it-yourself mutation commonplace for DHPLC evaluation.
METHODS
DHPLC was carried out to judge completely different elution temperatures of a 374 bp DNA fragment with C>A mutation at place of 59 to attain a peak profile just like the Low Mutation Commonplace. So as to confirm the reproducibility of the do-it-yourself mutation commonplace utilizing DHPLC, 15 completely different experiments had been carried out to check the do-it-yourself mutation commonplace, the WAVE Low Vary Mutation Commonplace with a optimistic DNA management pattern.
RESULTS
We recognized a comparable elution temperature and a peak profile with the WAVE Low Vary Mutation Commonplace.
CONCLUSIONS
This examine confirmed the reproducibility of the height profile of our do-it-yourself mutation commonplace in comparison with the Low Mutation Commonplace utilizing DHPLC evaluation.
Alagille Syndrome: A New Missense Mutation Detected by Complete-Exome Sequencing in a Case Beforehand Discovered to Be Destructive by DHPLC and MLPA.
Alagille syndrome (ALGS, MIM 118450) is an autosomal dominant, multisystem dysfunction with excessive variability. Two genes have been described: JAG1 and NOTCH2. The inhabitants prevalence is 1:70,000 based mostly on the presence of neonatal liver illness. The vast majority of instances (∼97%) are brought on by haploinsufficiency of the JAG1 gene on 20p11.2p12, both as a consequence of mutations or deletions on the locus. Lower than 1% of instances are brought on by mutations in NOTCH2.
Essentially the most extensively used strategies for mutational screening embrace denaturing high-performance liquid chromatography (DHPLC) and multiplex ligation-dependent probe amplification (MLPA). Very lately, whole-exome sequencing (WES) has change into technically possible as a result of current advances in next-generation sequencing applied sciences, subsequently providing new alternatives for mutations/genes identification. A proband and its household, damaging for the presence of mutations in JAG1 and NOTCH2 genes by neither DHPLC nor MLPA, had been analyzed by WES. A missense mutation, not beforehand described, in JAG1 gene was recognized. This outcome reveals an enchancment within the mutation detection fee as a consequence of novel sequencing know-how suggesting the robust must reanalyze all damaging instances.
DHPLC is a extremely delicate and speedy screening methodology to detect BRAF(V600E) mutation in papillary thyroid carcinoma.
The BRAF(V600E) mutation has been reported to happen in 30% to 80% of papillary thyroid carcinomas (PTCs). Though direct sequencing is the strategy mostly used to determine mutations, this method isn’t delicate sufficient to precisely detect low stage mutation. To find out the optimum diagnostic methodology for detecting the BRAF(V600E) mutation in PTC, we in contrast the diagnostic efficacy of 4 consultant detection strategies in formalin-fixed paraffin-embedded thyroid tissues obtained from 40 sufferers identified with PTC. To detect the BRAF(V600E) mutation, we amplified exon 15 of the BRAF gene and carried out mutational evaluation with direct sequencing, denaturing high-performance liquid chromatography (DHPLC), pyrosequencing and colorimetric assay.
The BRAF mutation was detected in 33 instances (82.5%) by DHPLC, 23 instances (57.5%) by direct sequencing, 22 instances (55.0%) by pyrosequencing, and 37 instances (92.5%) by colorimetric assay. The sensitivity, damaging predictive worth and accuracy of DHPLC had been 100%. The specificity and optimistic predictive values for DHPLC, direct sequencing and pyrosequencing had been 100%, and for colorimetric assay they had been 14.3% and 83.8%, respectively. The kappa worth for DHPLC was an ideal 1.0, which was superior to the opposite strategies. In conclusion, DHPLC is a delicate, particular and correct methodology for detecting the BRAF(V600E) mutation, particularly low stage mutation, in PTC.