DES-Testbed, Freie Universität Research Pig,Plant,plasmid,Polyclonal,Real-time Detection of mutations in gyrB using denaturing high performance liquid chromatography (

Detection of mutations in gyrB using denaturing high performance liquid chromatography (

June 22, 2021June 22, 2021 FelixFelix 0 Comments 8:04 am
des-testbed

Detection of mutations in gyrB using denaturing high performance liquid chromatography (DHPLC) among Salmonella enterica serovar Typhi and Paratyphi A.

 

Fluoroquinolone resistance is mediated by mutations in the quinolone-resistance determining region (QRDR) of the topoisomerase genes. Denaturing high performance liquid chromatography (DHPLC) was evaluated for detection of clinically important mutations in gyrB among Salmonella.

Salmonella Typhi and S. Paratyphi A characterised for mutation in QRDR of gyrA, parC and parE were studied for mutation in gyrB by DHPLC and validated by sequencing.

The DHPLC analysis was able to resolve the test mutant from isolates with wild type gyrB and distinguished mutants from other mutant by peak profile and shift in retention time. Three sequence variants were detected at codon 464, and a novel mutation Ser→Thr was also detected. gyrB mutation was associated with non classical quinolone resistance (NALS-CIPDS) in 34 isolates of S. Typhi only and was distinct from classical quinolone resistance associated with gyrA mutations (NALR-CIPDS).

des-testbed

des-testbed

Setup of a Protocol of Molecular Diagnosis of β-Thalassemia Mutations in Tunisia using Denaturing High-Performance Liquid Chromatography (DHPLC)

BACKGROUND

β-Thalassemia is one of the most prevalent worldwide autosomal recessive disorders. It presents a great molecular heterogeneity resulting from more than 200 causative mutations in the β-globin gene. In Tunisia, β-thalassemia represents the most prevalent monogenic hemoglobin disorder with 2.21% of carriers.

Efficient and reliable mutation-screening methods are essential in order to establish appropriate prevention programs for at risk couples. The aim of the present study is to develop an efficient method based on the denaturing high-performance liquid chromatography (DHPLC) in which the whole β-globin gene (HBB) is screened for mutations covering about 90% of the spectrum.

METHODS

We have performed the validation of a DHPLC assay for direct genotyping of 11 known β-thalassemia mutations in the Tunisian population.

RESULTS

DHPLC assay was established based on the analysis of 62 archival β-thalassemia samples previously genotyped then validated with full concordance on 50 tests with blind randomized samples previously genotyped with DNA sequencing and with 96% of consistency on 40 samples as a prospective study.

CONCLUSIONS

Compared to other genotyping techniques, the DHPLC method can meet the requirements of direct genotyping of known β-thalassemia mutations in Tunisia and to be applied as a powerful tool for the genetic screening of prenatal and postnatal individuals.

 

Recombinant Human Zyxin (ZYX)
MBS969633-002mgMammalianCell 0.02mg(Mammalian-Cell)
EUR 425

Recombinant Human Zyxin (ZYX)
MBS969633-01mgMammalianCell 0.1mg(Mammalian-Cell)
EUR 1015

Recombinant Human Zyxin (ZYX)
MBS969633-5x01mgMammalianCell 5x0.1mg(Mammalian-Cell)
EUR 4305

Recombinant Human Zyxin (ZYX)
RPC20585-100ug 100ug
EUR 1218.8

Recombinant Human Zyxin (ZYX)
RPC20585-20ug 20ug
EUR 484

Zyxin (ZYX) (NM_003461) Human Recombinant Protein
E45H36212M5 20 ug
EUR 785.42

Zyxin (ZYX) (NM_001010972) Human Recombinant Protein
E45H41710M5 20 ug
EUR 785.42

Zyxin (ZYX) (NM_001010972) Human Recombinant Protein
PROTQ15942 20ug
EUR 1249
Description: Recombinant protein of human zyxin (ZYX), transcript variant 2

Recombinant Zyxin (ZYX)
MBS2010365-001mg 0.01mg
EUR 175

Recombinant Zyxin (ZYX)
MBS2010365-005mg 0.05mg
EUR 310

Recombinant Zyxin (ZYX)
MBS2010365-01mg 0.1mg
EUR 470

Recombinant Zyxin (ZYX)
MBS2010365-02mg 0.2mg
EUR 575

Recombinant Zyxin (ZYX)
MBS2010365-05mg 0.5mg
EUR 1090

Recombinant Zyxin (ZYX)
MBS2011079-001mg 0.01mg
EUR 175

Recombinant Zyxin (ZYX)
MBS2011079-005mg 0.05mg
EUR 310

Recombinant Zyxin (ZYX)
MBS2011079-01mg 0.1mg
EUR 470

Recombinant Zyxin (ZYX)
MBS2011079-02mg 0.2mg
EUR 575

Recombinant Zyxin (ZYX)
MBS2011079-05mg 0.5mg
EUR 1090

Recombinant Zyxin (ZYX)
RPU44398-100ug 100ug
EUR 554.4

Recombinant Zyxin (ZYX)
RPU44398-1mg 1mg
EUR 2457

Recombinant Zyxin (ZYX)
RPU44398-50ug 50ug
EUR 445.5

Recombinant Zyxin (ZYX)
RPU44399-100ug 100ug
EUR 554.4

Recombinant Zyxin (ZYX)
RPU44399-1mg 1mg
EUR 2457

Recombinant Zyxin (ZYX)
RPU44399-50ug 50ug
EUR 445.5

Recombinant Zyxin (ZYX)
RPC235Hu01 10ug
EUR 180

Recombinant Zyxin (ZYX)
4-RPC235Hu01
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  • 5 mg
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  • 1 mg
Description: Recombinant Human Zyxin expressed in: E.coli

Recombinant Zyxin (ZYX)
RPC235Hu02 10ug
EUR 180

Recombinant Zyxin (ZYX)
4-RPC235Hu02
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Description: Recombinant Human Zyxin expressed in: E.coli

Recombinant Mouse Zyxin (Zyx)
MBS1384721-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1425

Recombinant Mouse Zyxin (Zyx)
MBS1384721-002mgEColi 0.02mg(E-Coli)
EUR 1120

Recombinant Mouse Zyxin (Zyx)
MBS1384721-002mgYeast 0.02mg(Yeast)
EUR 1185

Recombinant Mouse Zyxin (Zyx)
MBS1384721-01mgEColi 0.1mg(E-Coli)
EUR 1335

Recombinant Mouse Zyxin (Zyx)
MBS1384721-01mgYeast 0.1mg(Yeast)
EUR 1390

Human Zyxin (ZYX)
1-CSB-MP027165HU
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Description: Recombinant Human Zyxin(ZYX) expressed in Mammalian cell

Recombinant Xenopus laevis Zyxin (zyx), partial
MBS1111145-INQUIRE INQUIRE Ask for price

Recombinant Xenopus tropicalis Zyxin (zyx), partial
MBS1454062-INQUIRE INQUIRE Ask for price

Human Zyxin (ZYX) Protein
20-abx069720
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  • 1 mg

Human Zyxin (ZYX) Protein
abx069720-1g 1 g
EUR 225

Human Zyxin (ZYX) Protein
abx069720-5g 5 g
EUR 400

Human Zyxin (ZYX) Protein
20-abx069721
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Human Zyxin (ZYX) Protein
abx069721-1g 1 g
EUR 225

Human Zyxin (ZYX) CLIA Kit
20-abx493561
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  • 96 tests
  • 5 × 96 tests
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Human Zyxin(ZYX) ELISA kit
CSB-EL027165HU-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human Zyxin (ZYX) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Zyxin(ZYX) ELISA kit
CSB-EL027165HU-48T 48T
EUR 338.81
Description: Homo sapiens (Human)

Human Zyxin(ZYX) ELISA kit
1-CSB-EL027165HU
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  • 1 plate of 96 wells
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Description: Quantitativesandwich ELISA kit for measuring Human Zyxin(ZYX) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Zyxin(ZYX) ELISA kit
CSB-EL027165HU-96T 96T
EUR 521.25
Description: Homo sapiens (Human)

Human Zyxin (ZYX) ELISA Kit
20-abx153537
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  • 96 tests
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Human Zyxin (ZYX) ELISA Kit
abx555876-96tests 96 tests
EUR 801.6

Human Zyxin (ZYX) ELISA Kit
AE62165HU-48T 48T
EUR 325
Description: Human (Homo sapiens)

Human Zyxin (ZYX) ELISA Kit
AE62165HU-48Tests 48 Tests
EUR 325
Description: Human (Homo sapiens)

Human Zyxin (ZYX) ELISA Kit
AE62165HU-96T 96T
EUR 610
Description: Human (Homo sapiens)

Human Zyxin (ZYX) ELISA Kit
AE62165HU-96Tests 96 Tests
EUR 610
Description: Human (Homo sapiens)

Human Zyxin, ZYX ELISA KIT
ELI-53492h 96 Tests
EUR 988.8

Human Zyxin(ZYX)ELISA Kit
GA-E1505HM-48T 48T
EUR 346.8

Human Zyxin(ZYX)ELISA Kit
GA-E1505HM-48Tests 48 Tests Ask for price

Human Zyxin(ZYX)ELISA Kit
GA-E1505HM-96T 96T
EUR 559.2

Human Zyxin(ZYX)ELISA Kit
GA-E1505HM-96Tests 96 Tests
EUR 388

Human Zyxin (ZYX) ELISA Kit
EKU08257-48T 48T
EUR 555.66

Human Zyxin (ZYX) ELISA Kit
EKU08257-5x96T 5x96T
EUR 3770.55

Human Zyxin (ZYX) ELISA Kit
EKU08257-96T 96T
EUR 793.8

Human Zyxin (ZYX) ELISA Kit
EK11691 96Т
EUR 799

Human Zyxin (ZYX) ELISA Kit
EK20493 96Т
EUR 768

Human Zyxin(ZYX) Elisa Kit
EK714112 96 Wells
EUR 0.42

Human Zyxin (ZYX) ELISA Kit
EKN49368-48T 48T
EUR 345.73

Human Zyxin (ZYX) ELISA Kit
EKN49368-5x96T 5x96T
EUR 2346.03

Human Zyxin (ZYX) ELISA Kit
EKN49368-96T 96T
EUR 493.9

Human Zyxin(ZYX) ELISA kit
EKC36093-48T 48T
EUR 535.99

Human Zyxin(ZYX) ELISA kit
EKC36093-5x96T 5x96T
EUR 3637.08

Human Zyxin(ZYX) ELISA kit
EKC36093-96T 96T
EUR 765.7

Human Zyxin,ZYX ELISA KIT
E6423Hu-1096T 10*96T
EUR 4122

Human Zyxin,ZYX ELISA KIT
E6423Hu-48wells 48 wells
EUR 300

Human Zyxin,ZYX ELISA KIT
E6423Hu-596T 5*96T
EUR 2061

Human Zyxin,ZYX ELISA KIT
E6423Hu-96wells 96 wells
EUR 458

Human Zyxin,ZYX ELISA KIT
JOT-EK6423Hu 96 wells Ask for price
Description: Human

Human Zyxin (ZYX) ELISA Kit
MBS2800280-10x96StripWells 10x96-Strip-Wells
EUR 6255

Human Zyxin (ZYX) ELISA Kit
MBS2800280-48StripWells 48-Strip-Wells
EUR 445

Human Zyxin (ZYX) ELISA Kit
MBS2800280-5x96StripWells 5x96-Strip-Wells
EUR 3290

Human Zyxin (ZYX) ELISA Kit
MBS2800280-96StripWells 96-Strip-Wells
EUR 730

Human Zyxin (ZYX) ELISA Kit
MBS2704301-10x96StripWells 10x96-Strip-Wells
EUR 3130

Human Zyxin (ZYX) ELISA Kit
MBS2704301-24StripWells 24-Strip-Wells
EUR 255

Human Zyxin (ZYX) ELISA Kit
MBS2704301-48StripWells 48-Strip-Wells
EUR 330

Human Zyxin (ZYX) ELISA Kit
MBS2704301-5x96StripWells 5x96-Strip-Wells
EUR 1630

Human Zyxin (ZYX) ELISA Kit
MBS2704301-96StripWells 96-Strip-Wells
EUR 420

Human Zyxin, ZYX ELISA Kit
MBS1607267-10x96StripWells 10x96-Strip-Wells
EUR 3460

Human Zyxin, ZYX ELISA Kit
MBS1607267-48StripWells 48-Strip-Wells
EUR 285

Human Zyxin, ZYX ELISA Kit
MBS1607267-5x96StripWells 5x96-Strip-Wells
EUR 1750

Human Zyxin, ZYX ELISA Kit
MBS1607267-96StripWells 96-Strip-Wells
EUR 425

Human Zyxin (ZYX) ELISA Kit
MBS2019199-10x96StripWells 10x96-Strip-Wells
EUR 5320

Human Zyxin (ZYX) ELISA Kit
MBS2019199-24StripWells 24-Strip-Wells
EUR 360

Human Zyxin (ZYX) ELISA Kit
MBS2019199-48StripWells 48-Strip-Wells
EUR 545

Human Zyxin (ZYX) ELISA Kit
MBS2019199-5x96StripWells 5x96-Strip-Wells
EUR 2915

Human Zyxin (ZYX) ELISA Kit
MBS2019199-96StripWells 96-Strip-Wells
EUR 720

Human Zyxin, ZYX ELISA Kit
MBS923014-10x96StripWells 10x96-Strip-Wells
EUR 5575

Human Zyxin, ZYX ELISA Kit
MBS923014-24StripWellsLIMIT1 24-Strip-Wells(LIMIT1)
EUR 275

Human Zyxin, ZYX ELISA Kit
MBS923014-48StripWells 48-Strip-Wells
EUR 545

Human Zyxin, ZYX ELISA Kit
MBS923014-5x96StripWells 5x96-Strip-Wells
EUR 2920

Human Zyxin, ZYX ELISA Kit
MBS923014-96StripWells 96-Strip-Wells
EUR 765

Human Zyxin (ZYX) ELISA Kit
SEC235Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Zyxin (ZYX) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Zyxin (ZYX) ELISA Kit
SEC235Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Zyxin (ZYX) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Zyxin (ZYX) ELISA Kit
SEC235Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Zyxin (ZYX) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Zyxin (ZYX) ELISA Kit
SEC235Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Zyxin (ZYX) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Zyxin (ZYX) ELISA Kit
4-SEC235Hu
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  • 1 plate of 96 wells
  • 5 plates of 96 wells
  • 10 plates of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Zyxin (ZYX) in samples from tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Zyxin (ZYX) ELISA Kit
RK12444 96T
EUR 322.37

Human Zyxin, ZYX GENLISA ELISA
KBH6423 1 x 96 wells
EUR 286

Recombinant Human Zyxin
MBS7614304-005mg 0.05mg
EUR 345

Recombinant Human Zyxin
MBS7614304-02mg 0.2mg
EUR 635

Recombinant Human Zyxin
MBS7614304-1mg 1mg
EUR 1800

Recombinant Human Zyxin
MBS7614304-5x1mg 5x1mg
EUR 6955

ZYX (untagged)-Human zyxin (ZYX), transcript variant 2
SC319514 10 µg Ask for price

ZYX (untagged)-Human zyxin (ZYX), transcript variant 1
SC117947 10 µg Ask for price

Human Zyxin ELISA Kit (ZYX)
RK02541 96 Tests
EUR 322.37

Zyxin (ZYX) Monoclonal Antibody (Human)
4-MAC235Hu21
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  • 20ul
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  • 1ml
  • 10ml
Description: A Mouse monoclonal antibody against Human Zyxin (ZYX)

Zyxin (ZYX) Polyclonal Antibody (Human)
4-PAC235Hu01
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Description: A Rabbit polyclonal antibody against Human Zyxin (ZYX)

Zyxin (ZYX) Polyclonal Antibody (Human)
4-PAC235Hu02
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Description: A Rabbit polyclonal antibody against Human Zyxin (ZYX)

Human ZYX(Zyxin) ELISA Kit
ELK4891-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ZYX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ZYX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ZYX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ZYX in the samples is then determined by comparing the OD of the samples to the standard curve.

Human ZYX(Zyxin) ELISA Kit
ELK4891-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ZYX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ZYX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ZYX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ZYX in the samples is then determined by comparing the OD of the samples to the standard curve.

Human ZYX/ Zyxin ELISA Kit
E2725Hu 1 Kit
EUR 515
Description: Zyxin-2

Human ZYX/Zyxin ELISA Kit
MBS3803413-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human ZYX/Zyxin ELISA Kit
MBS3803413-48StripWells 48-Strip-Wells
EUR 550

Human ZYX/Zyxin ELISA Kit
MBS3803413-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human ZYX/Zyxin ELISA Kit
MBS3803413-96StripWells 96-Strip-Wells
EUR 765

Human ZYX/Zyxin ELISA Kit
MBS2890380-10x96StripWells 10x96-Strip-Wells
EUR 4000

Human ZYX/Zyxin ELISA Kit
MBS2890380-48StripWells 48-Strip-Wells
EUR 395

Human ZYX/Zyxin ELISA Kit
MBS2890380-5x96StripWells 5x96-Strip-Wells
EUR 2185

Human ZYX/Zyxin ELISA Kit
MBS2890380-96StripWells 96-Strip-Wells
EUR 520

Human ZYX (Zyxin) ELISA Kit
MBS8803889-10x96StripWells 10x96-Strip-Wells
EUR 3130

Human ZYX (Zyxin) ELISA Kit
MBS8803889-48StripWells 48-Strip-Wells
EUR 350

Human ZYX (Zyxin) ELISA Kit
MBS8803889-5x96StripWells 5x96-Strip-Wells
EUR 1710

Human ZYX (Zyxin) ELISA Kit
MBS8803889-96StripWells 96-Strip-Wells
EUR 445

ELISA kit for Human Zyxin (ZYX)
KTE62447-48T 48T
EUR 398.4
Description: Quantitative sandwich ELISA for measuring Human Zyxin (ZYX) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Zyxin (ZYX)
KTE62447-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Human Zyxin (ZYX) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Zyxin (ZYX)
KTE62447-96T 96T
EUR 646.8
Description: Quantitative sandwich ELISA for measuring Human Zyxin (ZYX) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human Zyxin-ZYX-ELISA Kit
QY-E04229 96T
EUR 400

Zyxin (ZYX) Antibody
abx001747-100l 100 µl
EUR 400

Zyxin (ZYX) Antibody
20-abx001747
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Zyxin (ZYX) Antibody
abx001747-20l 20 µl
EUR 175

Zyxin (ZYX) Antibody
abx001747-50l 50 µl
EUR 275

Zyxin (ZYX) Antibody
abx037464-100ug 100 ug
EUR 469.2

Zyxin (ZYX) Antibody
abx037464-1096tests 10 × 96 tests Ask for price

Zyxin (ZYX) Antibody
abx037464-596tests 5 × 96 tests Ask for price

Zyxin (ZYX) Antibody
abx037464-96tests 96 tests
EUR 337.5

Zyxin (ZYX) Antibody
abx116771-100g 100 µg
EUR 612.5

Zyxin (ZYX) Antibody
20-abx116771
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  • 50 ul
  • 150 ul

Zyxin (ZYX) Antibody
abx100030-100l 100 µl
EUR 275

Zyxin (ZYX) Antibody
20-abx100030
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  • 10 ug
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  • 1 mg

Zyxin (ZYX) Antibody
abx100030-1ml 1 ml
EUR 750

Zyxin (ZYX) Antibody
abx100030-200l 200 µl
EUR 337.5

Zyxin (ZYX) Antibody
abx100031-100l 100 µl
EUR 275

Zyxin (ZYX) Antibody
20-abx100031
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  • 10 ug
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  • 1 mg

Zyxin (ZYX) Antibody
abx100031-1ml 1 ml
EUR 750

Zyxin (ZYX) Antibody
abx100031-200l 200 µl
EUR 337.5

Zyxin (ZYX) Antibody
20-abx175170
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  • 20 ug
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  • 100 ug
  • 200 ug
  • 1 mg

Zyxin (ZYX) Antibody
abx175170-1096tests 10 × 96 tests
EUR 787.5

Zyxin (ZYX) Antibody
abx175170-596tests 5 × 96 tests
EUR 350

Zyxin (ZYX) Antibody
abx175170-96tests 96 tests
EUR 275

Zyxin (ZYX) Antibody
abx433465-200g 200 µg
EUR 387.5

Zyxin (ZYX) Antibody
abx433465-200ul 200 ul
EUR 460.8

Zyxin (ZYX) Antibody
abx239764-100l 100 µl Ask for price

Zyxin (ZYX) Antibody
abx239764-100ug 100 ug
EUR 661.2

Zyxin (ZYX) Antibody
abx239764-50l 50 µl
EUR 350

Zyxin (ZYX) Antibody
abx239765-100l 100 µl Ask for price

Zyxin (ZYX) Antibody
abx239765-100ug 100 ug
EUR 610.8

Zyxin (ZYX) Antibody
abx239765-50l 50 µl
EUR 350

ZYX (GFP-tagged) - Human zyxin (ZYX), transcript variant 2
RG200960 10 µg Ask for price

ZYX (GFP-tagged) - Human zyxin (ZYX), transcript variant 1
RG214079 10 µg Ask for price

Methods in molecular cardiology: DHPLC mutation detection analysis.

An increasing number of mutations have been identified in genes involved in cardiac disorders which has led to novel insights in the pathophysiology of inherited cardiac diseases. As a result of these findings, techniques specialised in automated high-throughput analysis are implemented to handle the increasing number of diagnostic genetic requests.

Denaturing high-performance liquid chromatography (DHPLC) is one such novel technique that fulfils the criteria of speed, sensitivity and accuracy. This issue focuses on the basic principle of the technique and illustrates how genetic alterations can be identified.

DHPLC technology for high-throughput detection of mutations in a durum wheat TILLING population

BACKGROUND

Durum wheat (Triticum turgidum L.) is a cereal crop widely grown in the Mediterranean regions; the amber grain is mainly used for the production of pasta, couscous and typical breads. Single nucleotide polymorphism (SNP) detection technologies and high-throughput mutation induction represent a new challenge in wheat breeding to identify allelic variation in large populations.

The TILLING strategy makes use of traditional chemical mutagenesis followed by screening for single base mismatches to identify novel mutant loci. Although TILLING has been combined to several sensitive pre-screening methods for SNP analysis, most rely on expensive equipment. Recently, a new low cost and time saving DHPLC protocol has been used in molecular human diagnostic to detect unknown mutations.

RESULTS

In this work, we developed a new durum wheat TILLING population (cv. Marco Aurelio) using 0.70-0.85% ethyl methane sulfonate (EMS). To investigate the efficiency of the mutagenic treatments, a pilot screening was carried out on 1,140 mutant lines focusing on two target genes (Lycopene epsilon-cyclase, ε-LCY, and Lycopene beta-cyclase, β-LCY) involved in carotenoid metabolism in wheat grains.

We simplify the heteroduplex detection by two low cost methods: the enzymatic cleavage (CelI)/agarose gel technique and the denaturing high-performance liquid chromatography (DHPLC). The CelI/agarose gel approach allowed us to identify 31 mutations, whereas the DHPLC procedure detected a total of 46 mutations for both genes.

All detected mutations were confirmed by direct sequencing. The estimated overall mutation frequency for the pilot assay by the DHPLC methodology resulted to be of 1/77 kb, representing a high probability to detect interesting mutations in the target genes.

CONCLUSIONS

We demonstrated the applicability and efficiency of a new strategy for the detection of induced variability. We produced and characterized a new durum wheat TILLING population useful for a better understanding of key gene functions. The availability of this tool together with TILLING technique will expand the polymorphisms in candidate genes of agronomically important traits in wheat.

Diversity of the microbiota involved in wine and organic apple cider submerged vinegar production as revealed by DHPLC analysis and next-generation sequencing.

Unfiltered vinegar samples collected from three oxidation cycles of the submerged industrial production of each, red wine and organic apple cider vinegars, were sampled in a Slovene vinegar producing company. The samples were systematically collected from the beginning to the end of an oxidation cycle and used for culture-independent microbial analyses carried out by denaturing high pressure liquid chromatography (DHPLC) and Illumina MiSeq sequencing of 16S rRNA gene variable regions.

 

Both approaches showed a very homogeneous bacterial structure during wine vinegar production but more heterogeneous during organic apple cider vinegar production. In all wine vinegar samples Komagataeibacter oboediens (formerly Gluconacetobacter oboediens) was a predominating species. In apple cider vinegar the acetic acid and lactic acid bacteria were two major groups of bacteria.

 

The acetic acid bacterial consortium was composed of Acetobacter and Komagataeibacter with the Komagataeibacter genus outcompeting the Acetobacter in all apple cider vinegar samples at the end of oxidation cycle. Among the lactic acid bacterial consortium two dominating genera were identified, Lactobacillus and Oenococcus, with Oenococcus prevailing with increasing concentration of acetic acid in vinegars. Unexpectedly, a minor genus of the acetic acid bacterial consortium in organic apple cider vinegar was Gluconobacter, suggesting a possible development of the Gluconobacter population with a tolerance against ethanol and acetic acid. Among the accompanying bacteria of the wine vinegar, the genus Rhodococcus was detected, but it decreased substantially by the end of oxidation cycles

Recombinant Human Annexin A2 (ANXA2)
CSB-EP001840HUa0 547 mg Ask for price

Recombinant Human Annexin A2 (ANXA2)
CSB-EP001840HUa0-20ug 20ug Ask for price
Description: 2-339aa

Recombinant Human Annexin A2 (ANXA2)
CSB-EP001840HUb2 548 mg Ask for price

Recombinant Human Annexin A2 (ANXA2)
CSB-EP001840HUb2-20ug 20ug Ask for price
Description: 2-339aa

Recombinant Human Annexin A2 (ANXA2)
CSB-EP001840HUe0 549 mg Ask for price

Recombinant Human Annexin A2 (ANXA2)
CSB-EP001840HUe0-20ug 20ug Ask for price
Description: 2-339aa

Recombinant Human Annexin A2(ANXA2)
AP70034 1mg
EUR 2362

Recombinant Human Annexin A2(ANXA2)
AP71695 1mg
EUR 2362

Recombinant Human Annexin A2(ANXA2)
AP77769 1mg
EUR 2362

Recombinant Human Annexin A2(ANXA2)
AP77903 1mg
EUR 2362

Recombinant Human Annexin A2/ANXA2
AP74937 1mg
EUR 3209

Recombinant Human Annexin A2 (ANXA2)
MBS968326-002mgEColi 0.02mg(E-Coli)
EUR 275

Recombinant Human Annexin A2 (ANXA2)
MBS968326-002mgMammalianCell 0.02mg(Mammalian-Cell)
EUR 425

Recombinant Human Annexin A2 (ANXA2)
MBS968326-002mgYeast 0.02mg(Yeast)
EUR 1000

Recombinant Human Annexin A2 (ANXA2)
MBS968326-01mgEColi 0.1mg(E-Coli)
EUR 460

Recombinant Human Annexin A2 (ANXA2)
MBS968326-01mgMammalianCell 0.1mg(Mammalian-Cell)
EUR 1015

Recombinant Human Annexin A2 (ANXA2)
RPC20900-100ug 100ug
EUR 475.2

Recombinant Human Annexin A2 (ANXA2)
RPC20900-1mg 1mg
EUR 2411.5

Recombinant Human Annexin A2 (ANXA2)
RPC20900-20ug 20ug
EUR 295.7

Recombinant Human Annexin A2 (ANXA2)
RPC23807-100ug 100ug
EUR 1503.7

Recombinant Human Annexin A2 (ANXA2)
RPC23807-20ug 20ug
EUR 484

Recombinant Human Annexin A2 (ANXA2)
RPC25841-100ug 100ug
EUR 585.2

Recombinant Human Annexin A2 (ANXA2)
RPC25841-1mg 1mg
EUR 2706.6

Recombinant Human Annexin A2 (ANXA2)
RPC25841-20ug 20ug
EUR 295.7

Recombinant Human Annexin A2/ANXA2 Protein
MBS2552354-001mg 0.01mg
EUR 180

Recombinant Human Annexin A2/ANXA2 Protein
MBS2552354-005mg 0.05mg
EUR 380

Recombinant Human Annexin A2/ANXA2 Protein
MBS2552354-5x005mg 5x0.05mg
EUR 1690

Recombinant Human Annexin A2/ANXA2 Protein
PKSH032074-10ug 10ug
EUR 129
Description: Human

Recombinant Human Annexin A2/ANXA2 Protein
PKSH032074-50ug 50ug
EUR 375
Description: Human

Recombinant Annexin A2 (ANXA2)
MBS2097690-001mg 0.01mg
EUR 155

Recombinant Annexin A2 (ANXA2)
MBS2097690-005mg 0.05mg
EUR 260

Recombinant Annexin A2 (ANXA2)
MBS2097690-01mg 0.1mg
EUR 375

Recombinant Annexin A2 (ANXA2)
MBS2097690-02mg 0.2mg
EUR 460

Recombinant Annexin A2 (ANXA2)
MBS2097690-05mg 0.5mg
EUR 860

Recombinant Annexin A2 (ANXA2)
MBS2030860-001mg 0.01mg
EUR 170

Recombinant Annexin A2 (ANXA2)
MBS2030860-005mg 0.05mg
EUR 295

Recombinant Annexin A2 (ANXA2)
MBS2030860-01mg 0.1mg
EUR 445

Recombinant Annexin A2 (ANXA2)
MBS2030860-02mg 0.2mg
EUR 540

Recombinant Annexin A2 (ANXA2)
MBS2030860-05mg 0.5mg
EUR 1025

Recombinant Annexin A2 (ANXA2)
MBS2009095-001mg 0.01mg
EUR 160

Recombinant Annexin A2 (ANXA2)
MBS2009095-005mg 0.05mg
EUR 280

Recombinant Annexin A2 (ANXA2)
MBS2009095-01mg 0.1mg
EUR 405

Recombinant Annexin A2 (ANXA2)
MBS2009095-02mg 0.2mg
EUR 490

Recombinant Annexin A2 (ANXA2)
MBS2009095-05mg 0.5mg
EUR 930

Recombinant Annexin A2 (ANXA2)
RPU55312-100ug 100ug
EUR 431.2

Recombinant Annexin A2 (ANXA2)
RPU55312-1mg 1mg
EUR 1911

Recombinant Annexin A2 (ANXA2)
RPU55312-50ug 50ug
EUR 346.5

Recombinant Annexin A2 (ANXA2)
RPU57039-100ug 100ug
EUR 470.4

Recombinant Annexin A2 (ANXA2)
RPU57039-1mg 1mg
EUR 2184

Recombinant Annexin A2 (ANXA2)
RPU57039-50ug 50ug
EUR 385

Recombinant Annexin A2 (ANXA2)
RPU52100-100ug 100ug
EUR 517

Recombinant Annexin A2 (ANXA2)
RPU52100-1mg 1mg
EUR 2293.2

Recombinant Annexin A2 (ANXA2)
RPU52100-50ug 50ug
EUR 415.8

Recombinant Annexin A2 (ANXA2)
RPU40298-100ug 100ug
EUR 468.6

Recombinant Annexin A2 (ANXA2)
RPU40298-1mg 1mg
EUR 2074.8

Recombinant Annexin A2 (ANXA2)
RPU40298-50ug 50ug
EUR 376.2

Recombinant Annexin A2 (ANXA2)
RPB944Hu01 10ug
EUR 152

Recombinant Annexin A2 (ANXA2)
4-RPB944Hu01
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  • 5 mg
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  • 500 ug
  • 1 mg
Description: Recombinant Human Annexin A2 expressed in: E.coli

Recombinant Annexin A2 (ANXA2)
RPB944Hu03 10ug
EUR 154

Recombinant Annexin A2 (ANXA2)
RPB944Hu04 10ug
EUR 154

Recombinant Annexin A2 (ANXA2)
RPB944Mu01 10ug
EUR 168

Recombinant Annexin A2 (ANXA2)
4-RPB944Mu01
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  • 1 mg
Description: Recombinant Mouse Annexin A2 expressed in: E.coli

Recombinant Annexin A2 (ANXA2)
RPB944Mu02 10ug
EUR 140

Recombinant Annexin A2 (ANXA2)
RPB944Ra01 10ug
EUR 140

Recombinant Annexin A2 (ANXA2)
4-RPB944Ra01
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Description: Recombinant Rat Annexin A2 expressed in: E.coli

Annexin A2 (ANXA2) (NM_001002858) Human Recombinant Protein
E45H36004M5 20 ug
EUR 785.42

Annexin A2 (ANXA2) (NM_001002857) Human Recombinant Protein
E45H39512M5 20 ug
EUR 785.42

Annexin A2 (ANXA2) (NM_004039) Human Recombinant Protein
E45H39558M5 20 ug
EUR 785.42

Annexin A2 (ANXA2) (NM_001002857) Human Recombinant Protein
E45H32432E0 50 ug
EUR 528.13

Annexin A2 (ANXA2) (NM_001136015) Human Recombinant Protein
E45H33307M5 20 ug
EUR 785.42

Annexin A2 (ANXA2) (NM_001136015) Human Recombinant Protein
PROTP07355 Regular: 20ug
EUR 1249
Description: Recombinant protein of human annexin A2 (ANXA2), transcript variant 4

Human ANXA2 (Annexin A2) Recombinant Protein
MBS698047-005mg 0.05mg
EUR 580

Human ANXA2 (Annexin A2) Recombinant Protein
MBS698047-02mg 0.2mg
EUR 1290

Human ANXA2 (Annexin A2) Recombinant Protein
MBS698047-05mg 0.5mg
EUR 2350

Human ANXA2 (Annexin A2) Recombinant Protein
MBS698047-1mg 1mg
EUR 3585

Recombinant Dog Annexin A2 (ANXA2)
MBS1482145-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1215

Recombinant Dog Annexin A2 (ANXA2)
MBS1482145-002mgEColi 0.02mg(E-Coli)
EUR 870

Recombinant Dog Annexin A2 (ANXA2)
MBS1482145-002mgYeast 0.02mg(Yeast)
EUR 1005

Recombinant Dog Annexin A2 (ANXA2)
MBS1482145-01mgEColi 0.1mg(E-Coli)
EUR 1045

Recombinant Dog Annexin A2 (ANXA2)
MBS1482145-01mgYeast 0.1mg(Yeast)
EUR 1145

Recombinant Pig Annexin A2 (ANXA2)
MBS958396-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1215

Recombinant Pig Annexin A2 (ANXA2)
MBS958396-002mgEColi 0.02mg(E-Coli)
EUR 870

Recombinant Pig Annexin A2 (ANXA2)
MBS958396-002mgYeast 0.02mg(Yeast)
EUR 1005

Recombinant Pig Annexin A2 (ANXA2)
MBS958396-01mgEColi 0.1mg(E-Coli)
EUR 1045

Recombinant Pig Annexin A2 (ANXA2)
MBS958396-01mgYeast 0.1mg(Yeast)
EUR 1145

Recombinant Human Annexin A2/ANXA2 Protein, No Tag
E40KMH669 20ug
EUR 495

Recombinant Mouse Annexin A2 (Anxa2)
CSB-YP001840MO 10552 mg Ask for price

Recombinant Mouse Annexin A2 (Anxa2)
CSB-YP001840MO-100ug 100ug Ask for price
Description: 2-339aa

Recombinant Mouse Annexin A2 (Anxa2)
CSB-YP001840MO-1mg 1mg Ask for price
Description: 2-339aa

Recombinant Mouse Annexin A2 (Anxa2)
CSB-YP001840MO-20ug 20ug Ask for price
Description: 2-339aa

Recombinant Mouse Annexin A2 (Anxa2)
CSB-MP001840MO 10172 mg Ask for price

Recombinant Mouse Annexin A2 (Anxa2)
CSB-MP001840MO-100ug 100ug Ask for price
Description: 2-339aa

Recombinant Mouse Annexin A2 (Anxa2)
CSB-MP001840MO-1mg 1mg Ask for price
Description: 2-339aa

Recombinant Mouse Annexin A2 (Anxa2)
CSB-MP001840MO-20ug 20ug Ask for price
Description: 2-339aa

Recombinant Mouse Annexin A2 (Anxa2)
CSB-EP001840MO 10290 mg Ask for price

Recombinant Mouse Annexin A2 (Anxa2)
CSB-EP001840MO-100ug 100ug Ask for price
Description: 2-339aa

Recombinant Mouse Annexin A2 (Anxa2)
CSB-EP001840MO-1mg 1mg Ask for price
Description: 2-339aa

Recombinant Mouse Annexin A2 (Anxa2)
CSB-EP001840MO-20ug 20ug Ask for price
Description: 2-339aa

Recombinant Sheep Annexin A2 (ANXA2)
MBS1147005-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1215

Recombinant Sheep Annexin A2 (ANXA2)
MBS1147005-002mgEColi 0.02mg(E-Coli)
EUR 870

Recombinant Sheep Annexin A2 (ANXA2)
MBS1147005-002mgYeast 0.02mg(Yeast)
EUR 1005

Recombinant Sheep Annexin A2 (ANXA2)
MBS1147005-01mgEColi 0.1mg(E-Coli)
EUR 1045

Recombinant Sheep Annexin A2 (ANXA2)
MBS1147005-01mgYeast 0.1mg(Yeast)
EUR 1145

Recombinant Mouse Annexin A2 (Anxa2)
MBS950991-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1210

Recombinant Mouse Annexin A2 (Anxa2)
MBS950991-002mgEColi 0.02mg(E-Coli)
EUR 865

Recombinant Mouse Annexin A2 (Anxa2)
MBS950991-002mgYeast 0.02mg(Yeast)
EUR 1000

Recombinant Mouse Annexin A2 (Anxa2)
MBS950991-01mgEColi 0.1mg(E-Coli)
EUR 1040

Recombinant Mouse Annexin A2 (Anxa2)
MBS950991-01mgYeast 0.1mg(Yeast)
EUR 1135

Recombinant Bovine Annexin A2 (ANXA2)
MBS954139-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1215

Recombinant Bovine Annexin A2 (ANXA2)
MBS954139-002mgEColi 0.02mg(E-Coli)
EUR 870

Recombinant Bovine Annexin A2 (ANXA2)
MBS954139-002mgYeast 0.02mg(Yeast)
EUR 1005

Recombinant Bovine Annexin A2 (ANXA2)
MBS954139-01mgEColi 0.1mg(E-Coli)
EUR 1045

Recombinant Bovine Annexin A2 (ANXA2)
MBS954139-01mgYeast 0.1mg(Yeast)
EUR 1145

Recombinant Chicken Annexin A2 (ANXA2)
MBS951249-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1215

Recombinant Chicken Annexin A2 (ANXA2)
MBS951249-002mgEColi 0.02mg(E-Coli)
EUR 870

Recombinant Chicken Annexin A2 (ANXA2)
MBS951249-002mgYeast 0.02mg(Yeast)
EUR 1005

Recombinant Chicken Annexin A2 (ANXA2)
MBS951249-01mgEColi 0.1mg(E-Coli)
EUR 1045

Recombinant Chicken Annexin A2 (ANXA2)
MBS951249-01mgYeast 0.1mg(Yeast)
EUR 1145

Recombinant Pongo abelii Annexin A2 (ANXA2)
MBS1308108-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1215

Recombinant Pongo abelii Annexin A2 (ANXA2)
MBS1308108-002mgEColi 0.02mg(E-Coli)
EUR 870

Recombinant Pongo abelii Annexin A2 (ANXA2)
MBS1308108-002mgYeast 0.02mg(Yeast)
EUR 1005

Recombinant Pongo abelii Annexin A2 (ANXA2)
MBS1308108-01mgEColi 0.1mg(E-Coli)
EUR 1045

Recombinant Pongo abelii Annexin A2 (ANXA2)
MBS1308108-01mgYeast 0.1mg(Yeast)
EUR 1145

Human Annexin A2 (ANXA2)
1-CSB-MP001840HU
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Description: Recombinant Human Annexin A2(ANXA2) expressed in Mammalian cell

Human Annexin A2 (ANXA2)
1-CSB-EP001840HU
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Description: Recombinant Human Annexin A2(ANXA2) expressed in E.coli

Human Annexin A2 (ANXA2)
1-CSB-EP001840HUa0
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Description: Recombinant Human Annexin A2(ANXA2) expressed in E.coli

Human Annexin A2 (ANXA2)
1-CSB-EP001840HUb2
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Description: Recombinant Human Annexin A2(ANXA2) expressed in E.coli

Human Annexin A2 (ANXA2)
1-CSB-EP001840HUe0
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  • 1MG
Description: Recombinant Human Annexin A2(ANXA2) expressed in E.coli

Recombinant Cervus elaphus Annexin A2 (ANXA2)
MBS1334880-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1215

Recombinant Cervus elaphus Annexin A2 (ANXA2)
MBS1334880-002mgEColi 0.02mg(E-Coli)
EUR 870

Recombinant Cervus elaphus Annexin A2 (ANXA2)
MBS1334880-002mgYeast 0.02mg(Yeast)
EUR 1005

Recombinant Cervus elaphus Annexin A2 (ANXA2)
MBS1334880-01mgEColi 0.1mg(E-Coli)
EUR 1045

Recombinant Cervus elaphus Annexin A2 (ANXA2)
MBS1334880-01mgYeast 0.1mg(Yeast)
EUR 1145

Human Annexin A2 (ANXA2) Protein
abx065365-100g 100 µg
EUR 512.5

Human Annexin A2 (ANXA2) Protein
20-abx065365
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  • 1 mg

Human Annexin A2 (ANXA2) Protein
abx065365-10g 10 µg
EUR 212.5

Human Annexin A2 (ANXA2) Protein
abx065365-50g 50 µg
EUR 362.5

Human Annexin A2 (ANXA2) Protein
abx260570-10g 10 µg
EUR 325

Human Annexin A2 (ANXA2) Protein
abx260570-2g 2 µg
EUR 225

Human Annexin A2 (ANXA2) Protein
abx263373-1mg 1 mg
EUR 225

Human Annexin A2 (ANXA2) Protein
abx263373-25mg 2.5 mg
EUR 325

Annexin A2/ANXA2
E21-205 10ug
EUR 225
Description: Recombinant Human Annexin A2 is produced by our E.coli expression system and the target gene encoding Ser2-Asp339 is expressed.

Annexin A2/ANXA2
MBS8532871-001mg 0.01mg
EUR 305

Annexin A2/ANXA2
MBS8532871-5x001mg 5x0.01mg
EUR 1220

Human Annexin A2 (ANXA2) CLIA Kit
20-abx190397
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  • 96 tests
  • 5 × 96 tests
  • 10 × 96 tests

Human Annexin A2 (ANXA2) CLIA Kit
abx195131-96tests 96 tests
EUR 618.75

Human Annexin A2 (ANXA2) CLIA Kit
EKU10027-48T 48T
EUR 666.82

Human Annexin A2 (ANXA2) CLIA Kit
EKU10027-5x96T 5x96T
EUR 4524.85

Human Annexin A2 (ANXA2) CLIA Kit
EKU10027-96T 96T
EUR 952.6

Human Annexin A2 (ANXA2) CLIA Kit
EKN51138-48T 48T
EUR 414.89

Human Annexin A2 (ANXA2) CLIA Kit
EKN51138-5x96T 5x96T
EUR 2815.33

Human Annexin A2 (ANXA2) CLIA Kit
EKN51138-96T 96T
EUR 592.7

Human Annexin A2 (ANXA2) CLIA Kit
MBS2709004-10x96StripWells 10x96-Strip-Wells
EUR 3735

Human Annexin A2 (ANXA2) CLIA Kit
MBS2709004-24StripWells 24-Strip-Wells
EUR 275

Human Annexin A2 (ANXA2) CLIA Kit
MBS2709004-48StripWells 48-Strip-Wells
EUR 365

Human Annexin A2 (ANXA2) CLIA Kit
MBS2709004-5x96StripWells 5x96-Strip-Wells
EUR 1935

Human Annexin A2 (ANXA2) CLIA Kit
MBS2709004-96StripWells 96-Strip-Wells
EUR 480

Human Annexin A2 (ANXA2) CLIA Kit
MBS2000892-10x96StripWells 10x96-Strip-Wells
EUR 6205

Human Annexin A2 (ANXA2) CLIA Kit
MBS2000892-24StripWells 24-Strip-Wells
EUR 405

Human Annexin A2 (ANXA2) CLIA Kit
MBS2000892-48StripWells 48-Strip-Wells
EUR 625

Human Annexin A2 (ANXA2) CLIA Kit
MBS2000892-5x96StripWells 5x96-Strip-Wells
EUR 3400

Human Annexin A2 (ANXA2) CLIA Kit
MBS2000892-96StripWells 96-Strip-Wells
EUR 845

Human Annexin A2 (ANXA2)CLIA Kit
SCB944Hu-10x96wellstestplate 10x96-wells test plate
EUR 6777.36
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Annexin A2 (ANXA2) in serum, plasma and other biological fluids.

Human Annexin A2 (ANXA2)CLIA Kit
SCB944Hu-1x48wellstestplate 1x48-wells test plate
EUR 663.31
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Annexin A2 (ANXA2) in serum, plasma and other biological fluids.

Human Annexin A2 (ANXA2)CLIA Kit
SCB944Hu-1x96wellstestplate 1x96-wells test plate
EUR 896.16
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Annexin A2 (ANXA2) in serum, plasma and other biological fluids.

Human Annexin A2 (ANXA2)CLIA Kit
SCB944Hu-5x96wellstestplate 5x96-wells test plate
EUR 3672.72
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Annexin A2 (ANXA2) in serum, plasma and other biological fluids.

Human Annexin A2 (ANXA2) CLIA Kit
4-SCB944Hu
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  • 1 plate of 96 wells
  • 5 plates of 96 wells
  • 10 plates of 96 wells
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Annexin A2 (ANXA2)Serum, plasma and other biological fluids

ANXA2 Annexin A2 Human protein
PROTP07355-1 Regular: 5ug
EUR 380.4
Description: The Human Annexin A2 produced from Human Adipose Tissue has a molecular mass of 38.472kDa (calculated without glycosylation) containing 338 amino acid residues.

Human Annexin A2,Anxa2 ELISA Kit
201-12-1089 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Annexin A2 (ANXA2) ELISA Kit
20-abx150668
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  • 96 tests
  • 5 × 96 tests
  • 10 × 96 tests

Human Annexin A2 (ANXA2) ELISA Kit
abx573296-96tests 96 tests
EUR 801.6

Human Annexin A2 (ANXA2) ELISA Kit
DLR-ANXA2-Hu 96T
EUR 443
Description: serum, plasma, tissue homogenates or other biological fluids.

Human Annexin A2 (ANXA2) ELISA Kit
DLR-ANXA2-Hu-48T 48T
EUR 597.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Annexin A2 (ANXA2) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Annexin A2 (ANXA2) ELISA Kit
DLR-ANXA2-Hu-96T 96T
EUR 776.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Annexin A2 (ANXA2) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Annexin A2 (ANXA2) ELISA Kit
abx251337-96tests 96 tests
EUR 556.25
plasmid 3 plasmid bp plasmid cloning plasmid def plasmid dna plasmid dna isolation plasmid gene plasmid gene expression plasmid hr plasmid map plasmid purification plasmid sequence plasmid sequence analysis plasmid structure plasmid transformation plasmid vector plasmidal plasmidid plasmidin plasmido plasmids bioshock plasmids define plasmids definition plasmids function

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