Efficient IDUA Gene Mutation Detection with Combined Use of dHPLC and Dried Blood

Using COLD-PCR, DHPLC and GeneScanning for the extremely delicate detection of c-KIT somatic mutations in canine mast cell tumours. 

The traditional polymerase chain response (PCR)/sequencing strategies could also be poorly suited to the detection of somatic mutations in canine mast cell tumour (MCT) samples owing to restricted sensitivity.

This research was geared toward establishing novel and extra delicate strategies, assessing their restrict of detection and evaluating their sensitivity with standard strategies.Two completely different ‘driver’ somatic mutations of c-KIT, along with the wild-type counterparts, have been cloned in plasmids to organize customary samples with identified concentrations of mutated alleles in a background of wild-type alleles; the plasmids requirements have been assayed utilizing both standard or novel, extremely delicate method.

Standard PCR/sequencing confirmed a sensitivity of 50-20%. Conversely, all of the novel strategies obtained greater sensitivities allowed reaching as little as 2.5-1.2% of the mutated DNA.The research demonstrates that early standard strategies may possible have underestimated the prevalence of KIT mutations of MCTs, subsequently affecting the evaluation of their relevance in prognosis and tyrosine kinase inhibitor (TKI) remedy effectiveness.

Mouse Filaggrin (FLG) ELISA Kit
DLR-FLG-Mu-48T	DL Develop	48T	EUR 527
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Filaggrin (FLG) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit
DLR-FLG-Mu-96T	DL Develop	96T	EUR 688
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Filaggrin (FLG) in samples from tissue homogenates, cell lysates or other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit
RDR-FLG-Mu-48Tests	Reddot Biotech	48 Tests	EUR 557

Mouse Filaggrin (FLG) ELISA Kit
RDR-FLG-Mu-96Tests	Reddot Biotech	96 Tests	EUR 774

Recombinant Filaggrin (FLG)
4-RPJ103Hu01	Cloud-Clone	EUR 816.80 EUR 322.00 EUR 2788.00 EUR 996.00 EUR 1892.00 EUR 610.00 EUR 6820.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Filaggrin expressed in: E.coli

Recombinant Filaggrin (FLG)
4-RPJ103Mu01	Cloud-Clone	EUR 530.08 EUR 245.00 EUR 1712.80 EUR 637.60 EUR 1175.20 EUR 418.00 EUR 4132.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Mouse Filaggrin expressed in: E.coli

Human Filaggrin (FLG)
1-CSB-YP008712HU	Cusabio	EUR 430.00 EUR 234.00 EUR 1508.00 EUR 642.00 EUR 1009.00 EUR 291.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Filaggrin(FLG),partial expressed in Yeast

Human Filaggrin (FLG)
1-CSB-BP008712HU	Cusabio	EUR 840.00 EUR 332.00 EUR 2170.00 EUR 1135.00 EUR 1579.00 EUR 470.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Filaggrin(FLG) ,partial expressed in Baculovirus

Human Filaggrin (FLG)
1-CSB-EP008712HU	Cusabio	EUR 380.00 EUR 214.00 EUR 1309.00 EUR 560.00 EUR 873.00 EUR 262.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Filaggrin(FLG),partial expressed in E.coli

Filaggrin (FLG) Antibody
20-abx109789	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Filaggrin (FLG) Antibody
20-abx339302	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

Filaggrin (FLG) Antibody
20-abx339766	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

Filaggrin (FLG) Antibody
20-abx100249	Abbexa	EUR 425.00 EUR 133.00 EUR 1205.00 EUR 578.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Filaggrin (FLG) Antibody
20-abx100250	Abbexa	EUR 439.00 EUR 133.00 EUR 1233.00 EUR 592.00 EUR 328.00	100 ug 10 ug 1 mg 200 ug 50 ug

Filaggrin (FLG) Antibody
20-abx133148	Abbexa	EUR 300.00 EUR 439.00 EUR 189.00	100 ul 200 ul 30 ul

Filaggrin (FLG) Antibody
20-abx176473	Abbexa	EUR 1233.00 EUR 592.00	1 mg 200 ug

Filaggrin (FLG) Antibody
20-abx172440	Abbexa	EUR 857.00 EUR 439.00	1 mg 200 ug

Human Filaggrin (FLG) Protein
20-abx066657	Abbexa	EUR 1121.00 EUR 411.00 EUR 3724.00 EUR 1358.00 EUR 759.00	100 ug 10 ug 1 mg 200 ug 50 ug

Anti-Filaggrin/FLG Antibody
PB9870	BosterBio	100ug/vial	EUR 334

Filaggrin (FLG) Antibody (FITC)
20-abx106841	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Filaggrin (FLG) Antibody (HRP)
20-abx108261	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Filaggrin (FLG) Antibody (Biotin)
20-abx105422	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

Mouse Filaggrin (FLG) Protein
20-abx066658	Abbexa	EUR 732.00 EUR 286.00 EUR 2305.00 EUR 885.00 EUR 523.00	100 ug 10 ug 1 mg 200 ug 50 ug

Filaggrin (FLG) Antibody (FITC)
20-abx271155	Abbexa	EUR 495.00 EUR 258.00 EUR 1455.00 EUR 676.00 EUR 398.00	100 ug 10 ug 1 mg 200 ug 50 ug

Filaggrin (FLG) Antibody (Biotin)
20-abx271421	Abbexa	EUR 467.00 EUR 244.00 EUR 1344.00 EUR 634.00 EUR 342.00	100 ug 10 ug 1 mg 200 ug 50 ug

Filaggrin (FLG) Antibody (Biotin)
20-abx272419	Abbexa	EUR 453.00 EUR 244.00 EUR 1316.00 EUR 620.00 EUR 342.00	100 ug 10 ug 1 mg 200 ug 50 ug

Filaggrin (FLG) Antibody (FITC)
20-abx273568	Abbexa	EUR 481.00 EUR 244.00 EUR 1414.00 EUR 662.00 EUR 356.00	100 ug 10 ug 1 mg 200 ug 50 ug

Human Filaggrin (FLG)CLIA Kit
SCJ103Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5647.8
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Filaggrin (FLG) in Tissue homogenates and other biological fluids.

Human Filaggrin (FLG)CLIA Kit
SCJ103Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 552.76
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Filaggrin (FLG) in Tissue homogenates and other biological fluids.

Human Filaggrin (FLG)CLIA Kit
SCJ103Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 746.8
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Filaggrin (FLG) in Tissue homogenates and other biological fluids.

Human Filaggrin (FLG)CLIA Kit
SCJ103Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3060.6
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Filaggrin (FLG) in Tissue homogenates and other biological fluids.

Human Filaggrin (FLG) CLIA Kit
4-SCJ103Hu	Cloud-Clone	EUR 5698.00 EUR 3061.00 EUR 747.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Filaggrin (FLG)Tissue homogenates and other biological fluids

Filaggrin (FLG) Polyclonal Antibody (Human)
4-PAJ103Hu01	Cloud-Clone	EUR 247.00 EUR 2510.00 EUR 625.00 EUR 310.00 EUR 214.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG)

Human Filaggrin (FLG) ELISA Kit
SEJ103Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 4731.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Filaggrin (FLG) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Filaggrin (FLG) ELISA Kit
SEJ103Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 477.3
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Filaggrin (FLG) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Filaggrin (FLG) ELISA Kit
SEJ103Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 639
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Filaggrin (FLG) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Filaggrin (FLG) ELISA Kit
SEJ103Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2575.5
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Filaggrin (FLG) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Filaggrin (FLG) ELISA Kit
4-SEJ103Hu	Cloud-Clone	EUR 4782.00 EUR 2526.00 EUR 640.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Filaggrin (FLG) in samples from tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Filaggrin(FLG) ELISA kit
CSB-EL008712HU-24T	Cusabio	1 plate of 24 wells	EUR 165
Description: Quantitativesandwich ELISA kit for measuring Human Filaggrin (FLG) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Filaggrin(FLG) ELISA kit
1-CSB-EL008712HU	Cusabio	EUR 804.00 EUR 5099.00 EUR 2704.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Filaggrin(FLG) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human FLG/ Filaggrin ELISA Kit
E0920Hu	Sunlong	1 Kit	EUR 571

Human Filaggrin (FLG)ELISA Kit
201-12-2700	SunredBio	96 tests	EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Filaggrin (FLG) CLIA Kit
20-abx190272	Abbexa	EUR 7911.00 EUR 4215.00 EUR 973.00	10 × 96 tests 5 × 96 tests 96 tests

Human Filaggrin (FLG) ELISA Kit
abx572526-96tests	Abbexa	96 tests	EUR 668

Human Filaggrin (FLG) ELISA Kit
20-abx151555	Abbexa	EUR 7378.00 EUR 3933.00 EUR 911.00	10 × 96 tests 5 × 96 tests 96 tests

Human Filaggrin ELISA Kit (FLG)
RK01403	Abclonal	96 Tests	EUR 521

Human Filaggrin(FLG)ELISA Kit
QY-E01412	Qayee Biotechnology	96T	EUR 374

Human FLG(Filaggrin) ELISA Kit
EH1353	FN Test	96T	EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Human Filaggrin, FLG ELISA KIT
ELI-03920h	Lifescience Market	96 Tests	EUR 824

Filaggrin (FLG) Polyclonal Antibody (Human), APC
4-PAJ103Hu01-APC	Cloud-Clone	EUR 345.00 EUR 3275.00 EUR 912.00 EUR 440.00 EUR 219.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with APC.

Filaggrin (FLG) Polyclonal Antibody (Human), Biotinylated
4-PAJ103Hu01-Biotin	Cloud-Clone	EUR 311.00 EUR 2460.00 EUR 727.00 EUR 381.00 EUR 219.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with Biotin.

Filaggrin (FLG) Polyclonal Antibody (Human), Cy3
4-PAJ103Hu01-Cy3	Cloud-Clone	EUR 419.00 EUR 4325.00 EUR 1175.00 EUR 545.00 EUR 251.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with Cy3.

Filaggrin (FLG) Polyclonal Antibody (Human), FITC
4-PAJ103Hu01-FITC	Cloud-Clone	EUR 296.00 EUR 2640.00 EUR 750.00 EUR 372.00 EUR 195.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with FITC.

Filaggrin (FLG) Polyclonal Antibody (Human), HRP
4-PAJ103Hu01-HRP	Cloud-Clone	EUR 316.00 EUR 2855.00 EUR 807.00 EUR 398.00 EUR 206.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with HRP.

Filaggrin (FLG) Polyclonal Antibody (Human), PE
4-PAJ103Hu01-PE	Cloud-Clone	EUR 296.00 EUR 2640.00 EUR 750.00 EUR 372.00 EUR 195.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with PE.

ELISA kit for Human FLG (Filaggrin)
ELK3513	ELK Biotech	1 plate of 96 wells	EUR 432
Description: A sandwich ELISA kit for detection of Filaggrin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit
SEJ103Mu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 4862.4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Filaggrin (FLG) in tissue homogenates, cell lysates and other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit
SEJ103Mu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 488.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Filaggrin (FLG) in tissue homogenates, cell lysates and other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit
SEJ103Mu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 654.4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Filaggrin (FLG) in tissue homogenates, cell lysates and other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit
SEJ103Mu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2644.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Filaggrin (FLG) in tissue homogenates, cell lysates and other biological fluids.

Mouse Filaggrin (FLG) ELISA Kit
4-SEJ103Mu	Cloud-Clone	EUR 4913.00 EUR 2595.00 EUR 655.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Filaggrin (FLG) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Filaggrin(FLG) ELISA kit
CSB-EL008712MO-24T	Cusabio	1 plate of 24 wells	EUR 165
Description: Quantitativesandwich ELISA kit for measuring Mouse Filaggrin (FLG) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Filaggrin(FLG) ELISA kit
1-CSB-EL008712MO	Cusabio	EUR 804.00 EUR 5099.00 EUR 2704.00	1 plate of 96 wells 10 plates of 96 wells each 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Mouse Filaggrin(FLG) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Filaggrin ELISA Kit (FLG)
RK02813	Abclonal	96 Tests	EUR 521

Mouse Flg/ Filaggrin ELISA Kit
E0543Mo	Sunlong	1 Kit	EUR 571

Mouse Filaggrin (FLG) ELISA Kit
abx573034-96tests	Abbexa	96 tests	EUR 668

Mouse Filaggrin (FLG) CLIA Kit
20-abx495617	Abbexa	EUR 7973.00 EUR 4246.00 EUR 981.00	10 × 96 tests 5 × 96 tests 96 tests

Mouse Filaggrin (FLG) ELISA Kit
20-abx154027	Abbexa	EUR 7378.00 EUR 3933.00 EUR 911.00	10 × 96 tests 5 × 96 tests 96 tests

Mouse Filaggrin (FLG) ELISA Kit
abx257768-96tests	Abbexa	96 tests	EUR 746

Mouse Filaggrin(FLG)ELISA Kit
QY-E21727	Qayee Biotechnology	96T	EUR 374

Mouse FLG(Filaggrin) ELISA Kit
EM1853	FN Test	96T	EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 9.375pg/ml

Mouse Filaggrin, Flg ELISA KIT
ELI-03919m	Lifescience Market	96 Tests	EUR 865

Filaggrin (FLG) Polyclonal Antibody (Human), APC-Cy7
4-PAJ103Hu01-APC-Cy7	Cloud-Clone	EUR 571.00 EUR 6430.00 EUR 1705.00 EUR 760.00 EUR 319.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with APC-Cy7.

Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat)
4-PAJ103Mu01	Cloud-Clone	EUR 251.00 EUR 2576.00 EUR 640.00 EUR 316.00 EUR 215.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG)

ELISA kit for Mouse FLG (Filaggrin)
ELK6992	ELK Biotech	1 plate of 96 wells	EUR 432
Description: A sandwich ELISA kit for detection of Filaggrin from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Human FLG-Ab (Anti-Filaggrin)
E-EL-H0283	Elabscience Biotech	1 plate of 96 wells	EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human FLG-Ab (Anti-Filaggrin) in samples from Serum, Plasma, Cell supernatant

Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), APC
4-PAJ103Mu01-APC	Cloud-Clone	EUR 351.00 EUR 3365.00 EUR 935.00 EUR 449.00 EUR 222.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with APC.

Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), Biotinylated
4-PAJ103Mu01-Biotin	Cloud-Clone	EUR 316.00 EUR 2526.00 EUR 744.00 EUR 387.00 EUR 221.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with Biotin.

Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), Cy3
4-PAJ103Mu01-Cy3	Cloud-Clone	EUR 427.00 EUR 4445.00 EUR 1205.00 EUR 557.00 EUR 254.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with Cy3.

Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), FITC
4-PAJ103Mu01-FITC	Cloud-Clone	EUR 301.00 EUR 2712.00 EUR 768.00 EUR 379.00 EUR 197.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with FITC.

Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), HRP
4-PAJ103Mu01-HRP	Cloud-Clone	EUR 321.00 EUR 2933.00 EUR 827.00 EUR 405.00 EUR 209.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with HRP.

Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), PE
4-PAJ103Mu01-PE	Cloud-Clone	EUR 301.00 EUR 2712.00 EUR 768.00 EUR 379.00 EUR 197.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with PE.

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561) Antibody
BNC041561-100	Biotium	100uL	EUR 199
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561),CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561) Antibody
BNC041561-500	Biotium	500uL	EUR 544
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561),CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562) Antibody
BNC041562-100	Biotium	100uL	EUR 199
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562),CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562) Antibody
BNC041562-500	Biotium	500uL	EUR 544
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562),CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563) Antibody
BNC041563-100	Biotium	100uL	EUR 233
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563), CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563) Antibody
BNC041563-500	Biotium	500uL	EUR 545
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563), CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945) Antibody
BNC041945-100	Biotium	100uL	EUR 233
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945), CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945) Antibody
BNC041945-500	Biotium	500uL	EUR 545
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945), CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R) Antibody
BNC041957-100	Biotium	100uL	EUR 233
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R), CF405S conjugate, Concentration: 0.1mg/mL

Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R) Antibody
BNC041957-500	Biotium	500uL	EUR 545
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R), CF405S conjugate, Concentration: 0.1mg/mL

Environment friendly IDUA Gene Mutation Detection with Mixed Use of dHPLC and Dried Blood Samples

Aims. Growth of a easy mutation directed technique with a view to enable reducing the price of mutation testing utilizing an simply obtainable organic materials. Evaluation of the feasibility of such technique was examined utilizing a GC-rich amplicon. Design and Strategies. A way of denaturing high-performance liquid chromatography (dHPLC) was improved and applied as a method for the detection of variants in exon 9 of the IDUA gene. The optimized technique was examined in 500 genomic DNA samples obtained from dried blood spots (DBS).

Outcomes. With this dHPLC method it was doable to detect completely different variants, together with the widespread p.Trp402Ter mutation within the IDUA gene. The excessive GC content material didn’t intervene with the decision and reliability of this system, and discrimination of G-C transversions was additionally achieved. Conclusion. This PCR-based dHPLC technique is proved to be a fast, a delicate, and a very good possibility for screening quite a few samples obtained from DBS. Moreover, it resulted within the constant detection of clearly distinguishable profiles of the widespread p.Trp402Ter IDUA mutation with an advantageous stability of value and technical necessities.

Use of PCR-DHPLC with fluorescence detection for the characterization of the bacterial variety throughout cassava (Manihot esculenta Crantz) fermentation 

Denaturing high-performance liquid chromatography (DHPLC) has been described as an appropriate technique to check DNA polymorphisms. Right here, cassava (Manihot esculenta Crantz) fermentation liquor was examined utilizing DHPLC evaluation to characterize the bacterial variety through the fermentation course of. GC-clamped amplicons similar to a variable area of the bacterial group 16S rDNA have been synthesized utilizing polymerase chain response (PCR) after which resolved on a base-composition foundation utilizing preparative DHPLC.

Eluate fractions have been collected at random and used as a supply of complete group DNA that may very well be used to find out the bacterial variety. As a primary method, GC-clamps have been faraway from the eluted DNA fragments utilizing PCR to keep away from the doable bias these clamps may trigger through the building of clone libraries. As a second method, a clone library of every eluate pattern was constructed, preserving the GC-clamps of the DNA fragments.

The primary method generated 132 bacterial rDNA sequences with a median measurement of 200 bp, 45% of which had similarity to unculturable or non-classified micro organism. The second method produced 194 sequences recognized as Proteobacteria (48%), uncultured or non-classified environmental micro organism (40%) and Firmicutes (12%). We detected a remarkably larger bacterial variety utilizing the primary method than the second method. The DHPLC-PCR technique allowed for the quick and non-laborious detection of an unlimited bacterial variety that was related to cassava fermentation, and we conclude that it’s a promising various for the characterization of the general microbial variety in complicated samples.

Use of denaturing high-performance liquid chromatography (DHPLC) to characterize the bacterial and fungal airway microbiota of cystic fibrosis sufferers 

The intention of this research was to guage using denaturing high-performance liquid chromatography (DHPLC) to characterize cystic fibrosis (CF) airway microbiota together with each micro organism and fungi. DHPLC circumstances have been first optimized utilizing a mix of V6, V7 and V8 area 16S rRNA gene PCR amplicons from 18 bacterial species generally present in CF sufferers. Then, the microbial variety of four sputum samples from four CF sufferers was analyzed utilizing cultural strategies, cloning/sequencing (for micro organism solely) and DHPLC peak fraction assortment/sequencing. DHPLC evaluation allowed figuring out extra bacterial and fungal species than the classical tradition strategies, together with well-recognized pathogens resembling Pseudomonas aeruginosa.

Even when a decrease variety of bacterial Operational Taxonomic Models (OTUs) was recognized by DHPLC, it allowed to search out OTUs unidentified by cloning/sequencing. The mix of each strategies permitted to correlate the vast majority of DHPLC peaks to outlined OTUs. Lastly, though Aspergillus fumigatus detection utilizing DHPLC can nonetheless be improved, this system clearly allowed to determine a better variety of fungal species versus classical culture-based strategies. To conclude, DHPLC supplied significant extra knowledge regarding pathogenic micro organism and fungi in addition to fastidious microorganisms current throughout the CF respiratory tract. DHPLC may be thought of as a complementary method to culture-dependent analyses in routine microbiological laboratories.

DHPLC and MS research of a photoinduced intrastrand cross-link in DNA labeled with 5-bromo-2′-deoxyuridine.

It’s well-known that the alternative of thymidine with 5-bromo-2′-deoxyuridine (BrdU) in DNA sensitizes it to UVB mild. Irradiation of a biopolymer substituted in such a means results in manifold sorts of DNA harm, resembling intrastrand cross-links, single- and double-strand breaks or alkali-labile websites that have been studied previously with a broad spectrum of analytical strategies. Right here, we show that utterly denaturing high-performance liquid chromatography (DHPLC), underestimated to this point in DNA harm research, may act as a cheap, and high-resolution substitute for the generally employed gel electrophoresis.

We report on the DHPLC/mass spectrometry (MS) analyses of photolytes obtained with the UV irradiation of aqueous options containing 40 base pairs of a protracted, double-stranded oligonucleotide labeled with BrdU in one in all its strands.

The UV-product was detected by HPLC at a temperature of 70°C. Subsequent MS evaluation with electrospray ionization (ESI-MS) of the photolyte, enzymatic digestion of the irradiated materials and HPLC and MS evaluation (LC-MS) of the digest demonstrated unequivocally that an intrastrand covalent dimer, involving adenine and uracil, is shaped within the irradiated system.

CD44 Antigen (CD44) Antibody
20-abx001198	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

CD44 Antigen (CD44) Antibody
abx025270-400ul	Abbexa	400 ul	EUR 523

CD44 Antigen (CD44) Antibody
abx025270-80l	Abbexa	80 µl	EUR 286

CD44 Antigen (CD44) Antibody
abx015801-100ul	Abbexa	100 ul	EUR 411

CD44 Antigen (CD44) Antibody
20-abx019050	Abbexa	EUR 342.00 EUR 77.00	100 ug 5 ug

CD44 Antigen (CD44) Antibody
abx021434-02mg	Abbexa	0.2 mg	EUR 537

CD44 Antigen (CD44) Antibody
abx021435-02mg	Abbexa	0.2 mg	EUR 537

CD44 Antigen (CD44) Antibody
abx412639-025mg	Abbexa	0.25 mg	EUR 565

CD44 Antigen (CD44) Antibody
abx412640-25ug	Abbexa	25 ug	EUR 272

CD44 Antigen (CD44) Antibody
abx412685-01mg	Abbexa	0.1 mg	EUR 453

CD44 Antigen (CD44) Antibody
abx432475-200ul	Abbexa	200 ul	EUR 384

CD44 Antigen (CD44) Antibody
abx412739-01mg	Abbexa	0.1 mg	EUR 453

CD44 Antigen (CD44) Antibody
abx413849-01mg	Abbexa	0.1 mg	EUR 453

CD44 Antigen (CD44) Antibody
abx414285-02mg	Abbexa	0.2 mg	EUR 565

CD44 Antigen (CD44) Antibody
abx414286-25ug	Abbexa	25 ug	EUR 272

CD44 Antigen (CD44) Antibody
abx414525-02mg	Abbexa	0.2 mg	EUR 565

CD44 Antigen (CD44) Antibody
abx414526-25ug	Abbexa	25 ug	EUR 272

CD44 Antigen (CD44) Antibody
abx414875-025mg	Abbexa	0.25 mg	EUR 565

CD44 Antigen (CD44) Antibody
abx415170-025mg	Abbexa	0.25 mg	EUR 592

CD44 Antigen (CD44) Antibody
abx415396-01mg	Abbexa	0.1 mg	EUR 439

CD44 Antigen (CD44) Antibody
abx415398-025mg	Abbexa	0.25 mg	EUR 565

CD44 Antigen (CD44) Antibody
abx415399-1mg	Abbexa	1 mg	EUR 829

CD44 Antigen (CD44) Antibody
abx415545-01mg	Abbexa	0.1 mg	EUR 453

CD44 Antigen (CD44) Antibody
abx415612-02mg	Abbexa	0.2 mg	EUR 565

CD44 Antigen (CD44) Antibody
abx415623-20ug	Abbexa	20 ug	EUR 272

CD44 Antigen (CD44) Antibody
abx231482-100ug	Abbexa	100 ug	EUR 509

CD44 Antigen (CD44) Antibody
abx231483-100ug	Abbexa	100 ug	EUR 481

CD44 Antigen (CD44) Antibody
abx200293-100ug	Abbexa	100 ug	EUR 328

CD44 Antigen (CD44) Antibody
20-abx200798	Abbexa	EUR 272.00 EUR 439.00	100 ug 500 ug

CD44 Antigen (CD44) Antibody
20-abx241364	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

CD44 Antigen (CD44) Antibody
20-abx242943	Abbexa	EUR 467.00 EUR 3042.00 EUR 328.00	100 ul 1 ml 50 ul

CD44 Antigen (CD44) Antibody
20-abx212503	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

CD44 Antigen (CD44) Antibody
abx139276-01mg	Abbexa	0.1 mg	EUR 370

CD44 Antigen (CD44) Antibody
20-abx137492	Abbexa	EUR 704.00 EUR 328.00 EUR 230.00	100 ug 20 ug 5 ug

CD44 Antigen (CD44) Antibody
abx139277-01mg	Abbexa	0.1 mg	EUR 370

CD44 Antigen (CD44) Antibody
20-abx211318	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

CD44 Antigen (CD44) Antibody
abx332801-100ul	Abbexa	100 ul	EUR 425

CD44 Antigen (CD44) Antibody
20-abx327913	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug

CD44 Antigen (CD44) Antibody (FITC)
abx134409-100Assays	Abbexa	100 Assays	EUR 439

CD44 Antigen (CD44) Antibody (FITC)
abx134409-50Assays	Abbexa	50 Assays	EUR 342

CD44 Antigen (CD44) Antibody (PE)
abx134410-100Assays	Abbexa	100 Assays	EUR 495

CD44 Antigen (CD44) Antibody (PE)
abx134410-50Assays	Abbexa	50 Assays	EUR 398

CD44 Antigen (CD44) Antibody (FITC)
abx412636-01mg	Abbexa	0.1 mg	EUR 509

CD44 Antigen (CD44) Antibody (FITC)
abx412637-05mg	Abbexa	0.5 mg	EUR 718

CD44 Antigen (CD44) Antibody (FITC)
abx412638-25ug	Abbexa	25 ug	EUR 272

CD44 Antigen (CD44) Antibody (FITC)
abx412738-01mg	Abbexa	0.1 mg	EUR 648

CD44 Antigen (CD44) Antibody (RPE)
abx412740-100tests	Abbexa	100 tests	EUR 718

CD44 Antigen (CD44) Antibody (FITC)
abx413848-01mg	Abbexa	0.1 mg	EUR 648

CD44 Antigen (CD44) Antibody (FITC)
abx414183-01mg	Abbexa	0.1 mg	EUR 648

CD44 Antigen (CD44) Antibody (FITC)
abx415394-01mg	Abbexa	0.1 mg	EUR 509

CD44 Antigen (CD44) Antibody (FITC)
abx415395-50ug	Abbexa	50 ug	EUR 342

CD44 Antigen (CD44) Antibody (RPE)
abx415397-100tests	Abbexa	100 tests	EUR 592

CD44 Antigen (CD44) Antibody (FITC)
abx415619-01mg	Abbexa	0.1 mg	EUR 509

CD44 Antigen (CD44) Antibody (FITC)
abx415620-25ug	Abbexa	25 ug	EUR 272

CD44 Antigen (CD44) Antibody (RPE)
abx415621-100tests	Abbexa	100 tests	EUR 592

CD44 Antigen (CD44) Antibody (RPE)
abx415622-25tests	Abbexa	25 tests	EUR 314

CD44 Antigen (CD44) Antibody (FITC)
abx200294-100tests	Abbexa	100 tests	EUR 398

CD44 Antigen (CD44) Antibody (PE)
abx200295-100tests	Abbexa	100 tests	EUR 551

CD44 Antigen (CD44) Antibody (APC)
abx200296-100tests	Abbexa	100 tests	EUR 453

CD44 Antigen (CD44) Antibody (PerCP)
abx200297-100tests	Abbexa	100 tests	EUR 718

CD44 Antigen (CD44) Antibody (Biotin)
abx200298-100ug	Abbexa	100 ug	EUR 384

CD44 Antigen (CD44) Antibody (APC)
20-abx200791	Abbexa	EUR 300.00 EUR 217.00	100 ug 25 ug

CD44 Antigen (CD44) Antibody (Biotin)
20-abx200792	Abbexa	EUR 398.00 EUR 230.00	100 ug 25 ug

CD44 Antigen (CD44) Antibody (FITC)
20-abx200794	Abbexa	EUR 300.00 EUR 217.00 EUR 314.00	100 ug 25 ug 500 ug

CD44 Antigen (CD44) Antibody (PE)
20-abx200795	Abbexa	EUR 300.00 EUR 217.00	100 ug 25 ug

CD44 Antigen (CD44) Antibody (PerCP)
20-abx200796	Abbexa	EUR 551.00 EUR 300.00	100 ug 25 ug

CD44 Antigen (CD44) Antibody (PE)
abx139278-100tests	Abbexa	100 tests	EUR 481

CD44 Antigen (CD44) Antibody (APC)
abx139283-100tests	Abbexa	100 tests	EUR 481

CD44 Antigen (CD44) Antibody (FITC)
abx139284-100tests	Abbexa	100 tests	EUR 425

CD44 Antigen (CD44) Antibody (PE)
abx139286-01mg	Abbexa	0.1 mg	EUR 342

CD44 Antigen (CD44) Antibody (Biotin)
abx139288-01mg	Abbexa	0.1 mg	EUR 314

CD44 Antigen (CD44) Antibody (APC)
abx139279-100tests	Abbexa	100 tests	EUR 481

CD44 Antigen (CD44) Antibody (Biotin)
abx139280-01mg	Abbexa	0.1 mg	EUR 425

CD44 Antigen (CD44) Antibody (FITC)
abx139281-100tests	Abbexa	100 tests	EUR 425

CD44 Antigen (CD44) Antibody (PE)
abx139282-100tests	Abbexa	100 tests	EUR 481

Human CD44/ CD44 antigen ELISA Kit
E0426Hu	Sunlong	1 Kit	EUR 563

Human CD44 Antigen (CD44) ELISA Kit
abx253618-96tests	Abbexa	96 tests	EUR 668

Human CD44(CD44 antigen) ELISA Kit
EH0654	FN Test	96T	EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human CD44 antigen, CD44 ELISA KIT
ELI-02342h	Lifescience Market	96 Tests	EUR 824

Dog CD44/ CD44 antigen ELISA Kit
E0019Do	Sunlong	1 Kit	EUR 717

Cow CD44 Antigen (CD44) ELISA Kit
abx513662-96tests	Abbexa	96 tests	EUR 911

Dog CD44 Antigen (CD44) ELISA Kit
abx513663-96tests	Abbexa	96 tests	EUR 911

Rat CD44 Antigen (CD44) ELISA Kit
abx513666-96tests	Abbexa	96 tests	EUR 754

Rat Cd44/ CD44 antigen ELISA Kit
E0180Ra	Sunlong	1 Kit	EUR 571

Mouse Cd44/ CD44 antigen ELISA Kit
E0253Mo	Sunlong	1 Kit	EUR 571

Rat CD44 Antigen (CD44) ELISA Kit
abx256320-96tests	Abbexa	96 tests	EUR 754

Mouse CD44 Antigen (CD44) ELISA Kit
abx254787-96tests	Abbexa	96 tests	EUR 668

CD44 Antigen (CD44) Antibody (CF-Blue)
abx200299-100tests	Abbexa	100 tests	EUR 481

CD44 Antigen (CD44) Antibody (CF-Blue)
20-abx200793	Abbexa	EUR 425.00 EUR 286.00	100 ug 25 ug

CD44 Antigen (CD44) Antibody (PerCP-Cy5.5)
20-abx200797	Abbexa	EUR 495.00 EUR 300.00	100 ug 25 ug