Using COLD-PCR, DHPLC and GeneScanning for the extremely delicate detection of c-KIT somatic mutations in canine mast cell tumours.
The traditional polymerase chain response (PCR)/sequencing strategies could also be poorly suited to the detection of somatic mutations in canine mast cell tumour (MCT) samples owing to restricted sensitivity.
This research was geared toward establishing novel and extra delicate strategies, assessing their restrict of detection and evaluating their sensitivity with standard strategies.Two completely different ‘driver’ somatic mutations of c-KIT, along with the wild-type counterparts, have been cloned in plasmids to organize customary samples with identified concentrations of mutated alleles in a background of wild-type alleles; the plasmids requirements have been assayed utilizing both standard or novel, extremely delicate method.
Standard PCR/sequencing confirmed a sensitivity of 50-20%. Conversely, all of the novel strategies obtained greater sensitivities allowed reaching as little as 2.5-1.2% of the mutated DNA.The research demonstrates that early standard strategies may possible have underestimated the prevalence of KIT mutations of MCTs, subsequently affecting the evaluation of their relevance in prognosis and tyrosine kinase inhibitor (TKI) remedy effectiveness.

des-testbed
Mouse Filaggrin (FLG) ELISA Kit |
DLR-FLG-Mu-48T |
DL Develop |
48T |
EUR 527 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Filaggrin (FLG) in samples from tissue homogenates, cell lysates or other biological fluids. |
Mouse Filaggrin (FLG) ELISA Kit |
DLR-FLG-Mu-96T |
DL Develop |
96T |
EUR 688 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Filaggrin (FLG) in samples from tissue homogenates, cell lysates or other biological fluids. |
Mouse Filaggrin (FLG) ELISA Kit |
RDR-FLG-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 557 |
Mouse Filaggrin (FLG) ELISA Kit |
RDR-FLG-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 774 |
Recombinant Filaggrin (FLG) |
4-RPJ103Hu01 |
Cloud-Clone |
-
EUR 816.80
-
EUR 322.00
-
EUR 2788.00
-
EUR 996.00
-
EUR 1892.00
-
EUR 610.00
-
EUR 6820.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Human Filaggrin expressed in: E.coli |
Recombinant Filaggrin (FLG) |
4-RPJ103Mu01 |
Cloud-Clone |
-
EUR 530.08
-
EUR 245.00
-
EUR 1712.80
-
EUR 637.60
-
EUR 1175.20
-
EUR 418.00
-
EUR 4132.00
|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Mouse Filaggrin expressed in: E.coli |
Human Filaggrin (FLG) |
1-CSB-YP008712HU |
Cusabio |
-
EUR 430.00
-
EUR 234.00
-
EUR 1508.00
-
EUR 642.00
-
EUR 1009.00
-
EUR 291.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Human Filaggrin(FLG),partial expressed in Yeast |
Human Filaggrin (FLG) |
1-CSB-BP008712HU |
Cusabio |
-
EUR 840.00
-
EUR 332.00
-
EUR 2170.00
-
EUR 1135.00
-
EUR 1579.00
-
EUR 470.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Human Filaggrin(FLG) ,partial expressed in Baculovirus |
Human Filaggrin (FLG) |
1-CSB-EP008712HU |
Cusabio |
-
EUR 380.00
-
EUR 214.00
-
EUR 1309.00
-
EUR 560.00
-
EUR 873.00
-
EUR 262.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Human Filaggrin(FLG),partial expressed in E.coli |
Filaggrin (FLG) Antibody |
20-abx109789 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Filaggrin (FLG) Antibody |
20-abx339302 |
Abbexa |
|
|
|
Filaggrin (FLG) Antibody |
20-abx339766 |
Abbexa |
|
|
|
Filaggrin (FLG) Antibody |
20-abx100249 |
Abbexa |
-
EUR 425.00
-
EUR 133.00
-
EUR 1205.00
-
EUR 578.00
-
EUR 328.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Filaggrin (FLG) Antibody |
20-abx100250 |
Abbexa |
-
EUR 439.00
-
EUR 133.00
-
EUR 1233.00
-
EUR 592.00
-
EUR 328.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Filaggrin (FLG) Antibody |
20-abx133148 |
Abbexa |
-
EUR 300.00
-
EUR 439.00
-
EUR 189.00
|
|
|
Filaggrin (FLG) Antibody |
20-abx176473 |
Abbexa |
|
|
|
Filaggrin (FLG) Antibody |
20-abx172440 |
Abbexa |
|
|
|
Human Filaggrin (FLG) Protein |
20-abx066657 |
Abbexa |
-
EUR 1121.00
-
EUR 411.00
-
EUR 3724.00
-
EUR 1358.00
-
EUR 759.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Anti-Filaggrin/FLG Antibody |
PB9870 |
BosterBio |
100ug/vial |
EUR 334 |
Filaggrin (FLG) Antibody (FITC) |
20-abx106841 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Filaggrin (FLG) Antibody (HRP) |
20-abx108261 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Filaggrin (FLG) Antibody (Biotin) |
20-abx105422 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Mouse Filaggrin (FLG) Protein |
20-abx066658 |
Abbexa |
-
EUR 732.00
-
EUR 286.00
-
EUR 2305.00
-
EUR 885.00
-
EUR 523.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Filaggrin (FLG) Antibody (FITC) |
20-abx271155 |
Abbexa |
-
EUR 495.00
-
EUR 258.00
-
EUR 1455.00
-
EUR 676.00
-
EUR 398.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Filaggrin (FLG) Antibody (Biotin) |
20-abx271421 |
Abbexa |
-
EUR 467.00
-
EUR 244.00
-
EUR 1344.00
-
EUR 634.00
-
EUR 342.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Filaggrin (FLG) Antibody (Biotin) |
20-abx272419 |
Abbexa |
-
EUR 453.00
-
EUR 244.00
-
EUR 1316.00
-
EUR 620.00
-
EUR 342.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Filaggrin (FLG) Antibody (FITC) |
20-abx273568 |
Abbexa |
-
EUR 481.00
-
EUR 244.00
-
EUR 1414.00
-
EUR 662.00
-
EUR 356.00
|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Human Filaggrin (FLG)CLIA Kit |
SCJ103Hu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 5647.8 |
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Filaggrin (FLG) in Tissue homogenates and other biological fluids. |
Human Filaggrin (FLG)CLIA Kit |
SCJ103Hu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 552.76 |
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Filaggrin (FLG) in Tissue homogenates and other biological fluids. |
Human Filaggrin (FLG)CLIA Kit |
SCJ103Hu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 746.8 |
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Filaggrin (FLG) in Tissue homogenates and other biological fluids. |
Human Filaggrin (FLG)CLIA Kit |
SCJ103Hu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 3060.6 |
|
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Filaggrin (FLG) in Tissue homogenates and other biological fluids. |
Human Filaggrin (FLG) CLIA Kit |
4-SCJ103Hu |
Cloud-Clone |
-
EUR 5698.00
-
EUR 3061.00
-
EUR 747.00
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Filaggrin (FLG)Tissue homogenates and other biological fluids |
Filaggrin (FLG) Polyclonal Antibody (Human) |
4-PAJ103Hu01 |
Cloud-Clone |
-
EUR 247.00
-
EUR 2510.00
-
EUR 625.00
-
EUR 310.00
-
EUR 214.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG) |
Human Filaggrin (FLG) ELISA Kit |
SEJ103Hu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 4731.5 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Filaggrin (FLG) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Human Filaggrin (FLG) ELISA Kit |
SEJ103Hu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 477.3 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Filaggrin (FLG) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Human Filaggrin (FLG) ELISA Kit |
SEJ103Hu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 639 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Filaggrin (FLG) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Human Filaggrin (FLG) ELISA Kit |
SEJ103Hu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2575.5 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Filaggrin (FLG) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Human Filaggrin (FLG) ELISA Kit |
4-SEJ103Hu |
Cloud-Clone |
-
EUR 4782.00
-
EUR 2526.00
-
EUR 640.00
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Filaggrin (FLG) in samples from tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. |
Human Filaggrin(FLG) ELISA kit |
CSB-EL008712HU-24T |
Cusabio |
1 plate of 24 wells |
EUR 165 |
|
Description: Quantitativesandwich ELISA kit for measuring Human Filaggrin (FLG) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Human Filaggrin(FLG) ELISA kit |
1-CSB-EL008712HU |
Cusabio |
-
EUR 804.00
-
EUR 5099.00
-
EUR 2704.00
|
- 1 plate of 96 wells
- 10 plates of 96 wells each
- 5 plates of 96 wells each
|
|
Description: Quantitativesandwich ELISA kit for measuring Human Filaggrin(FLG) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Human FLG/ Filaggrin ELISA Kit |
E0920Hu |
Sunlong |
1 Kit |
EUR 571 |
Human Filaggrin (FLG)ELISA Kit |
201-12-2700 |
SunredBio |
96 tests |
EUR 440 |
|
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human Filaggrin (FLG) CLIA Kit |
20-abx190272 |
Abbexa |
-
EUR 7911.00
-
EUR 4215.00
-
EUR 973.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Human Filaggrin (FLG) ELISA Kit |
abx572526-96tests |
Abbexa |
96 tests |
EUR 668 |
|
Human Filaggrin (FLG) ELISA Kit |
20-abx151555 |
Abbexa |
-
EUR 7378.00
-
EUR 3933.00
-
EUR 911.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Human Filaggrin ELISA Kit (FLG) |
RK01403 |
Abclonal |
96 Tests |
EUR 521 |
Human FLG(Filaggrin) ELISA Kit |
EH1353 |
FN Test |
96T |
EUR 567.6 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
Filaggrin (FLG) Polyclonal Antibody (Human), APC |
4-PAJ103Hu01-APC |
Cloud-Clone |
-
EUR 345.00
-
EUR 3275.00
-
EUR 912.00
-
EUR 440.00
-
EUR 219.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with APC. |
Filaggrin (FLG) Polyclonal Antibody (Human), Biotinylated |
4-PAJ103Hu01-Biotin |
Cloud-Clone |
-
EUR 311.00
-
EUR 2460.00
-
EUR 727.00
-
EUR 381.00
-
EUR 219.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with Biotin. |
Filaggrin (FLG) Polyclonal Antibody (Human), Cy3 |
4-PAJ103Hu01-Cy3 |
Cloud-Clone |
-
EUR 419.00
-
EUR 4325.00
-
EUR 1175.00
-
EUR 545.00
-
EUR 251.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with Cy3. |
Filaggrin (FLG) Polyclonal Antibody (Human), FITC |
4-PAJ103Hu01-FITC |
Cloud-Clone |
-
EUR 296.00
-
EUR 2640.00
-
EUR 750.00
-
EUR 372.00
-
EUR 195.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with FITC. |
Filaggrin (FLG) Polyclonal Antibody (Human), HRP |
4-PAJ103Hu01-HRP |
Cloud-Clone |
-
EUR 316.00
-
EUR 2855.00
-
EUR 807.00
-
EUR 398.00
-
EUR 206.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with HRP. |
Filaggrin (FLG) Polyclonal Antibody (Human), PE |
4-PAJ103Hu01-PE |
Cloud-Clone |
-
EUR 296.00
-
EUR 2640.00
-
EUR 750.00
-
EUR 372.00
-
EUR 195.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with PE. |
ELISA kit for Human FLG (Filaggrin) |
ELK3513 |
ELK Biotech |
1 plate of 96 wells |
EUR 432 |
|
Description: A sandwich ELISA kit for detection of Filaggrin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
Mouse Filaggrin (FLG) ELISA Kit |
SEJ103Mu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 4862.4 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Filaggrin (FLG) in tissue homogenates, cell lysates and other biological fluids. |
Mouse Filaggrin (FLG) ELISA Kit |
SEJ103Mu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 488.08 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Filaggrin (FLG) in tissue homogenates, cell lysates and other biological fluids. |
Mouse Filaggrin (FLG) ELISA Kit |
SEJ103Mu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 654.4 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Filaggrin (FLG) in tissue homogenates, cell lysates and other biological fluids. |
Mouse Filaggrin (FLG) ELISA Kit |
SEJ103Mu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2644.8 |
|
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Filaggrin (FLG) in tissue homogenates, cell lysates and other biological fluids. |
Mouse Filaggrin (FLG) ELISA Kit |
4-SEJ103Mu |
Cloud-Clone |
-
EUR 4913.00
-
EUR 2595.00
-
EUR 655.00
|
- 10 plates of 96 wells
- 5 plates of 96 wells
- 1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Filaggrin (FLG) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. |
Mouse Filaggrin(FLG) ELISA kit |
CSB-EL008712MO-24T |
Cusabio |
1 plate of 24 wells |
EUR 165 |
|
Description: Quantitativesandwich ELISA kit for measuring Mouse Filaggrin (FLG) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Mouse Filaggrin(FLG) ELISA kit |
1-CSB-EL008712MO |
Cusabio |
-
EUR 804.00
-
EUR 5099.00
-
EUR 2704.00
|
- 1 plate of 96 wells
- 10 plates of 96 wells each
- 5 plates of 96 wells each
|
|
Description: Quantitativesandwich ELISA kit for measuring Mouse Filaggrin(FLG) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Mouse Filaggrin ELISA Kit (FLG) |
RK02813 |
Abclonal |
96 Tests |
EUR 521 |
Mouse Flg/ Filaggrin ELISA Kit |
E0543Mo |
Sunlong |
1 Kit |
EUR 571 |
Mouse Filaggrin (FLG) ELISA Kit |
abx573034-96tests |
Abbexa |
96 tests |
EUR 668 |
|
Mouse Filaggrin (FLG) CLIA Kit |
20-abx495617 |
Abbexa |
-
EUR 7973.00
-
EUR 4246.00
-
EUR 981.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Mouse Filaggrin (FLG) ELISA Kit |
20-abx154027 |
Abbexa |
-
EUR 7378.00
-
EUR 3933.00
-
EUR 911.00
|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Mouse Filaggrin (FLG) ELISA Kit |
abx257768-96tests |
Abbexa |
96 tests |
EUR 746 |
|
Mouse FLG(Filaggrin) ELISA Kit |
EM1853 |
FN Test |
96T |
EUR 567.6 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 9.375pg/ml |
Filaggrin (FLG) Polyclonal Antibody (Human), APC-Cy7 |
4-PAJ103Hu01-APC-Cy7 |
Cloud-Clone |
-
EUR 571.00
-
EUR 6430.00
-
EUR 1705.00
-
EUR 760.00
-
EUR 319.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Human Filaggrin (FLG). This antibody is labeled with APC-Cy7. |
Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat) |
4-PAJ103Mu01 |
Cloud-Clone |
-
EUR 251.00
-
EUR 2576.00
-
EUR 640.00
-
EUR 316.00
-
EUR 215.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG) |
ELISA kit for Mouse FLG (Filaggrin) |
ELK6992 |
ELK Biotech |
1 plate of 96 wells |
EUR 432 |
|
Description: A sandwich ELISA kit for detection of Filaggrin from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
ELISA kit for Human FLG-Ab (Anti-Filaggrin) |
E-EL-H0283 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 534 |
|
Description: A sandwich ELISA kit for quantitative measurement of Human FLG-Ab (Anti-Filaggrin) in samples from Serum, Plasma, Cell supernatant |
Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), APC |
4-PAJ103Mu01-APC |
Cloud-Clone |
-
EUR 351.00
-
EUR 3365.00
-
EUR 935.00
-
EUR 449.00
-
EUR 222.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with APC. |
Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), Biotinylated |
4-PAJ103Mu01-Biotin |
Cloud-Clone |
-
EUR 316.00
-
EUR 2526.00
-
EUR 744.00
-
EUR 387.00
-
EUR 221.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with Biotin. |
Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), Cy3 |
4-PAJ103Mu01-Cy3 |
Cloud-Clone |
-
EUR 427.00
-
EUR 4445.00
-
EUR 1205.00
-
EUR 557.00
-
EUR 254.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with Cy3. |
Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), FITC |
4-PAJ103Mu01-FITC |
Cloud-Clone |
-
EUR 301.00
-
EUR 2712.00
-
EUR 768.00
-
EUR 379.00
-
EUR 197.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with FITC. |
Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), HRP |
4-PAJ103Mu01-HRP |
Cloud-Clone |
-
EUR 321.00
-
EUR 2933.00
-
EUR 827.00
-
EUR 405.00
-
EUR 209.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with HRP. |
Filaggrin (FLG) Polyclonal Antibody (Mouse, Rat), PE |
4-PAJ103Mu01-PE |
Cloud-Clone |
-
EUR 301.00
-
EUR 2712.00
-
EUR 768.00
-
EUR 379.00
-
EUR 197.00
|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against Mouse, Rat Filaggrin (FLG). This antibody is labeled with PE. |
Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561) Antibody |
BNC041561-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561),CF405S conjugate, Concentration: 0.1mg/mL |
Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561) Antibody |
BNC041561-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1561),CF405S conjugate, Concentration: 0.1mg/mL |
Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562) Antibody |
BNC041562-100 |
Biotium |
100uL |
EUR 199 |
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562),CF405S conjugate, Concentration: 0.1mg/mL |
Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562) Antibody |
BNC041562-500 |
Biotium |
500uL |
EUR 544 |
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1562),CF405S conjugate, Concentration: 0.1mg/mL |
Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563) Antibody |
BNC041563-100 |
Biotium |
100uL |
EUR 233 |
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563), CF405S conjugate, Concentration: 0.1mg/mL |
Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563) Antibody |
BNC041563-500 |
Biotium |
500uL |
EUR 545 |
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1563), CF405S conjugate, Concentration: 0.1mg/mL |
Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945) Antibody |
BNC041945-100 |
Biotium |
100uL |
EUR 233 |
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945), CF405S conjugate, Concentration: 0.1mg/mL |
Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945) Antibody |
BNC041945-500 |
Biotium |
500uL |
EUR 545 |
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1945), CF405S conjugate, Concentration: 0.1mg/mL |
Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R) Antibody |
BNC041957-100 |
Biotium |
100uL |
EUR 233 |
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R), CF405S conjugate, Concentration: 0.1mg/mL |
Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R) Antibody |
BNC041957-500 |
Biotium |
500uL |
EUR 545 |
Description: Primary antibody against Filaggrin (Keratinocyte Differentiation Marker) (FLG/1957R), CF405S conjugate, Concentration: 0.1mg/mL |
Environment friendly IDUA Gene Mutation Detection with Mixed Use of dHPLC and Dried Blood Samples
Aims. Growth of a easy mutation directed technique with a view to enable reducing the price of mutation testing utilizing an simply obtainable organic materials. Evaluation of the feasibility of such technique was examined utilizing a GC-rich amplicon. Design and Strategies. A way of denaturing high-performance liquid chromatography (dHPLC) was improved and applied as a method for the detection of variants in exon 9 of the IDUA gene. The optimized technique was examined in 500 genomic DNA samples obtained from dried blood spots (DBS).
Outcomes. With this dHPLC method it was doable to detect completely different variants, together with the widespread p.Trp402Ter mutation within the IDUA gene. The excessive GC content material didn’t intervene with the decision and reliability of this system, and discrimination of G-C transversions was additionally achieved. Conclusion. This PCR-based dHPLC technique is proved to be a fast, a delicate, and a very good possibility for screening quite a few samples obtained from DBS. Moreover, it resulted within the constant detection of clearly distinguishable profiles of the widespread p.Trp402Ter IDUA mutation with an advantageous stability of value and technical necessities.
Use of PCR-DHPLC with fluorescence detection for the characterization of the bacterial variety throughout cassava (Manihot esculenta Crantz) fermentation
Denaturing high-performance liquid chromatography (DHPLC) has been described as an appropriate technique to check DNA polymorphisms. Right here, cassava (Manihot esculenta Crantz) fermentation liquor was examined utilizing DHPLC evaluation to characterize the bacterial variety through the fermentation course of. GC-clamped amplicons similar to a variable area of the bacterial group 16S rDNA have been synthesized utilizing polymerase chain response (PCR) after which resolved on a base-composition foundation utilizing preparative DHPLC.
Eluate fractions have been collected at random and used as a supply of complete group DNA that may very well be used to find out the bacterial variety. As a primary method, GC-clamps have been faraway from the eluted DNA fragments utilizing PCR to keep away from the doable bias these clamps may trigger through the building of clone libraries. As a second method, a clone library of every eluate pattern was constructed, preserving the GC-clamps of the DNA fragments.
The primary method generated 132 bacterial rDNA sequences with a median measurement of 200 bp, 45% of which had similarity to unculturable or non-classified micro organism. The second method produced 194 sequences recognized as Proteobacteria (48%), uncultured or non-classified environmental micro organism (40%) and Firmicutes (12%). We detected a remarkably larger bacterial variety utilizing the primary method than the second method. The DHPLC-PCR technique allowed for the quick and non-laborious detection of an unlimited bacterial variety that was related to cassava fermentation, and we conclude that it’s a promising various for the characterization of the general microbial variety in complicated samples.
Use of denaturing high-performance liquid chromatography (DHPLC) to characterize the bacterial and fungal airway microbiota of cystic fibrosis sufferers
The intention of this research was to guage using denaturing high-performance liquid chromatography (DHPLC) to characterize cystic fibrosis (CF) airway microbiota together with each micro organism and fungi. DHPLC circumstances have been first optimized utilizing a mix of V6, V7 and V8 area 16S rRNA gene PCR amplicons from 18 bacterial species generally present in CF sufferers. Then, the microbial variety of four sputum samples from four CF sufferers was analyzed utilizing cultural strategies, cloning/sequencing (for micro organism solely) and DHPLC peak fraction assortment/sequencing. DHPLC evaluation allowed figuring out extra bacterial and fungal species than the classical tradition strategies, together with well-recognized pathogens resembling Pseudomonas aeruginosa.
Even when a decrease variety of bacterial Operational Taxonomic Models (OTUs) was recognized by DHPLC, it allowed to search out OTUs unidentified by cloning/sequencing. The mix of each strategies permitted to correlate the vast majority of DHPLC peaks to outlined OTUs. Lastly, though Aspergillus fumigatus detection utilizing DHPLC can nonetheless be improved, this system clearly allowed to determine a better variety of fungal species versus classical culture-based strategies. To conclude, DHPLC supplied significant extra knowledge regarding pathogenic micro organism and fungi in addition to fastidious microorganisms current throughout the CF respiratory tract. DHPLC may be thought of as a complementary method to culture-dependent analyses in routine microbiological laboratories.
DHPLC and MS research of a photoinduced intrastrand cross-link in DNA labeled with 5-bromo-2′-deoxyuridine.
It’s well-known that the alternative of thymidine with 5-bromo-2′-deoxyuridine (BrdU) in DNA sensitizes it to UVB mild. Irradiation of a biopolymer substituted in such a means results in manifold sorts of DNA harm, resembling intrastrand cross-links, single- and double-strand breaks or alkali-labile websites that have been studied previously with a broad spectrum of analytical strategies. Right here, we show that utterly denaturing high-performance liquid chromatography (DHPLC), underestimated to this point in DNA harm research, may act as a cheap, and high-resolution substitute for the generally employed gel electrophoresis.
We report on the DHPLC/mass spectrometry (MS) analyses of photolytes obtained with the UV irradiation of aqueous options containing 40 base pairs of a protracted, double-stranded oligonucleotide labeled with BrdU in one in all its strands.
The UV-product was detected by HPLC at a temperature of 70°C. Subsequent MS evaluation with electrospray ionization (ESI-MS) of the photolyte, enzymatic digestion of the irradiated materials and HPLC and MS evaluation (LC-MS) of the digest demonstrated unequivocally that an intrastrand covalent dimer, involving adenine and uracil, is shaped within the irradiated system.
CD44 Antigen (CD44) Antibody |
20-abx001198 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
CD44 Antigen (CD44) Antibody |
abx025270-400ul |
Abbexa |
400 ul |
EUR 523 |
|
CD44 Antigen (CD44) Antibody |
abx025270-80l |
Abbexa |
80 µl |
EUR 286 |
|
CD44 Antigen (CD44) Antibody |
abx015801-100ul |
Abbexa |
100 ul |
EUR 411 |
|
CD44 Antigen (CD44) Antibody |
20-abx019050 |
Abbexa |
|
|
|
CD44 Antigen (CD44) Antibody |
abx021434-02mg |
Abbexa |
0.2 mg |
EUR 537 |
|
CD44 Antigen (CD44) Antibody |
abx021435-02mg |
Abbexa |
0.2 mg |
EUR 537 |
|
CD44 Antigen (CD44) Antibody |
abx412639-025mg |
Abbexa |
0.25 mg |
EUR 565 |
|
CD44 Antigen (CD44) Antibody |
abx412640-25ug |
Abbexa |
25 ug |
EUR 272 |
|
CD44 Antigen (CD44) Antibody |
abx412685-01mg |
Abbexa |
0.1 mg |
EUR 453 |
|
CD44 Antigen (CD44) Antibody |
abx432475-200ul |
Abbexa |
200 ul |
EUR 384 |
|
CD44 Antigen (CD44) Antibody |
abx412739-01mg |
Abbexa |
0.1 mg |
EUR 453 |
|
CD44 Antigen (CD44) Antibody |
abx413849-01mg |
Abbexa |
0.1 mg |
EUR 453 |
|
CD44 Antigen (CD44) Antibody |
abx414285-02mg |
Abbexa |
0.2 mg |
EUR 565 |
|
CD44 Antigen (CD44) Antibody |
abx414286-25ug |
Abbexa |
25 ug |
EUR 272 |
|
CD44 Antigen (CD44) Antibody |
abx414525-02mg |
Abbexa |
0.2 mg |
EUR 565 |
|
CD44 Antigen (CD44) Antibody |
abx414526-25ug |
Abbexa |
25 ug |
EUR 272 |
|
CD44 Antigen (CD44) Antibody |
abx414875-025mg |
Abbexa |
0.25 mg |
EUR 565 |
|
CD44 Antigen (CD44) Antibody |
abx415170-025mg |
Abbexa |
0.25 mg |
EUR 592 |
|
CD44 Antigen (CD44) Antibody |
abx415396-01mg |
Abbexa |
0.1 mg |
EUR 439 |
|
CD44 Antigen (CD44) Antibody |
abx415398-025mg |
Abbexa |
0.25 mg |
EUR 565 |
|
CD44 Antigen (CD44) Antibody |
abx415399-1mg |
Abbexa |
1 mg |
EUR 829 |
|
CD44 Antigen (CD44) Antibody |
abx415545-01mg |
Abbexa |
0.1 mg |
EUR 453 |
|
CD44 Antigen (CD44) Antibody |
abx415612-02mg |
Abbexa |
0.2 mg |
EUR 565 |
|
CD44 Antigen (CD44) Antibody |
abx415623-20ug |
Abbexa |
20 ug |
EUR 272 |
|
CD44 Antigen (CD44) Antibody |
abx231482-100ug |
Abbexa |
100 ug |
EUR 509 |
|
CD44 Antigen (CD44) Antibody |
abx231483-100ug |
Abbexa |
100 ug |
EUR 481 |
|
CD44 Antigen (CD44) Antibody |
abx200293-100ug |
Abbexa |
100 ug |
EUR 328 |
|
CD44 Antigen (CD44) Antibody |
20-abx200798 |
Abbexa |
|
|
|
CD44 Antigen (CD44) Antibody |
20-abx241364 |
Abbexa |
|
|
|
CD44 Antigen (CD44) Antibody |
20-abx242943 |
Abbexa |
-
EUR 467.00
-
EUR 3042.00
-
EUR 328.00
|
|
|
CD44 Antigen (CD44) Antibody |
20-abx212503 |
Abbexa |
|
|
|
CD44 Antigen (CD44) Antibody |
abx139276-01mg |
Abbexa |
0.1 mg |
EUR 370 |
|
CD44 Antigen (CD44) Antibody |
20-abx137492 |
Abbexa |
-
EUR 704.00
-
EUR 328.00
-
EUR 230.00
|
|
|
CD44 Antigen (CD44) Antibody |
abx139277-01mg |
Abbexa |
0.1 mg |
EUR 370 |
|
CD44 Antigen (CD44) Antibody |
20-abx211318 |
Abbexa |
|
|
|
CD44 Antigen (CD44) Antibody |
abx332801-100ul |
Abbexa |
100 ul |
EUR 425 |
|
CD44 Antigen (CD44) Antibody |
20-abx327913 |
Abbexa |
|
|
|
CD44 Antigen (CD44) Antibody (FITC) |
abx134409-100Assays |
Abbexa |
100 Assays |
EUR 439 |
|
CD44 Antigen (CD44) Antibody (FITC) |
abx134409-50Assays |
Abbexa |
50 Assays |
EUR 342 |
|
CD44 Antigen (CD44) Antibody (PE) |
abx134410-100Assays |
Abbexa |
100 Assays |
EUR 495 |
|
CD44 Antigen (CD44) Antibody (PE) |
abx134410-50Assays |
Abbexa |
50 Assays |
EUR 398 |
|
CD44 Antigen (CD44) Antibody (FITC) |
abx412636-01mg |
Abbexa |
0.1 mg |
EUR 509 |
|
CD44 Antigen (CD44) Antibody (FITC) |
abx412637-05mg |
Abbexa |
0.5 mg |
EUR 718 |
|
CD44 Antigen (CD44) Antibody (FITC) |
abx412638-25ug |
Abbexa |
25 ug |
EUR 272 |
|
CD44 Antigen (CD44) Antibody (FITC) |
abx412738-01mg |
Abbexa |
0.1 mg |
EUR 648 |
|
CD44 Antigen (CD44) Antibody (RPE) |
abx412740-100tests |
Abbexa |
100 tests |
EUR 718 |
|
CD44 Antigen (CD44) Antibody (FITC) |
abx413848-01mg |
Abbexa |
0.1 mg |
EUR 648 |
|
CD44 Antigen (CD44) Antibody (FITC) |
abx414183-01mg |
Abbexa |
0.1 mg |
EUR 648 |
|
CD44 Antigen (CD44) Antibody (FITC) |
abx415394-01mg |
Abbexa |
0.1 mg |
EUR 509 |
|
CD44 Antigen (CD44) Antibody (FITC) |
abx415395-50ug |
Abbexa |
50 ug |
EUR 342 |
|
CD44 Antigen (CD44) Antibody (RPE) |
abx415397-100tests |
Abbexa |
100 tests |
EUR 592 |
|
CD44 Antigen (CD44) Antibody (FITC) |
abx415619-01mg |
Abbexa |
0.1 mg |
EUR 509 |
|
CD44 Antigen (CD44) Antibody (FITC) |
abx415620-25ug |
Abbexa |
25 ug |
EUR 272 |
|
CD44 Antigen (CD44) Antibody (RPE) |
abx415621-100tests |
Abbexa |
100 tests |
EUR 592 |
|
CD44 Antigen (CD44) Antibody (RPE) |
abx415622-25tests |
Abbexa |
25 tests |
EUR 314 |
|
CD44 Antigen (CD44) Antibody (FITC) |
abx200294-100tests |
Abbexa |
100 tests |
EUR 398 |
|
CD44 Antigen (CD44) Antibody (PE) |
abx200295-100tests |
Abbexa |
100 tests |
EUR 551 |
|
CD44 Antigen (CD44) Antibody (APC) |
abx200296-100tests |
Abbexa |
100 tests |
EUR 453 |
|
CD44 Antigen (CD44) Antibody (PerCP) |
abx200297-100tests |
Abbexa |
100 tests |
EUR 718 |
|
CD44 Antigen (CD44) Antibody (Biotin) |
abx200298-100ug |
Abbexa |
100 ug |
EUR 384 |
|
CD44 Antigen (CD44) Antibody (APC) |
20-abx200791 |
Abbexa |
|
|
|
CD44 Antigen (CD44) Antibody (Biotin) |
20-abx200792 |
Abbexa |
|
|
|
CD44 Antigen (CD44) Antibody (FITC) |
20-abx200794 |
Abbexa |
-
EUR 300.00
-
EUR 217.00
-
EUR 314.00
|
|
|
CD44 Antigen (CD44) Antibody (PE) |
20-abx200795 |
Abbexa |
|
|
|
CD44 Antigen (CD44) Antibody (PerCP) |
20-abx200796 |
Abbexa |
|
|
|
CD44 Antigen (CD44) Antibody (PE) |
abx139278-100tests |
Abbexa |
100 tests |
EUR 481 |
|
CD44 Antigen (CD44) Antibody (APC) |
abx139283-100tests |
Abbexa |
100 tests |
EUR 481 |
|
CD44 Antigen (CD44) Antibody (FITC) |
abx139284-100tests |
Abbexa |
100 tests |
EUR 425 |
|
CD44 Antigen (CD44) Antibody (PE) |
abx139286-01mg |
Abbexa |
0.1 mg |
EUR 342 |
|
CD44 Antigen (CD44) Antibody (Biotin) |
abx139288-01mg |
Abbexa |
0.1 mg |
EUR 314 |
|
CD44 Antigen (CD44) Antibody (APC) |
abx139279-100tests |
Abbexa |
100 tests |
EUR 481 |
|
CD44 Antigen (CD44) Antibody (Biotin) |
abx139280-01mg |
Abbexa |
0.1 mg |
EUR 425 |
|
CD44 Antigen (CD44) Antibody (FITC) |
abx139281-100tests |
Abbexa |
100 tests |
EUR 425 |
|
CD44 Antigen (CD44) Antibody (PE) |
abx139282-100tests |
Abbexa |
100 tests |
EUR 481 |
|
Human CD44/ CD44 antigen ELISA Kit |
E0426Hu |
Sunlong |
1 Kit |
EUR 563 |
Human CD44 Antigen (CD44) ELISA Kit |
abx253618-96tests |
Abbexa |
96 tests |
EUR 668 |
|
Human CD44(CD44 antigen) ELISA Kit |
EH0654 |
FN Test |
96T |
EUR 567.6 |
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
Dog CD44/ CD44 antigen ELISA Kit |
E0019Do |
Sunlong |
1 Kit |
EUR 717 |
Cow CD44 Antigen (CD44) ELISA Kit |
abx513662-96tests |
Abbexa |
96 tests |
EUR 911 |
|
Dog CD44 Antigen (CD44) ELISA Kit |
abx513663-96tests |
Abbexa |
96 tests |
EUR 911 |
|
Rat CD44 Antigen (CD44) ELISA Kit |
abx513666-96tests |
Abbexa |
96 tests |
EUR 754 |
|
Rat Cd44/ CD44 antigen ELISA Kit |
E0180Ra |
Sunlong |
1 Kit |
EUR 571 |
Mouse Cd44/ CD44 antigen ELISA Kit |
E0253Mo |
Sunlong |
1 Kit |
EUR 571 |
Rat CD44 Antigen (CD44) ELISA Kit |
abx256320-96tests |
Abbexa |
96 tests |
EUR 754 |
|
Mouse CD44 Antigen (CD44) ELISA Kit |
abx254787-96tests |
Abbexa |
96 tests |
EUR 668 |
|
CD44 Antigen (CD44) Antibody (CF-Blue) |
abx200299-100tests |
Abbexa |
100 tests |
EUR 481 |
|
CD44 Antigen (CD44) Antibody (CF-Blue) |
20-abx200793 |
Abbexa |
|
|
|
CD44 Antigen (CD44) Antibody (PerCP-Cy5.5) |
20-abx200797 |
Abbexa |
|
|
|