DHPLC/SURVEYOR nuclease: a delicate, speedy and inexpensive technique to research BRCA1 and BRCA2 mutations in breast most cancers households.
Hereditary breast most cancers accounts for about 10% of all breast cancers and BRCA1 and BRCA2 genes have been recognized as validated susceptibility genes for this pathology. Testing for BRCA gene mutations is normally based mostly on a pre-screening strategy, such because the partial denaturation DHPLC technique, and capillary direct sequencing. Nevertheless, this strategy is time consuming as a result of massive measurement of BRCA1 and BRCA2 genes. Just lately, a brand new low price and time saving DHPLC protocol has been developed to research gene mutations through the use of SURVEYOR(®) Nuclease digestion and DHPLC evaluation. A subset of 90 sufferers, enrolled within the Genetic Counseling Program of the Nationwide Most cancers Centre of Bari (Italy), was carried out to validate this strategy.
Earlier retrospective evaluation confirmed that 9/90 sufferers (10%) had been mutated in BRCA1 and BRCA2 genes and these information had been confirmed by the current strategy. DNA samples underwent landing PCR and, subsequently, SURVEYOR(®) nuclease digestion. BRCA1 and BRCA2 amplicons had been divided into teams relying on amplicon measurement to permit multiamplicon digestion. The product of this response had been analyzed on Transgenomic WAVE Nucleic Acid Excessive Sensitivity Fragment Evaluation System. The operator who carried out the DHPLC surveyor strategy didn’t know the sequencing outcomes at the moment. The SURVEYOR(®) Nuclease DHPLC strategy was in a position to detect all alterations with a sensitivity of 95%. Moreover, with a purpose to save time and reagents, a multiamplicon setting preparation was validated.
des-testbed
 , partial) Recombinant Human Myosin-1 (MYH1) , partial |
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MBS964626-002mgYeast |
MyBiosource |
0.02mg(Yeast) |
EUR 880 |
Recombinant Human Myosin-1 (MYH1) , partial |
|
MBS964626-01mgEColi |
MyBiosource |
0.1mg(E-Coli) |
EUR 855 |
Recombinant Human Myosin-1 (MYH1) , partial |
|
MBS964626-01mgYeast |
MyBiosource |
0.1mg(Yeast) |
EUR 1035 |
, partial) Recombinant Pig Myosin-1 (MYH1), partial |
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MBS1462893-INQUIRE |
MyBiosource |
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 , partial) Recombinant Mouse Myosin-1 (Myh1) , partial |
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MBS1477688-INQUIRE |
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, partial) Recombinant Horse Myosin-1 (MYH1), partial |
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MBS1466320-INQUIRE |
MyBiosource |
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, partial) Recombinant Bovine Myosin-1 (MYH1), partial |
|
CSB-EP858986BO |
Cusabio |
10533 mg |
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Recombinant Bovine Myosin-1 (MYH1), partial |
|
CSB-EP858986BO-100ug |
Cusabio |
100ug |
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|
|
|
Description: 585-794aa |
Recombinant Bovine Myosin-1 (MYH1), partial |
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CSB-EP858986BO-1mg |
Cusabio |
1mg |
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|
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Description: 585-794aa |
Recombinant Bovine Myosin-1 (MYH1), partial |
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CSB-EP858986BO-20ug |
Cusabio |
20ug |
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|
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Description: 585-794aa |
 , partial) Recombinant Bovine Myosin-1 (MYH1) , partial |
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MBS1458010-INQUIRE |
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 , partial) Recombinant Human Myosin-14 (MYH14) , partial |
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MBS1294964-002mgBaculovirus |
MyBiosource |
0.02mg(Baculovirus) |
EUR 1645 |
Recombinant Human Myosin-14 (MYH14) , partial |
|
MBS1294964-002mgEColi |
MyBiosource |
0.02mg(E-Coli) |
EUR 1390 |
Recombinant Human Myosin-14 (MYH14) , partial |
|
MBS1294964-002mgYeast |
MyBiosource |
0.02mg(Yeast) |
EUR 1405 |
Recombinant Human Myosin-14 (MYH14) , partial |
|
MBS1294964-01mgEColi |
MyBiosource |
0.1mg(E-Coli) |
EUR 1675 |
Recombinant Human Myosin-14 (MYH14) , partial |
|
MBS1294964-01mgYeast |
MyBiosource |
0.1mg(Yeast) |
EUR 1650 |
 , partial) Recombinant Human Myosin-13 (MYH13) , partial |
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MBS1301859-INQUIRE |
MyBiosource |
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 , partial) Recombinant Human Myosin-15 (MYH15) , partial |
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MBS1378539-INQUIRE |
MyBiosource |
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 , partial) Recombinant Human Myosin-11 (MYH11) , partial |
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MBS718804-005mgMammalianCell |
MyBiosource |
0.05mg(Mammalian-Cell) |
EUR 1240 |
Recombinant Human Myosin-11 (MYH11) , partial |
|
MBS718804-05mgEColi |
MyBiosource |
0.5mg(E-Coli) |
EUR 1020 |
Recombinant Human Myosin-11 (MYH11) , partial |
|
MBS718804-05mgYeast |
MyBiosource |
0.5mg(Yeast) |
EUR 1240 |
Recombinant Human Myosin-11 (MYH11) , partial |
|
MBS718804-1mgEColi |
MyBiosource |
1mg(E-Coli) |
EUR 1380 |
Recombinant Human Myosin-11 (MYH11) , partial |
|
MBS718804-1mgYeast |
MyBiosource |
1mg(Yeast) |
EUR 1890 |
, partial) Recombinant Human Myosin-10 (MYH10), partial |
|
MBS955851-005mgBaculovirus |
MyBiosource |
0.05mg(Baculovirus) |
EUR 1920 |
Recombinant Human Myosin-10 (MYH10), partial |
|
MBS955851-01mgBaculovirus |
MyBiosource |
0.1mg(Baculovirus) |
EUR 2330 |
Recombinant Human Myosin-10 (MYH10), partial |
|
MBS955851-05mgEColi |
MyBiosource |
0.5mg(E-Coli) |
EUR 2095 |
Recombinant Human Myosin-10 (MYH10), partial |
|
MBS955851-05mgYeast |
MyBiosource |
0.5mg(Yeast) |
EUR 2210 |
Recombinant Human Myosin-10 (MYH10), partial |
|
MBS955851-1mgEColi |
MyBiosource |
1mg(E-Coli) |
EUR 2545 |
, partial) Recombinant Human Myosin-6 (MYH6), partial |
|
CSB-EP015299HU |
Cusabio |
6810 mg |
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Recombinant Human Myosin-6 (MYH6), partial |
|
CSB-EP015299HU-100ug |
Cusabio |
100ug |
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|
|
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Description: 160-816aa |
Recombinant Human Myosin-6 (MYH6), partial |
|
CSB-EP015299HU-1mg |
Cusabio |
1mg |
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|
|
Description: 160-816aa |
Recombinant Human Myosin-6 (MYH6), partial |
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CSB-EP015299HU-20ug |
Cusabio |
20ug |
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|
|
|
Description: 160-816aa |
Recombinant Human Myosin-6 (MYH6), partial |
|
CSB-EP015299HUa0 |
Cusabio |
9932 mg |
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Recombinant Human Myosin-6 (MYH6), partial |
|
CSB-EP015299HUa0-100ug |
Cusabio |
100ug |
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|
|
|
Description: 160-816aa |
Recombinant Human Myosin-6 (MYH6), partial |
|
CSB-EP015299HUa0-1mg |
Cusabio |
1mg |
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|
|
|
Description: 160-816aa |
Recombinant Human Myosin-6 (MYH6), partial |
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CSB-EP015299HUa0-20ug |
Cusabio |
20ug |
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|
|
|
Description: 160-816aa |
, partial) Recombinant Human Myosin-7 (MYH7), partial |
|
CSB-EP015300HU |
Cusabio |
9938 mg |
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Recombinant Human Myosin-7 (MYH7), partial |
|
CSB-EP015300HU-100ug |
Cusabio |
100ug |
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|
|
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Description: 1-109aa |
Recombinant Human Myosin-7 (MYH7), partial |
|
CSB-EP015300HU-1mg |
Cusabio |
1mg |
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|
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Description: 1-109aa |
Recombinant Human Myosin-7 (MYH7), partial |
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CSB-EP015300HU-20ug |
Cusabio |
20ug |
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|
|
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Description: 1-109aa |
, partial) Recombinant Human Myosin-9 (MYH9), partial |
|
CSB-EP015303HUe0 |
Cusabio |
1758 mg |
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Recombinant Human Myosin-9 (MYH9), partial |
|
CSB-EP015303HUe0-100ug |
Cusabio |
100ug |
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|
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Description: 2-241aa |
Recombinant Human Myosin-9 (MYH9), partial |
|
CSB-EP015303HUe0-1mg |
Cusabio |
1mg |
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|
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Description: 2-241aa |
Recombinant Human Myosin-9 (MYH9), partial |
|
CSB-EP015303HUe0-20ug |
Cusabio |
20ug |
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|
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Description: 2-241aa |
 ,partial) Recombinant Human Myosin-9(MYH9) ,partial |
|
AP71716 |
SAB |
1mg |
EUR 1978 |
|
|
,partial) Recombinant Human Myosin-6(MYH6),partial |
|
AP72695 |
SAB |
each |
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|
|
Recombinant Human Myosin-6 (MYH6), partial |
|
CSB-BP015299HU |
Cusabio |
10121 mg |
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Recombinant Human Myosin-6 (MYH6), partial |
|
CSB-BP015299HU-100ug |
Cusabio |
100ug |
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|
|
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Description: 160-816aa |
Recombinant Human Myosin-6 (MYH6), partial |
|
CSB-BP015299HU-1mg |
Cusabio |
1mg |
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|
|
|
Description: 160-816aa |
Recombinant Human Myosin-6 (MYH6), partial |
|
CSB-BP015299HU-20ug |
Cusabio |
20ug |
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|
|
|
Description: 160-816aa |
Recombinant Human Myosin-7 (MYH7), partial |
|
CSB-BP015300HU |
Cusabio |
9781 mg |
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Recombinant Human Myosin-7 (MYH7), partial |
|
CSB-BP015300HU-100ug |
Cusabio |
100ug |
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|
|
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Description: 1-109aa |
Recombinant Human Myosin-7 (MYH7), partial |
|
CSB-BP015300HU-1mg |
Cusabio |
1mg |
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|
|
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Description: 1-109aa |
Recombinant Human Myosin-7 (MYH7), partial |
|
CSB-BP015300HU-20ug |
Cusabio |
20ug |
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|
|
|
Description: 1-109aa |
Recombinant Human Myosin-6 (MYH6), partial |
|
CSB-YP015299HU |
Cusabio |
5861 mg |
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Recombinant Human Myosin-6 (MYH6), partial |
|
CSB-YP015299HU-20ug |
Cusabio |
20ug |
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|
|
|
Description: 160-816aa |
 , partial) Recombinant Human Myosin-2 (MYH2) , partial |
|
MBS1302144-005mgBaculovirus |
MyBiosource |
0.05mg(Baculovirus) |
EUR 1070 |
Recombinant Human Myosin-2 (MYH2) , partial |
|
MBS1302144-005mgEColi |
MyBiosource |
0.05mg(E-Coli) |
EUR 745 |
Recombinant Human Myosin-2 (MYH2) , partial |
|
MBS1302144-005mgYeast |
MyBiosource |
0.05mg(Yeast) |
EUR 905 |
Recombinant Human Myosin-2 (MYH2) , partial |
|
MBS1302144-02mgEColi |
MyBiosource |
0.2mg(E-Coli) |
EUR 990 |
Recombinant Human Myosin-2 (MYH2) , partial |
|
MBS1302144-05mgEColi |
MyBiosource |
0.5mg(E-Coli) |
EUR 1090 |
 , partial) Recombinant Human Myosin-9 (MYH9) , partial |
|
MBS718871-002mgBaculovirus |
MyBiosource |
0.02mg(Baculovirus) |
EUR 1125 |
Recombinant Human Myosin-9 (MYH9) , partial |
|
MBS718871-002mgEColi |
MyBiosource |
0.02mg(E-Coli) |
EUR 750 |
Recombinant Human Myosin-9 (MYH9) , partial |
|
MBS718871-002mgYeast |
MyBiosource |
0.02mg(Yeast) |
EUR 910 |
Recombinant Human Myosin-9 (MYH9) , partial |
|
MBS718871-01mgEColi |
MyBiosource |
0.1mg(E-Coli) |
EUR 900 |
Recombinant Human Myosin-9 (MYH9) , partial |
|
MBS718871-01mgYeast |
MyBiosource |
0.1mg(Yeast) |
EUR 1065 |
 , partial) Recombinant Human Myosin-8 (MYH8) , partial |
|
MBS717528-INQUIRE |
MyBiosource |
INQUIRE |
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 , partial) Recombinant Human Myosin-3 (MYH3) , partial |
|
MBS949055-INQUIRE |
MyBiosource |
INQUIRE |
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 , partial) Recombinant Human Myosin-7 (MYH7) , partial |
|
MBS965074-002mgBaculovirus |
MyBiosource |
0.02mg(Baculovirus) |
EUR 1030 |
Recombinant Human Myosin-7 (MYH7) , partial |
|
MBS965074-002mgEColi |
MyBiosource |
0.02mg(E-Coli) |
EUR 610 |
Recombinant Human Myosin-7 (MYH7) , partial |
|
MBS965074-002mgYeast |
MyBiosource |
0.02mg(Yeast) |
EUR 785 |
Recombinant Human Myosin-7 (MYH7) , partial |
|
MBS965074-01mgEColi |
MyBiosource |
0.1mg(E-Coli) |
EUR 705 |
Recombinant Human Myosin-7 (MYH7) , partial |
|
MBS965074-01mgYeast |
MyBiosource |
0.1mg(Yeast) |
EUR 920 |
 , partial) Recombinant Human Myosin-6 (MYH6) , partial |
|
MBS962226-002mgEColi |
MyBiosource |
0.02mg(E-Coli) |
EUR 255 |
Recombinant Human Myosin-6 (MYH6) , partial |
|
MBS962226-002mgYeast |
MyBiosource |
0.02mg(Yeast) |
EUR 270 |
Recombinant Human Myosin-6 (MYH6) , partial |
|
MBS962226-01mgEColi |
MyBiosource |
0.1mg(E-Coli) |
EUR 405 |
Recombinant Human Myosin-6 (MYH6) , partial |
|
MBS962226-01mgYeast |
MyBiosource |
0.1mg(Yeast) |
EUR 450 |
Recombinant Human Myosin-6 (MYH6) , partial |
|
MBS962226-05mgYeast |
MyBiosource |
0.5mg(Yeast) |
EUR 1065 |
 , partial) Recombinant Human Myosin-7B (MYH7B) , partial |
|
MBS1149986-INQUIRE |
MyBiosource |
INQUIRE |
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 , partial) Recombinant Rat Myosin-11 (Myh11) , partial |
|
MBS1378164-INQUIRE |
MyBiosource |
INQUIRE |
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 , partial) Recombinant Rat Myosin-10 (Myh10) , partial |
|
MBS1414792-INQUIRE |
MyBiosource |
INQUIRE |
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, partial) Recombinant Rat Myosin-9 (Myh9), partial |
|
CSB-EP717285RA |
Cusabio |
6891 mg |
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Recombinant Rat Myosin-9 (Myh9), partial |
|
CSB-EP717285RA-100ug |
Cusabio |
100ug |
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|
|
|
Description: 2-241aa |
Recombinant Rat Myosin-9 (Myh9), partial |
|
CSB-EP717285RA-1mg |
Cusabio |
1mg |
Ask for price |
|
|
|
Description: 2-241aa |
Recombinant Rat Myosin-9 (Myh9), partial |
|
CSB-EP717285RA-20ug |
Cusabio |
20ug |
Ask for price |
|
|
|
Description: 2-241aa |
 , partial) Recombinant Rat Myosin-6 (Myh6) , partial |
|
MBS1126348-INQUIRE |
MyBiosource |
INQUIRE |
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, partial) Recombinant Pig Myosin-4 (MYH4), partial |
|
MBS1354999-INQUIRE |
MyBiosource |
INQUIRE |
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 , partial) Recombinant Rat Myosin-9 (Myh9) , partial |
|
MBS1288820-002mgEColi |
MyBiosource |
0.02mg(E-Coli) |
EUR 375 |
Recombinant Rat Myosin-9 (Myh9) , partial |
|
MBS1288820-01mgEColi |
MyBiosource |
0.1mg(E-Coli) |
EUR 635 |
Recombinant Rat Myosin-9 (Myh9) , partial |
|
MBS1288820-1mgEColi |
MyBiosource |
1mg(E-Coli) |
EUR 1925 |
Recombinant Rat Myosin-9 (Myh9) , partial |
|
MBS1288820-5x1mgEColi |
MyBiosource |
5x1mg(E-Coli) |
EUR 8405 |
, partial) Recombinant Dog Myosin-9 (MYH9), partial |
|
MBS1320195-002mgBaculovirus |
MyBiosource |
0.02mg(Baculovirus) |
EUR 1290 |
Recombinant Dog Myosin-9 (MYH9), partial |
|
MBS1320195-002mgEColi |
MyBiosource |
0.02mg(E-Coli) |
EUR 970 |
Recombinant Dog Myosin-9 (MYH9), partial |
|
MBS1320195-002mgYeast |
MyBiosource |
0.02mg(Yeast) |
EUR 1080 |
Recombinant Dog Myosin-9 (MYH9), partial |
|
MBS1320195-01mgEColi |
MyBiosource |
0.1mg(E-Coli) |
EUR 1130 |
Recombinant Dog Myosin-9 (MYH9), partial |
|
MBS1320195-01mgYeast |
MyBiosource |
0.1mg(Yeast) |
EUR 1230 |
 , partial) Recombinant Rat Myosin-7 (Myh7) , partial |
|
MBS1092149-INQUIRE |
MyBiosource |
INQUIRE |
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, partial) Recombinant Pig Myosin-2 (MYH2), partial |
|
MBS1383161-INQUIRE |
MyBiosource |
INQUIRE |
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 , partial) Recombinant Rat Myosin-4 (Myh4) , partial |
|
MBS1421500-INQUIRE |
MyBiosource |
INQUIRE |
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, partial) Recombinant Pig Myosin-7 (MYH7), partial |
|
MBS966458-INQUIRE |
MyBiosource |
INQUIRE |
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, partial) Recombinant Dog Myosin-7 (MYH7), partial |
|
MBS953119-INQUIRE |
MyBiosource |
INQUIRE |
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 , partial) Recombinant Rat Myosin-3 (Myh3) , partial |
|
MBS956257-INQUIRE |
MyBiosource |
INQUIRE |
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 , partial) Recombinant Mouse Myosin-14 (Myh14) , partial |
|
MBS1284681-002mgBaculovirus |
MyBiosource |
0.02mg(Baculovirus) |
EUR 1645 |
Recombinant Mouse Myosin-14 (Myh14) , partial |
|
MBS1284681-002mgEColi |
MyBiosource |
0.02mg(E-Coli) |
EUR 1395 |
Recombinant Mouse Myosin-14 (Myh14) , partial |
|
MBS1284681-002mgYeast |
MyBiosource |
0.02mg(Yeast) |
EUR 1405 |
Recombinant Mouse Myosin-14 (Myh14) , partial |
|
MBS1284681-01mgEColi |
MyBiosource |
0.1mg(E-Coli) |
EUR 1685 |
Recombinant Mouse Myosin-14 (Myh14) , partial |
|
MBS1284681-01mgYeast |
MyBiosource |
0.1mg(Yeast) |
EUR 1655 |
 , partial) Recombinant Mouse Myosin-11 (Myh11) , partial |
|
MBS1006374-002mgBaculovirus |
MyBiosource |
0.02mg(Baculovirus) |
EUR 1075 |
Recombinant Mouse Myosin-11 (Myh11) , partial |
|
MBS1006374-002mgEColi |
MyBiosource |
0.02mg(E-Coli) |
EUR 665 |
Recombinant Mouse Myosin-11 (Myh11) , partial |
|
MBS1006374-002mgYeast |
MyBiosource |
0.02mg(Yeast) |
EUR 830 |
Recombinant Mouse Myosin-11 (Myh11) , partial |
|
MBS1006374-01mgEColi |
MyBiosource |
0.1mg(E-Coli) |
EUR 775 |
Recombinant Mouse Myosin-11 (Myh11) , partial |
|
MBS1006374-01mgYeast |
MyBiosource |
0.1mg(Yeast) |
EUR 970 |
 , partial) Recombinant Mouse Myosin-10 (Myh10) , partial |
|
MBS1413163-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
 , partial) Recombinant Bovine Myosin-10 (MYH10) , partial |
|
MBS1393245-INQUIRE |
MyBiosource |
INQUIRE |
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, partial) Recombinant Rabbit Myosin-11 (MYH11), partial |
|
MBS948611-INQUIRE |
MyBiosource |
INQUIRE |
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 , partial) Recombinant Mouse Myosin-9 (Myh9) , partial |
|
MBS1281774-005mgBaculovirus |
MyBiosource |
0.05mg(Baculovirus) |
EUR 1125 |
Recombinant Mouse Myosin-9 (Myh9) , partial |
|
MBS1281774-005mgEColi |
MyBiosource |
0.05mg(E-Coli) |
EUR 750 |
Recombinant Mouse Myosin-9 (Myh9) , partial |
|
MBS1281774-005mgYeast |
MyBiosource |
0.05mg(Yeast) |
EUR 910 |
Recombinant Mouse Myosin-9 (Myh9) , partial |
|
MBS1281774-02mgEColi |
MyBiosource |
0.2mg(E-Coli) |
EUR 1000 |
Recombinant Mouse Myosin-9 (Myh9) , partial |
|
MBS1281774-05mgEColi |
MyBiosource |
0.5mg(E-Coli) |
EUR 1100 |
, partial) Recombinant Horse Myosin-7 (MYH7), partial |
|
MBS1284539-INQUIRE |
MyBiosource |
INQUIRE |
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 , partial) Recombinant Mouse Myosin-7 (Myh7) , partial |
|
MBS1336124-INQUIRE |
MyBiosource |
INQUIRE |
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 , partial) Recombinant Mouse Myosin-4 (Myh4) , partial |
|
MBS1473570-INQUIRE |
MyBiosource |
INQUIRE |
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, partial) Recombinant Horse Myosin-2 (MYH2), partial |
|
MBS1430254-INQUIRE |
MyBiosource |
INQUIRE |
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 , partial) Recombinant Mouse Myosin-8 (Myh8) , partial |
|
MBS965620-INQUIRE |
MyBiosource |
INQUIRE |
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 , partial) Recombinant Mouse Myosin-6 (Myh6) , partial |
|
MBS952668-005mgBaculovirus |
MyBiosource |
0.05mg(Baculovirus) |
EUR 1100 |
Recombinant Mouse Myosin-6 (Myh6) , partial |
|
MBS952668-005mgEColi |
MyBiosource |
0.05mg(E-Coli) |
EUR 695 |
Recombinant Mouse Myosin-6 (Myh6) , partial |
|
MBS952668-005mgYeast |
MyBiosource |
0.05mg(Yeast) |
EUR 855 |
Recombinant Mouse Myosin-6 (Myh6) , partial |
|
MBS952668-02mgEColi |
MyBiosource |
0.2mg(E-Coli) |
EUR 905 |
Recombinant Mouse Myosin-6 (Myh6) , partial |
|
MBS952668-05mgEColi |
MyBiosource |
0.5mg(E-Coli) |
EUR 995 |
 , partial) Recombinant Mouse Myosin-3 (Myh3) , partial |
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MBS955980-INQUIRE |
MyBiosource |
INQUIRE |
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, partial) Recombinant Chicken Myosin-11 (MYH11), partial |
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MBS961625-INQUIRE |
MyBiosource |
INQUIRE |
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, partial) Recombinant Rabbit Myosin-4 (MYH4), partial |
|
MBS1295147-INQUIRE |
MyBiosource |
INQUIRE |
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 , partial) Recombinant Bovine Myosin-2 (MYH2) , partial |
|
MBS1342661-INQUIRE |
MyBiosource |
INQUIRE |
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 , partial) Recombinant Bovine Myosin-7 (MYH7) , partial |
|
MBS1398011-INQUIRE |
MyBiosource |
INQUIRE |
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, partial) Recombinant Rabbit Myosin-7 (MYH7), partial |
|
MBS965416-INQUIRE |
MyBiosource |
INQUIRE |
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, partial) Recombinant Chicken Myosin-9 (MYH9), partial |
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MBS966607-INQUIRE |
MyBiosource |
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, partial) Recombinant Chicken Myosin-3 (MYH3), partial |
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MBS950700-INQUIRE |
MyBiosource |
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 , partial) Recombinant Mouse Myosin-7B (Myh7b) , partial |
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MBS1130490-002mgBaculovirus |
MyBiosource |
0.02mg(Baculovirus) |
EUR 1555 |
Recombinant Mouse Myosin-7B (Myh7b) , partial |
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MBS1130490-002mgEColi |
MyBiosource |
0.02mg(E-Coli) |
EUR 1275 |
Recombinant Mouse Myosin-7B (Myh7b) , partial |
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MBS1130490-002mgYeast |
MyBiosource |
0.02mg(Yeast) |
EUR 1315 |
Recombinant Mouse Myosin-7B (Myh7b) , partial |
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MBS1130490-01mgEColi |
MyBiosource |
0.1mg(E-Coli) |
EUR 1535 |
Recombinant Mouse Myosin-7B (Myh7b) , partial |
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MBS1130490-01mgYeast |
MyBiosource |
0.1mg(Yeast) |
EUR 1545 |
, partial) Recombinant Mesocricetus auratus Myosin-7 (MYH7), partial |
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MBS1124826-INQUIRE |
MyBiosource |
INQUIRE |
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, partial) Recombinant Mesocricetus auratus Myosin-6 (MYH6), partial |
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MBS1166544-INQUIRE |
MyBiosource |
INQUIRE |
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) Recombinant Myosin Heavy Chain 1 (MYH1) |
|
RPA754Hu01 |
Cloud-Clone |
10ug |
EUR 170 |
Recombinant Myosin Heavy Chain 1 (MYH1) |
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RPA754Mu01 |
Cloud-Clone |
10ug |
EUR 180 |
 , partial) Recombinant Human Myosin-IIIa (MYO3A) , partial |
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MBS1460916-005mgBaculovirus |
MyBiosource |
0.05mg(Baculovirus) |
EUR 1150 |
Recombinant Human Myosin-IIIa (MYO3A) , partial |
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MBS1460916-005mgMammalianCell |
MyBiosource |
0.05mg(Mammalian-Cell) |
EUR 1405 |
Recombinant Human Myosin-IIIa (MYO3A) , partial |
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MBS1460916-05mgEColi |
MyBiosource |
0.5mg(E-Coli) |
EUR 1150 |
Recombinant Human Myosin-IIIa (MYO3A) , partial |
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MBS1460916-05mgYeast |
MyBiosource |
0.5mg(Yeast) |
EUR 1405 |
Recombinant Human Myosin-IIIa (MYO3A) , partial |
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MBS1460916-1mgEColi |
MyBiosource |
1mg(E-Coli) |
EUR 1745 |
 , partial) Recombinant Human Myosin-IIIb (MYO3B) , partial |
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MBS1430174-INQUIRE |
MyBiosource |
INQUIRE |
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 Protein) Human Myosin-1 (MYH1) Protein |
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abx168162-1ml |
Abbexa |
1 ml |
EUR 225 |
 ELISA kit) Human Myosin 1(MYH1) ELISA kit |
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E01A5966 |
BlueGene |
96T |
EUR 700 |
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Description: ELISA |
Human Myosin 1(MYH1) ELISA kit |
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E01M0413-192T |
BlueGene |
192 tests |
EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Myosin 1(MYH1) ELISA kit |
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E01M0413-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Myosin 1(MYH1) ELISA kit |
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E01M0413-48wellsplate |
BlueGene |
48 wells plate |
EUR 280 |
Human Myosin 1(MYH1) ELISA kit |
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E01M0413-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Myosin 1(MYH1) ELISA kit |
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E01M0413-96wellsplate |
BlueGene |
96 wells plate |
EUR 405 |
 ELISA Kit) Human Myosin 1 (MYH1) ELISA Kit |
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MBS7239025-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 5685 |
Human Myosin 1 (MYH1) ELISA Kit |
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MBS7239025-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 485 |
Human Myosin 1 (MYH1) ELISA Kit |
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MBS7239025-5x96StripWells |
MyBiosource |
5x96-Strip-Wells |
EUR 3020 |
Human Myosin 1 (MYH1) ELISA Kit |
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MBS7239025-96StripWells |
MyBiosource |
96-Strip-Wells |
EUR 690 |
, partial) Recombinant Human Unconventional myosin-X (MYO10), partial |
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CSB-EP884628HU |
Cusabio |
11647 mg |
Ask for price |
Recombinant Human Unconventional myosin-X (MYO10), partial |
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CSB-EP884628HU-100ug |
Cusabio |
100ug |
Ask for price |
|
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Description: 1669-1917aa |
Recombinant Human Unconventional myosin-X (MYO10), partial |
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CSB-EP884628HU-1mg |
Cusabio |
1mg |
Ask for price |
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Description: 1669-1917aa |
Recombinant Human Unconventional myosin-X (MYO10), partial |
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CSB-EP884628HU-20ug |
Cusabio |
20ug |
Ask for price |
|
|
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Description: 1669-1917aa |
 , partial) Recombinant Human Unconventional myosin-VI (MYO6) , partial |
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MBS1479859-INQUIRE |
MyBiosource |
INQUIRE |
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 CLIA Kit) Human Myosin-1 (MYH1) CLIA Kit |
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20-abx491987 |
Abbexa |
-
Ask for price
-
Ask for price
-
Ask for price
|
- 96 tests
- 5 × 96 tests
- 10 × 96 tests
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|
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 , partial) Recombinant Human Unconventionnal myosin-X (MYO10) , partial |
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MBS1376326-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
 ELISA Kit) Human Myosin-1 (MYH1) ELISA Kit |
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20-abx152417 |
Abbexa |
-
Ask for price
-
Ask for price
-
Ask for price
|
- 96 tests
- 5 × 96 tests
- 10 × 96 tests
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|
Identification of copy quantity variation of CAPN10 in Thais with sort 2 diabetes by multiplex PCR and denaturing excessive efficiency liquid chromatography (DHPLC).
Copy quantity variations (CNVs) have been proven to be related to a number of ailments. They’ll trigger deviation of genotypes from Hardy-Weinberg Equilibrium (HWE). Genetic case-control affiliation research in Thais revealed that genotype distribution of CAPN10 Indel19 was deviated from HWE after correction of genotyping error. Due to this fact, we goal to determine CNVs inside CAPN10 Indel19 area. The semi-quantitative denaturating excessive efficiency liquid chromatography (DHPLC) technique was used to detect CNVs within the area of CAPN10 Indel19 marker in cohort of 305 sufferers with sort 2 diabetes and 250 management topics with out diabetes. CNVs within the area of CAPN10 Indel19 was efficiently detected by DHPLC.
After correction of genotype calling based mostly on the standing of recognized CNVs, CAPN10 Indel19 genotypes had been well-fitted for HWE (p>0.05). Nevertheless, we didn’t discover affiliation between CNV genotypes and danger of sort 2 diabetes in our inhabitants. CNVs in CAPN10 have been recognized in Thais. These CNVs result in deviation from HWE of CAPN10 Indel19 genotypes. After excluding recognized CNVs from the evaluation, CAPN10 Indel19 was related to sort 2 diabetes. The knowledge obtained from our examine could be useful for genotyping accuracies of SNPs residing within the CNVs area.
Excessive-performance liquid chromatography underneath partially denaturing situations (dHPLC) is a quick and cost-effective technique for screening molecular defects: 4 novel mutations present in X-linked persistent granulomatous illness.
Implementing exact methods in routine analysis of persistent granulomatous illness (CGD), which expedite the screening of molecular defects, could also be essential for a fast assumption of affected person prognosis. This examine in contrast the efficacy of single-strand conformation polymorphism evaluation (SSCP) and high-performance liquid chromatography underneath partially denaturing situations (dHPLC) for screening mutations in CGD sufferers. We chosen 10 male CGD sufferers with a medical historical past of extreme recurrent infections and irregular respiratory burst operate. gDNA, mRNA and cDNA samples had been ready by customary strategies. CYBB exons had been amplified by PCR and screened by SSCP or dHPLC. Irregular DNA fragments had been sequenced to disclose the character of the mutations.
The SSCP and dHPLC strategies confirmed DNA abnormalities, respectively, in 55% and 100% of the instances. Sequencing of the irregular DNA samples confirmed mutations in all instances. 4 novel mutations in CYBB had been recognized which had been picked up solely by the dHPLC screening (c.904 insC, c.141+5 g>t, c.553 T>C, and c.665 A>T). This work highlights the relevance of dHPLC, a delicate, quick, dependable and cost-effective technique for screening mutations in CGD, which together with practical assays assessing the phagocyte respiratory burst will contribute to expedite the definitive analysis of X-linked CGD, direct therapy, genetic counselling and to have a transparent assumption of the prognosis. This technique is very appropriate for growing nations.
dHPLC effectivity for semi-automated cDNA-AFLP analyses and fragment assortment within the apple scab-resistance gene mannequin.
cDNA-AFLP evaluation for transcript profiling has been efficiently utilized to check many plant organic techniques, notably plant-microbe interactions. Nevertheless, the separation of cDNA-AFLP fragments by gel electrophoresis is reported to be labor-intensive with solely restricted potential for automation, and the gathering of differential bands for gene identification is much more cumbersome.
On this work, we current using dHPLC (denaturing excessive efficiency liquid chromatography) and automatic DNA fragment assortment utilizing the WAVE(®) System to research and recuperate cDNA-AFLP fragments.
The tactic is efficiently utilized to the Malus-Venturia inaequalis interplay, making it potential to gather 66 totally different transcript-derived fragments for apple genes putatively concerned within the protection response activated by the HcrVf2 resistance gene.
The outcomes, validated by actual time quantitative RT-PCR, had been in keeping with the plant-pathogen interplay underneath investigation and this additional helps the suitability of dHPLC for cDNA-AFLP transcript profiling. Options and benefits of this new strategy are mentioned, evincing that it’s an nearly absolutely automatable and cost-effective resolution for processing massive numbers of samples and gathering differential genes concerned in different organic processes and non-model vegetation.
The usage of DHPLC (Denaturing Excessive Efficiency Liquid Chromatography) in II degree screening of the CFTR gene in Prenatal Analysis.
OBJECTIVE
The goal of the examine is to judge the function of Denaturing Excessive Efficiency Liquid Chromatography (DHPLC) within the second degree screening of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene.
METHODS
A 9-month potential examine, between June 2008 and March 2009 at Artemisia Fetal Medical Centre, included 3829 samples of amniotic fluid collected from ladies present process mid-trimester amniocentesis.The genetic analysis of CF was based mostly on analysis of the primary mutations of the CFTR gene on fetal DNA extracted from the amniocytes, (first degree screening) utilizing totally different business diagnostic techniques. A second degree screening utilizing DHPLC, on the amniotic fluid and on a blood pattern from the couple, was supplied in case of fetuses heterozygous at first degree screening.
RESULTS
Of 3829 fetuses, 134 had been discovered to be constructive, 129 heterozygous and 5 affected. Of the 129 {couples}, following acceptable genetic counselling, 53 requested a second degree screening. By way of using DHPLC, 44 {couples} had been discovered to be adverse, and in 9 {couples}, 9 uncommon mutations had been recognized.
CONCLUSIONS
The primary degree screening could be helpful to proof as much as 75% of the CF mutations. The second degree screening can determine an additional 10% of mutant alleles. DHPLC was discovered to be a dependable and particular technique for the speedy identification of the uncommon CFTR mutations which weren’t revealed in preliminary first degree screening.
