DES-Testbed, Freie Universität Research Pigeon,Plant,plasmid,Real-time High-performance liquid chromatography under partially denaturing conditions

High-performance liquid chromatography under partially denaturing conditions


des-testbed

DHPLC/SURVEYOR nuclease: a delicate, speedy and inexpensive technique to research BRCA1 and BRCA2 mutations in breast most cancers households.

Hereditary breast most cancers accounts for about 10% of all breast cancers and BRCA1 and BRCA2 genes have been recognized as validated susceptibility genes for this pathology. Testing for BRCA gene mutations is normally based mostly on a pre-screening strategy, such because the partial denaturation DHPLC technique, and capillary direct sequencing. Nevertheless, this strategy is time consuming as a result of massive measurement of BRCA1 and BRCA2 genes. Just lately, a brand new low price and time saving DHPLC protocol has been developed to research gene mutations through the use of SURVEYOR(®) Nuclease digestion and DHPLC evaluation. A subset of 90 sufferers, enrolled within the Genetic Counseling Program of the Nationwide Most cancers Centre of Bari (Italy), was carried out to validate this strategy.

Earlier retrospective evaluation confirmed that 9/90 sufferers (10%) had been mutated in BRCA1 and BRCA2 genes and these information had been confirmed by the current strategy. DNA samples underwent landing PCR and, subsequently, SURVEYOR(®) nuclease digestion. BRCA1 and BRCA2 amplicons had been divided into teams relying on amplicon measurement to permit multiamplicon digestion. The product of this response had been analyzed on Transgenomic WAVE Nucleic Acid Excessive Sensitivity Fragment Evaluation System. The operator who carried out the DHPLC surveyor strategy didn’t know the sequencing outcomes at the moment. The SURVEYOR(®) Nuclease DHPLC strategy was in a position to detect all alterations with a sensitivity of 95%. Moreover, with a purpose to save time and reagents, a multiamplicon setting preparation was validated.

des-testbed

des-testbed

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

DLR-MYH1-Hu-96T 96T
EUR 621
  • Should the Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

RDR-MYH1-Hu-48Tests 48 Tests
EUR 500

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

RDR-MYH1-Hu-96Tests 96 Tests
EUR 692

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

RD-MYH1-Hu-48Tests 48 Tests
EUR 478

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

RD-MYH1-Hu-96Tests 96 Tests
EUR 662

Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

DLR-MYH1-Mu-48T 48T
EUR 489
  • Should the Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

DLR-MYH1-Mu-96T 96T
EUR 635
  • Should the Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

RDR-MYH1-Mu-48Tests 48 Tests
EUR 511

Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

RDR-MYH1-Mu-96Tests 96 Tests
EUR 709

Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

RD-MYH1-Mu-48Tests 48 Tests
EUR 489

Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

RD-MYH1-Mu-96Tests 96 Tests
EUR 677

Human Myosin 1(MYH1) ELISA kit

E01M0413-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Myosin 1(MYH1) ELISA kit

E01M0413-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Myosin 1(MYH1) ELISA kit

E01M0413-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human MYH1/ Myosin-1 ELISA Kit

E1687Hu 1 Kit
EUR 571

Human MYH1(Myosin-1) ELISA Kit

EH0735 96T
EUR 524.1
  • Detection range: 0.156-10 ng/ml
  • Uniprot ID: P12882
  • Alias: MYH1(Myosin Heavy Chain 1, Skeletal Muscle, Adult)/Myosin-1/Myosin heavy chain 1/Myosin heavy chain 2x(MyHC-2x)
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Pig MYH1/ Myosin-1 ELISA Kit

E0133Pi 1 Kit
EUR 717

Bovine MYH1/ Myosin-1 ELISA Kit

E0186Bo 1 Kit
EUR 717

Rat Myosin 1(MYH1) ELISA kit

E02M0413-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Myosin 1(MYH1) ELISA kit

E02M0413-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Myosin 1(MYH1) ELISA kit

E02M0413-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Myosin 1(MYH1) ELISA kit

E04M0413-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rabbit Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Myosin 1(MYH1) ELISA kit

E04M0413-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rabbit Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Myosin 1(MYH1) ELISA kit

E04M0413-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rabbit Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Myosin 1(MYH1) ELISA kit

E03M0413-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Mouse Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Myosin 1(MYH1) ELISA kit

E03M0413-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Mouse Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Myosin 1(MYH1) ELISA kit

E03M0413-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Mouse Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Myosin 1(MYH1) ELISA kit

E06M0413-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Goat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Myosin 1(MYH1) ELISA kit

E06M0413-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Goat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Myosin 1(MYH1) ELISA kit

E06M0413-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Goat Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Myosin 1(MYH1) ELISA kit

E08M0413-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Canine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Myosin 1(MYH1) ELISA kit

E08M0413-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Canine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Myosin 1(MYH1) ELISA kit

E08M0413-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Canine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Myosin 1(MYH1) ELISA kit

E07M0413-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Porcine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Myosin 1(MYH1) ELISA kit

E07M0413-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Porcine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Myosin 1(MYH1) ELISA kit

E07M0413-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Porcine Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Myosin 1(MYH1) ELISA kit

E09M0413-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Monkey Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Myosin 1(MYH1) ELISA kit

E09M0413-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Monkey Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Myosin 1(MYH1) ELISA kit

E09M0413-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Monkey Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Recombinant Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1)

4-RPA754Hu01
  • EUR 481.70
  • EUR 232.00
  • EUR 1531.36
  • EUR 577.12
  • EUR 1054.24
  • EUR 385.00
  • EUR 3678.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P12882
  • Buffer composition: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 27.0kDa
  • Isoelectric Point: 9.6
Description: Recombinant Human Myosin Heavy Chain 1, Skeletal Muscle, Adult expressed in: E.coli

Recombinant Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1)

4-RPA754Mu01
  • EUR 503.20
  • EUR 238.00
  • EUR 1612.00
  • EUR 604.00
  • EUR 1108.00
  • EUR 400.00
  • EUR 3880.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q5SX40
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 37.5kDa
  • Isoelectric Point: 5.6
Description: Recombinant Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult expressed in: E.coli

Guinea pig Myosin 1(MYH1) ELISA kit

E05M0413-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Guinea pig Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Myosin 1(MYH1) ELISA kit

E05M0413-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Guinea pig Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Myosin 1(MYH1) ELISA kit

E05M0413-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Guinea pig Myosin 1(MYH1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Myosin heavy polypeptide 1 (MYH1) polyclonal antibody

ABP-PAB-01616 100 ug Ask for price
    • Product line: Miscellaneous
    • Brand:

ELISA kit for Bovine Myosin-1 (MYH1)

KTE10236-48T 48T
EUR 354
  • Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosi
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Description: Quantitative sandwich ELISA for measuring Bovine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Bovine Myosin-1 (MYH1)

KTE10236-5platesof96wells 5 plates of 96 wells
EUR 2252
  • Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosi
  • Show more
Description: Quantitative sandwich ELISA for measuring Bovine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Bovine Myosin-1 (MYH1)

KTE10236-96T 96T
EUR 572
  • Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosi
  • Show more
Description: Quantitative sandwich ELISA for measuring Bovine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Canine Myosin-1 (MYH1)

KTE20052-48T 48T
EUR 354
  • Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosi
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Description: Quantitative sandwich ELISA for measuring Canine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Canine Myosin-1 (MYH1)

KTE20052-5platesof96wells 5 plates of 96 wells
EUR 2252
  • Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosi
  • Show more
Description: Quantitative sandwich ELISA for measuring Canine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Canine Myosin-1 (MYH1)

KTE20052-96T 96T
EUR 572
  • Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosi
  • Show more
Description: Quantitative sandwich ELISA for measuring Canine Myosin-1 (MYH1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Protein

20-abx168162
  • EUR 676.00
  • EUR 272.00
  • EUR 2068.00
  • EUR 801.00
  • EUR 481.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody

20-abx005265
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody

20-abx177671
  • EUR 1135.00
  • EUR 551.00
  • 1 mg
  • 200 ug
  • Please enquire.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody

20-abx212233
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody

20-abx212641
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody

20-abx104339
  • EUR 411.00
  • EUR 133.00
  • EUR 1135.00
  • EUR 551.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody

20-abx128730
  • EUR 398.00
  • EUR 133.00
  • EUR 1107.00
  • EUR 537.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody

20-abx173673
  • EUR 787.00
  • EUR 411.00
  • 1 mg
  • 200 ug
  • Please enquire.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody

20-abx320249
  • EUR 300.00
  • EUR 244.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody

abx235469-100ug 100 ug
EUR 551
  • Shipped within 5-12 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody

abx235470-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

abx053857-96tests 96 tests
EUR 754
  • Shipped within 5-12 working days.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

20-abx152417
  • EUR 6642.00
  • EUR 3542.00
  • EUR 825.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

abx253693-96tests 96 tests
EUR 754
  • Shipped within 5-12 working days.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

abx570236-96tests 96 tests
EUR 668
  • Shipped within 5-12 working days.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) CLIA Kit

20-abx491987
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Please enquire.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult(MYH1)ELISA Kit

QY-E03032 96T
EUR 361

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

SEA754Hu-10x96wellstestplate 10x96-wells test plate
EUR 4273.35
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10
  • Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

SEA754Hu-1x48wellstestplate 1x48-wells test plate
EUR 439.57
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10
  • Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

SEA754Hu-1x96wellstestplate 1x96-wells test plate
EUR 585.1
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10
  • Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

SEA754Hu-5x96wellstestplate 5x96-wells test plate
EUR 2332.95
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10
  • Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

4-SEA754Hu
  • EUR 4324.00
  • EUR 2283.00
  • EUR 586.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Myosin Heavy Chain 1, Skeletal Muscle, Adult elisa. Alternative names of the recognized antigen: MYHSA1
  • MYHa
  • MyHC-2X/D
  • Myosin-1
  • Myosin Heavy Chain IIx/d
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Protein

20-abx068140
  • EUR 704.00
  • EUR 286.00
  • EUR 2165.00
  • EUR 829.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody (FITC)

20-abx271252
  • EUR 453.00
  • EUR 244.00
  • EUR 1316.00
  • EUR 634.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody (Biotin)

20-abx271518
  • EUR 425.00
  • EUR 230.00
  • EUR 1219.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Antibody Pair

20-abx370650
  • EUR 1622.00
  • EUR 1038.00
  • 10 × 96 tests
  • 5 × 96 tests
  • Shipped within 5-15 working days.

Recombinant Treponema pallidum p15 (partial)

7-07552 100µg Ask for price

Recombinant Treponema pallidum p15 (partial)

7-07553 500µg Ask for price

Recombinant Treponema pallidum p15 (partial)

7-07554 1000µg Ask for price

Recombinant Treponema pallidum p17 (Partial)

7-07564 100µg Ask for price

Recombinant Treponema pallidum p17 (Partial)

7-07565 500µg Ask for price

Recombinant Treponema pallidum p17 (Partial)

7-07566 1000µg Ask for price

Recombinant Treponema pallidum p47 (Partial)

7-07576 100µg Ask for price

Recombinant Treponema pallidum p47 (Partial)

7-07577 500µg Ask for price

Recombinant Treponema pallidum p47 (Partial)

7-07578 1000µg Ask for price

Recombinant Treponema pallidum TmpA (Partial)

7-07579 100µg Ask for price

Recombinant Treponema pallidum TmpA (Partial)

7-07580 500µg Ask for price

Recombinant Treponema pallidum TmpA (Partial)

7-07581 1000µg Ask for price

MYL5 Myosin Light Chain 5 (1-173 a.a.) Human Recombinant Protein

PROTQ02045-1 Regular: 10ug
EUR 317
Description: _x000D_ MYL5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 197 amino acids (1-173 a.a.) and having a molecular mass of 22.1kDa.;MYL5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

ELISA kit for Human MYH1 (Myosin Heavy Chain 1, Skeletal Muscle, Adult)

E-EL-H1430 1 plate of 96 wells
EUR 534
  • Gentaur's MYH1 ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MYH1. Standards or samples are added to the micro ELISA plate wells and combined with th
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Human MYH1 (Myosin Heavy Chain 1, Skeletal Muscle, Adult) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human MYH1 (Myosin Heavy Chain 1, Skeletal Muscle, Adult)

ELK2107 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated ant
  • Show more
Description: A sandwich ELISA kit for detection of Myosin Heavy Chain 1, Skeletal Muscle, Adult from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) Polyclonal Antibody (Human, Pig)

4-PAA754Hu01
  • EUR 232.00
  • EUR 2285.00
  • EUR 574.00
  • EUR 289.00
  • EUR 208.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: MYH1 (Tyr585~Ala794)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Pig Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1)

Mouse Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

20-abx154425
  • EUR 6642.00
  • EUR 3542.00
  • EUR 825.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Pig Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

abx360530-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Monkey Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

abx359028-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Chicken Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

abx355635-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Rabbit Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

abx363586-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Sheep Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit

abx364394-96tests 96 tests
EUR 926
  • Shipped within 5-12 working days.

Identification of copy quantity variation of CAPN10 in Thais with sort 2 diabetes by multiplex PCR and denaturing excessive efficiency liquid chromatography (DHPLC).

 

Copy quantity variations (CNVs) have been proven to be related to a number of ailments. They’ll trigger deviation of genotypes from Hardy-Weinberg Equilibrium (HWE). Genetic case-control affiliation research in Thais revealed that genotype distribution of CAPN10 Indel19 was deviated from HWE after correction of genotyping error. Due to this fact, we goal to determine CNVs inside CAPN10 Indel19 area. The semi-quantitative denaturating excessive efficiency liquid chromatography (DHPLC) technique was used to detect CNVs within the area of CAPN10 Indel19 marker in cohort of 305 sufferers with sort 2 diabetes and 250 management topics with out diabetes. CNVs within the area of CAPN10 Indel19 was efficiently detected by DHPLC.

After correction of genotype calling based mostly on the standing of recognized CNVs, CAPN10 Indel19 genotypes had been well-fitted for HWE (p>0.05). Nevertheless, we didn’t discover affiliation between CNV genotypes and danger of sort 2 diabetes in our inhabitants. CNVs in CAPN10 have been recognized in Thais. These CNVs result in deviation from HWE of CAPN10 Indel19 genotypes. After excluding recognized CNVs from the evaluation, CAPN10 Indel19 was related to sort 2 diabetes. The knowledge obtained from our examine could be useful for genotyping accuracies of SNPs residing within the CNVs area.

 

Excessive-performance liquid chromatography underneath partially denaturing situations (dHPLC) is a quick and cost-effective technique for screening molecular defects: 4 novel mutations present in X-linked persistent granulomatous illness.

 

Implementing exact methods in routine analysis of persistent granulomatous illness (CGD), which expedite the screening of molecular defects, could also be essential for a fast assumption of affected person prognosis. This examine in contrast the efficacy of single-strand conformation polymorphism evaluation (SSCP) and high-performance liquid chromatography underneath partially denaturing situations (dHPLC) for screening mutations in CGD sufferers. We chosen 10 male CGD sufferers with a medical historical past of extreme recurrent infections and irregular respiratory burst operate. gDNA, mRNA and cDNA samples had been ready by customary strategies. CYBB exons had been amplified by PCR and screened by SSCP or dHPLC. Irregular DNA fragments had been sequenced to disclose the character of the mutations.

The SSCP and dHPLC strategies confirmed DNA abnormalities, respectively, in 55% and 100% of the instances. Sequencing of the irregular DNA samples confirmed mutations in all instances. 4 novel mutations in CYBB had been recognized which had been picked up solely by the dHPLC screening (c.904 insC, c.141+5 g>t, c.553 T>C, and c.665 A>T). This work highlights the relevance of dHPLC, a delicate, quick, dependable and cost-effective technique for screening mutations in CGD, which together with practical assays assessing the phagocyte respiratory burst will contribute to expedite the definitive analysis of X-linked CGD, direct therapy, genetic counselling and to have a transparent assumption of the prognosis. This technique is very appropriate for growing nations.

 

dHPLC effectivity for semi-automated cDNA-AFLP analyses and fragment assortment within the apple scab-resistance gene mannequin.

 

cDNA-AFLP evaluation for transcript profiling has been efficiently utilized to check many plant organic techniques, notably plant-microbe interactions. Nevertheless, the separation of cDNA-AFLP fragments by gel electrophoresis is reported to be labor-intensive with solely restricted potential for automation, and the gathering of differential bands for gene identification is much more cumbersome.

On this work, we current using dHPLC (denaturing excessive efficiency liquid chromatography) and automatic DNA fragment assortment utilizing the WAVE(®) System to research and recuperate cDNA-AFLP fragments.

The tactic is efficiently utilized to the Malus-Venturia inaequalis interplay, making it potential to gather 66 totally different transcript-derived fragments for apple genes putatively concerned within the protection response activated by the HcrVf2 resistance gene.

The outcomes, validated by actual time quantitative RT-PCR, had been in keeping with the plant-pathogen interplay underneath investigation and this additional helps the suitability of dHPLC for cDNA-AFLP transcript profiling. Options and benefits of this new strategy are mentioned, evincing that it’s an nearly absolutely automatable and cost-effective resolution for processing massive numbers of samples and gathering differential genes concerned in different organic processes and non-model vegetation.

The usage of DHPLC (Denaturing Excessive Efficiency Liquid Chromatography) in II degree screening of the CFTR gene in Prenatal Analysis.

 

OBJECTIVE

The goal of the examine is to judge the function of Denaturing Excessive Efficiency Liquid Chromatography (DHPLC) within the second degree screening of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene.

 

METHODS

A 9-month potential examine, between June 2008 and March 2009 at Artemisia Fetal Medical Centre, included 3829 samples of amniotic fluid collected from ladies present process mid-trimester amniocentesis.The genetic analysis of CF was based mostly on analysis of the primary mutations of the CFTR gene on fetal DNA extracted from the amniocytes, (first degree screening) utilizing totally different business diagnostic techniques. A second degree screening utilizing DHPLC, on the amniotic fluid and on a blood pattern from the couple, was supplied in case of fetuses heterozygous at first degree screening.

 

RESULTS

Of 3829 fetuses, 134 had been discovered to be constructive, 129 heterozygous and 5 affected. Of the 129 {couples}, following acceptable genetic counselling, 53 requested a second degree screening. By way of using DHPLC, 44 {couples} had been discovered to be adverse, and in 9 {couples}, 9 uncommon mutations had been recognized.

 

CONCLUSIONS

The primary degree screening could be helpful to proof as much as 75% of the CF mutations. The second degree screening can determine an additional 10% of mutant alleles. DHPLC was discovered to be a dependable and particular technique for the speedy identification of the uncommon CFTR mutations which weren’t revealed in preliminary first degree screening.

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